FINAL LAB EXAM Flashcards

1
Q

where does cell division occur in plants?

A

in the apical meristem and in the cambrium

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2
Q

what is the significance of mitosis?

A

mitosis is responsible for growth and repair of multicellular organisms, and reproduction in single celled organisms

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3
Q

what is mitosis?

A

is the process by which a nucleus decides to produce 2 daughter nuclei identical in genetic material

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4
Q

Diffusion

A

tendency of small particles or molecules to spread from an area of higher concentration to lower concentration

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5
Q

plasma membrane

A

surrounds the cell, controls the diffusion of all molecules in and out of the cell. it is selectively permeable

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6
Q

osmosis

A

is a special case of diffusion. it is the diffusion of water across a selectively permeable membrane from an area of low solute to high solute concentration (water moves from an area of high to low water concentration)

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7
Q

based on your test strip result, what can you conclude happened to the glucose molecules in the experiment?

A

glucose diffused out of the dialysis tube, it went from high–> low concentration

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8
Q

Observe whether or not a blue-black colour is evident and where this colour is located.

A

Blue-Black colour is present in the dialysis tube but not outside of it. the outside area remained the same

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9
Q

on the basis of the chemical test for starch, what must have happened to the iodine?

A

Iodine diffused through the dialysis tube and came in contact with starch (reason for Blue-black colour)

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10
Q

what must have happened to the starch?

A

it stayed in the same place because its too big

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11
Q

if starch is made up of glucose monomers, how would you account or the fact that the starch did not leave the bag?

A

Starch is a polymer (bigger molecule) and glucose is a monomer (much much smaller). starch is too big too pass through the plasma membrane

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12
Q

what materials diffused in opposite directions via the dialysis tubing?

A

glucose diffused out of the bag and iodine came into the dialysis tube.

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13
Q

cell membrane

A

To protect the cell from its surroundings.It also recognizes certain chemicals and molecules that can or can’t go into the cell.

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14
Q

nucleus

A

it stores the cell’s DNA, and it coordinates the cell’s activities, which include growth, intermediary metabolism, protein synthesis, and reproduction (cell division).

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15
Q

nuclear membrane

A

A nuclear membrane or envelope surrounds every nucleus. It’s composed of an inner membrane and an outer membrane separated by the perinuclear space. The nuclear membrane keeps DNA inside the nucleus and protects it from materials in the cytoplasm

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16
Q

nucleolus

A

Function: produces ribosomes then they move out and to position in on the rough endoplasmic reticulum where they are critical in protein synthesis
It is found in both animal and plant cells

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17
Q

mitochondria

A

shaped like a tub with rounded ends, it’s where sugar is converted into energy for the cell to use

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18
Q

rough endoplasmic reticulum

A

Function: produces and modifies proteins
Located next to the nucleus
Called the rough endoplasmic reticulum because it has ribosomes on the surface.
Both plant and animal cells

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19
Q

smooth endoplasmic reticulum

A

Function: lipid and carbohydrate synthesis.
Structure: has no ribosomes therefore it is smooth.
In both animal and plant cells

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20
Q

ribosomes

A

very small organelles that carry out protein production. Free floating = make proteins & attached = carry proteins out of the cell

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21
Q

Golgi apparatus

A

stack of “pancakes” membrane disks that sort and package proteins for transport within or out of cell

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22
Q

lysosome

A

One of the key organelles involved in digestion and waste removal is the lysosome. Lysosomes are organelles that contain digestive enzymes. They digest excess or worn out organelles, food particles, and engulfed viruses or bacteria. Lysosomes are like the stomach of the cell.

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23
Q

centrioles

A

found in animal cells only. may help organize microtubules assembly; a pair is found within the centrosome in animal cells (the plant cell centrosome lack centrioles)

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24
Q

Chloroplast

A

found only in plant cells. Chloroplasts are organelles found in plant cells and eukaryotic algae that conduct photosynthesis. Chloroplasts absorb sunlight and use it in conjunction with water and carbon dioxide gas to produce food for the plant

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25
Q

cell wall

A

found only in plant cells.The cell wall is the protective, semi-permeable outer layer of a plant cell. A major function of the cell wall is to give the cell strength and structure, and to filter molecules that pass in and out of the cell.

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26
Q

central vacuole

A

found only in plant cells.is a cellular organelle found in plant cells. It is often the largest organelle in the cell. It is surrounded by a membrane and functions to hold materials and wastes. It also functions to maintain the proper pressure within the plant cells to provide structure and support for the growing plant.

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27
Q

what is an ideal organism to study?

A

organisms should be easy to grow or rear, have a rapid generation time, produce many offsprings and take up little space

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28
Q

what did Mendel use for his genetic experiments?

A

Garden sweet peas because they are easy to grow, had many distinct observable characteristics + he could control mating by transferring pollen between specific flowers

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29
Q

what is the large chromosome called in a fruit fly?

A

Polytene. which can be easily seen and studied under a compound microscope.

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30
Q

what is the shape of the onion cells?

A

square or rectangular

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31
Q

how can you tell they are plant cells?

A

cell walls, large vacuoles and chloroplast id observed

32
Q

describe the shape of the nucleus and its size relative to that of the entire cell

A

circular shape, about 1/10th of a cell (10%)

33
Q

where is the nucleus actually located?

A

its always between the plasma membrane and vacuole membrane

34
Q

what microscope is necessary to see membranes?

A

electron microscope

35
Q

why are the leave of Elodea Leaf easy to study?

A

the leaves of this waterweed are only 2 cells thick, they allow light to pass through and we can study them with the compound microscope

36
Q

what is the general shape of the chloroplasts?

A

round, oval shaped

37
Q

what is it called when organelles move along the cytoskeleton inside the cells?

A

Cytoplasmic streaming or cyclosis. (cyclosis of chloroplasts is rare in plants but we can usually see it in the Elodea cells)

38
Q

what is the difference in function between the Elodea cells and the onion epidermal cells?

A

Elodea leaves have green chloroplasts spots which it needs to survive. the onion cells do not have chloroplasts because they grow underground so they don’t need to capture sunlight.

39
Q

explain how the presence or absence of certain organelles in the Elodea cells and onion cells relates to the function of each cell.

A

Since plants are stationary and lack a skeletal system, it makes sense that their cells should have rigid cell walls for support. The Elodea plant is an ideal specimen for observing the cell wall in plants and the chloroplasts which contain chlorophyll, the pigment that makes photosynthesis possible.

40
Q

what are Amyloplasts?

A

starch is stored in amyloplasts of potatoes. they are easy to see in some organs that store starch.

41
Q

what plant cell structure is seen as these lines/boxes around the amyloplasts in potatoes?

A

cell wall

42
Q

what is the term used to describe the dialysis membrane?

A

dialysis tubing or Visking tubing

43
Q

what happens to the amyloplasts when thy come in contact with iodine? based on the colour reaction that you see, what conclusion can you make about the contents of amyloplasts?

A

the starch in amyloplasts meet iodine and turn blue/purple/black. this shows that amyloplasts contain starch

44
Q

hypertonic solution

A

solution that has a greater concentration of solutes on the outside of a cell when compared with the inside of a cell.

45
Q

hypotonic solution

A

solution that has a lower osmotic pressure than another solution. this generally refers to a solution that has less solute and more water than another solution

46
Q

isotonic solution

A

refers to two solutions having the same osmotic pressure across a semipermeable membrane. This state allows for the free movement of water across the membrane without changing the concentration of solutes on either side

47
Q

plasmolysis

A

is the process in which cells lose water in a hypertonic solution.

48
Q

osmotic pressure

A

is the minimum pressure which needs to be applied to a solution to prevent the inward flow of its pure solvent across a semipermeable membrane.

49
Q

turgor pressure

A

is the force within the cell that pushes the plasma membrane against the cell wall.

50
Q

what are the 3 outstanding microscopic structural differences between the plant cells you have observed your cheek cells?

A

Onion cells are brick-like in shape while human cheek cells are rounded. Human cheek cells do not have a cell wall or a large vacuole. Both onion and human cheek cells are epithelial cells. The main difference between onion cell and human cheek cell is the shape and the structure of each type of cells.

51
Q

what are the 4 main stages of cellular respiration?

A

glycolysis, Acetyl CoA synthesis, Citric acid cycle and oxidative phosphorylation

52
Q

what is the waste product of cellular respiration?

A

water and carbon dioxide (the more CO2 generated the more energy is released)

53
Q

do all the stages of cellular respiration happen in the mitochondrion?

A

no, glycolysis occurs in the cytosol, the last 3 stages ( Acetyl CoA synthesis, citric acid cycle and electron transport chain) occur in the mitochondrion.

54
Q

how does CO2 exit the body?

A

carbon is transferred by diffusion into the blood of the animal and it is transported to the lungs where it again transfers into the lungs by diffusion and is then exhaled

55
Q

what happens when you combine CO2 from cellular respiration with water?

A
Carbonic acid (H2CO3) this Ould decrease blood pH in the following manner: 
CO2+H2O--->H2O--> H+ +HCO3-
56
Q

how can a protein denature?

A

when subjected to a change in pH will denature. which is the loss of the secondary and tertiary structure of protein. when all of these things happen, it no longer functions.

57
Q

how many layers does the onion epidermis have?

A

2 layers

58
Q

define cellular respiration

A

a set of metabolic reactions and processes that tae place in the cell on an organism to convert biochemical energy from nutrients into ATP ( adenosine triphosphate)

59
Q

what an indirect indicator that cellular respiration is happening in animals?

A

air intake or breathing

60
Q

what does the pulse oximeter do?

A

detects a person’s oxygen saturation level, which is the % of hemoglobin in arteries red blood cells that carry oxygen. it send 2 wavelengths of light through your finger. 1 wavelength detects bed blood cells with oxygen bound to hemoglobin; the other wavelength detects red blood cells with unbound hemoglobin

61
Q

pulse rate

A

the # of heart beats per minute

62
Q

ventilation rate

A

the # of inhale-exhale cycles per minute

63
Q

which measurements showed an increase in the “exercise” column?

A

ventilation rate and pulse rate

64
Q

explain why any measurements takes after exercise are higher than the readings taken at rest

A

Red blood cells are working really hard so they can pulse and ventilation needs to increase

65
Q

which measurements stayed the same in the Exercise column?

A

O2 saturation

66
Q

why did O2 saturation say the same?

A

Oxygen enters cells through simple diffusion

67
Q

define fermentation

A

a metabolic process in the absence of oxygen can transform some of the energy in organic compounds to produce ATP

68
Q

give an example of fermentation in humans

A

Muscle cells when exercising don’t get enough oxygen

69
Q

alcoholic fermentation

A

yeasts produce ethanol and are used in wine and beer making , other waste products are NADH and CO2

70
Q

what is the classic test for CO2?

A

limewater

71
Q

what happens when you combine limewater and CO2?

A

calcium carbonate (CaCO3)

72
Q

what colour does yeast turn when it produces CO2

A

white

73
Q

is CO2 present in the gas coming from the yeast? what do you think it was produced by?

A

yes, it was produced by yeast cells

74
Q

anaerobic

A

no oxygen

75
Q

any changes to colour in the test tubes labelled (C+ positive control)? (exp for ethanol)

A

yes the colour changes to a green blue. positive for ethanol

76
Q

any changes in the test tube labelled “C- for negative control? what information can you obtain from this negative control? (ethanol exp)

A

yes the colour turned yellow. negative for ethanol

77
Q

observe any changes in the experimental tubes. compare to your controls.

A

the colour is blue. which means its positive for ethanol.