Final (ch 18) Flashcards
This is the only nitrogen that is available to living systems
NH3
Nitrogen fixation converts N2 to this, with this enzyme
NH4+, nitrogenase
This is an example of an inducible enzyme that is only made when needed
nitrate reductase
NH4+ to NO2- is this process using this enzyme
nitrification
nitrate reductase
NO2- to NO3- is this process using this enzyme
nitrification
nitrite reductase
This is a metalloprotein containing Fe-S clusters and Fe-Mo cofactor
nitrogenase
this many electrons are required for nitrogen fixation
8
bacteria produce nitrate via
nitrification
some organisms convert nitrate back to N2 via
denitrification
produces glutamine from glutamate
glutamine synthetase (basic)
produces glutamate from glutamine
glutamate synthase (acidic)
N2 to NH4+
nitrogen fixation
Synthetase uses this, synthase does not
ATP
During glutamine synthetase reaction this happens
Glu is phosphorylated
ammonium ion displaces inorganic phosphate
During glutamate synthase reaction this happens
nirtogen is assimilated into citric acid cycle intermediate to produce Glu
Gln is deaminated to produce a second Glu
The conversion of alpha kg to glutamate is this type of reaction
transamination
Transaminases use this as a cofactor
pyridoxal-5’-phosphate
PLP is derived from this essential nutrient
pyridoxine (B6)
What ammino acid is attached to the PLP in a typical transaminase
lysine
This is the key to the mechansim behind transaminases
schiff base formation
what coenzyme is required for the conversion of serine to glycine
tetrahydrofolate
phenylketonuria results from a deficiency of this enzyme
phenylalanine hydroxylase
what is the amino acid that is a precursor for the neurotransmitters epinephrine and norepinephrine
tyrosine
denitrification is the conversion of this to this
nitrate to nitrogen gas
AA with any of these three things are more difficult to synthesize
sulfur
branched chains
aromatic groups
in plants and pacteria synthesis of aromatic AA involves formation of this
chorismate
Tyr is produced from PhE via this
hydroxylation
a compound diffusing directly from one active site to the other without entering the surrounding solven is called
channeling
this process increases the rate of metabolic processes by preventing the lose of intermediate
channeling
This is used in Trp synth
channeling
This is not formed from carbohydrate metabolites
histidine
Amino acids are the precursors of some of these, including
signaling molecules
GABA
Catecholamines are derived from
amino acids
this is the precursor of melatonin
serotonin
giving rise to gluconeogenic precursors, citric acid cycle intermediates are an example
glucogenic
giving rise to acetyl-CoA, used for ketogenesis or fatty acid synthesis, not used for gluconeogenesis
ketogenic
These aa have ketogenic fates
leucine
lysine
these aa have glucogenic and ketogenic fates
isoleucine phenylalanine threonine pryptophan tyrosine
Some AA are converted to this via transamination
gluconeogenic substrates
This is convered to pyruvate by a process that releases ammonia and sulfur
cysteine
Enzyme that is lacking in people with phenylketonuria
phynylalanine hydroxylase
phenylketonuria is the result of the buildup of these to substances
Phe
phenylpyruvate
alkaptonuria
black urine disease
approximately 80% of excess nitrogen is excretes as this
urea
deposits of uric acid in the joints and extremities cause this
gout
The urea cycle is linked to TCA by
fumarate
review the urea cycle on pg 13
okay
bacteria, fungi and some other organisms use this to breakdown urea
urease
a coenzyme frequently encounted in transamination reactions is
pyridoxal phosphate
This is not a glucogenic aa
leucine
a molecule cynthesized in the mitochondrial matrix and transported to the cytosol for subsequent reaction is
citrulline
how many atp equivalents are required to produce one molecule of urea from ammonia and aspartate
3
These are three excreted forms of nitrogen
ammonia
urea
uric acid