Final

Question 0

Restriction endonucleases

Answer 0

Enzymes that cut DNA at a specific sequence

Question 1

Reverse transcriptase

Answer 1

Enzyme that catalyze a synthesis of a complementary DNA (cDNA) from an RNA TEMPLATE

Question 2

DNA ligase

Answer 2

Enzyme that makes a phosphodiester bond between nucleotides on the same DNA strand

Question 3

Plasmids

Answer 3

small chromosomal circles of DNA in bacteria and yeast

Question 4

Taq polymerase

Answer 4

Synthesizes DNA from a primed DNA template at 95 degrees

Question 5

Recombinant DNA technology

Answer 5

Take a copy of a gene from one individual and placing it I the genome of a different individual

Question 6

DNA libraries

Answer 6

Collection o all DNA sequences present I a particular source

Question 7

Probing / screening a DNA library

Answer 7

Use of a labeled, known DNA fragment to hybridized (by complementary base pairing)

Question 8

Polymerase chain reaction (PCR)

Answer 8

A DNA synthesis reaction that uses known primer sequences on ether side of a target gene. Reaction is based on many cycles of DNA denaturation, primer annealing, and primer extension

Question 9

Dideoxy sequencing

Answer 9

In vitro DNA synthesis reaction that includes deoxyribonucleoside triphosphates (ddNTPs) as monomers

Question 10

Genetic mapping

Answer 10

Creation of a map showing the relative positions of genes or specific DNA sequences on chromosomes

Question 11

Sun light to light reactions to

Answer 11

Calvin cycle

Question 12

Where did most of the bacteria swarm in the light spectrum?

Answer 12

Red-orange and purple -blue

Question 13

H2O gives what to the electron transport chain

Answer 13

4 electrons and it makes ATP and 2 NADPH each run

Question 14

What goes through ATP synthase to make ATP?

Answer 14

H plus

Question 15

What are the three phases of the carbon cycle?

Answer 15

1 Fixation Of carbon dioxide
2 reduction of 3PGA and G3P
3 regeneration of RuBP from G3P

Question 16

Virus capsid

Answer 16

Exterior protein coat can contain DNA

Question 17

Why is the correct form of replication

Answer 17

Semi conservative (the original strand splits in to two strands

Question 18

In which direction does DNA synthesis

Answer 18

5 prime to 3 prime

Question 19

Is DNA synthesis exergonic or endergonic

Answer 19

Exergonic

Question 20

In DNA synthesis what connects?

Answer 20

OH on the 3 prime and the tri phosphates on the 5 prime

Question 21

Helicase

Answer 21

Catalyze a the breaking of hydrogen bonds between base pairs to open the double helix

Question 22

DNA polymerase III

Answer 22

Extends the leading strand or okasaki fragment in the lagging strand

Question 23

DNA polymerase I

Answer 23

Removes the RNA primer and replaces it with DNA

Question 24

Okasaki fragments

Answer 24

Found on the laggin strand and are fragments of DNA that are made because the DNA only synthesizes in a 5 to 3 direction

Question 25

Leading strand

Answer 25

The continuous strand because it leads into the replication fork and is synthesized continuously

Question 26

What joins Okazaki fragments?

Answer 26

DNA ligase

Question 27

What proof reads DNA after it is synthesized

Answer 27

DNA polymerase

Question 28

Where does photosynthesis take place?

Answer 28

Chloroplasts

Question 29

What gets oxidized in light reactions

Answer 29

H2O

Question 30

Where does the extra ATP come from?

Answer 30

Cyclic electron flow and it gives 3 of the ATP to Calvin cycle

Question 31

How many non cyclic ATP go into the Calvin cycle?

Answer 31

6 ATP

Question 32

What’s the P of photo system 2? What electron acceptor?

Answer 32

P680 & pheophytin

Question 33

Photosystem 1 is what p? What electron acceptor?

Answer 33

P700 & ferredoxin

Question 34

How many ATPS are used by the clavin cycle?

Answer 34

9 in one cycle

Question 35

How many turns of the Calvin cycle does it take to make one glucose or fructose?

Answer 35

2

Question 36

How many NADPH’s are used in the Calvin cycle?

Answer 36

6 as part of reduction

Question 37

How many co2 enter the Calvin cycle? What are they combined with?

Answer 37

3 and a 5 carbon RuBP to make 6 3 carbon PGA’s