Final Flashcards

You may prefer our related Brainscape-certified flashcards:
0
Q

Restriction endonucleases

A

Enzymes that cut DNA at a specific sequence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

Reverse transcriptase

A

Enzyme that catalyze a synthesis of a complementary DNA (cDNA) from an RNA TEMPLATE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

DNA ligase

A

Enzyme that makes a phosphodiester bond between nucleotides on the same DNA strand

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Plasmids

A

small chromosomal circles of DNA in bacteria and yeast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Taq polymerase

A

Synthesizes DNA from a primed DNA template at 95 degrees

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Recombinant DNA technology

A

Take a copy of a gene from one individual and placing it I the genome of a different individual

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

DNA libraries

A

Collection o all DNA sequences present I a particular source

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Probing / screening a DNA library

A

Use of a labeled, known DNA fragment to hybridized (by complementary base pairing)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Polymerase chain reaction (PCR)

A

A DNA synthesis reaction that uses known primer sequences on ether side of a target gene. Reaction is based on many cycles of DNA denaturation, primer annealing, and primer extension

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Dideoxy sequencing

A

In vitro DNA synthesis reaction that includes deoxyribonucleoside triphosphates (ddNTPs) as monomers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Genetic mapping

A

Creation of a map showing the relative positions of genes or specific DNA sequences on chromosomes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Sun light to light reactions to

A

Calvin cycle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Where did most of the bacteria swarm in the light spectrum?

A

Red-orange and purple -blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

H2O gives what to the electron transport chain

A

4 electrons and it makes ATP and 2 NADPH each run

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What goes through ATP synthase to make ATP?

A

H plus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are the three phases of the carbon cycle?

A

1 Fixation Of carbon dioxide
2 reduction of 3PGA and G3P
3 regeneration of RuBP from G3P

16
Q

Virus capsid

A

Exterior protein coat can contain DNA

17
Q

Why is the correct form of replication

A

Semi conservative (the original strand splits in to two strands

18
Q

In which direction does DNA synthesis

A

5 prime to 3 prime

19
Q

Is DNA synthesis exergonic or endergonic

A

Exergonic

20
Q

In DNA synthesis what connects?

A

OH on the 3 prime and the tri phosphates on the 5 prime

21
Q

Helicase

A

Catalyze a the breaking of hydrogen bonds between base pairs to open the double helix

22
Q

DNA polymerase III

A

Extends the leading strand or okasaki fragment in the lagging strand

23
Q

DNA polymerase I

A

Removes the RNA primer and replaces it with DNA

24
Q

Okasaki fragments

A

Found on the laggin strand and are fragments of DNA that are made because the DNA only synthesizes in a 5 to 3 direction

25
Q

Leading strand

A

The continuous strand because it leads into the replication fork and is synthesized continuously

26
Q

What joins Okazaki fragments?

A

DNA ligase

27
Q

What proof reads DNA after it is synthesized

A

DNA polymerase

28
Q

Where does photosynthesis take place?

A

Chloroplasts

29
Q

What gets oxidized in light reactions

A

H2O

30
Q

Where does the extra ATP come from?

A

Cyclic electron flow and it gives 3 of the ATP to Calvin cycle

31
Q

How many non cyclic ATP go into the Calvin cycle?

A

6 ATP

32
Q

What’s the P of photo system 2? What electron acceptor?

A

P680 & pheophytin

33
Q

Photosystem 1 is what p? What electron acceptor?

A

P700 & ferredoxin

34
Q

How many ATPS are used by the clavin cycle?

A

9 in one cycle

35
Q

How many turns of the Calvin cycle does it take to make one glucose or fructose?

A

2

36
Q

How many NADPH’s are used in the Calvin cycle?

A

6 as part of reduction

37
Q

How many co2 enter the Calvin cycle? What are they combined with?

A

3 and a 5 carbon RuBP to make 6 3 carbon PGA’s