Final Flashcards
Restriction endonucleases
Enzymes that cut DNA at a specific sequence
Reverse transcriptase
Enzyme that catalyze a synthesis of a complementary DNA (cDNA) from an RNA TEMPLATE
DNA ligase
Enzyme that makes a phosphodiester bond between nucleotides on the same DNA strand
Plasmids
small chromosomal circles of DNA in bacteria and yeast
Taq polymerase
Synthesizes DNA from a primed DNA template at 95 degrees
Recombinant DNA technology
Take a copy of a gene from one individual and placing it I the genome of a different individual
DNA libraries
Collection o all DNA sequences present I a particular source
Probing / screening a DNA library
Use of a labeled, known DNA fragment to hybridized (by complementary base pairing)
Polymerase chain reaction (PCR)
A DNA synthesis reaction that uses known primer sequences on ether side of a target gene. Reaction is based on many cycles of DNA denaturation, primer annealing, and primer extension
Dideoxy sequencing
In vitro DNA synthesis reaction that includes deoxyribonucleoside triphosphates (ddNTPs) as monomers
Genetic mapping
Creation of a map showing the relative positions of genes or specific DNA sequences on chromosomes
Sun light to light reactions to
Calvin cycle
Where did most of the bacteria swarm in the light spectrum?
Red-orange and purple -blue
H2O gives what to the electron transport chain
4 electrons and it makes ATP and 2 NADPH each run
What goes through ATP synthase to make ATP?
H plus