Fermentation

Question 1

when will bacteria ferment (2)

Answer 1

• bacteria that do respiration & fermentation will ferment if do not have a TEA for ETC
• those with no fnal ETC will always ferment

Question 2

how is ATP synthesized by fermentation

Answer 2

SL(P) eg glycolysis

Question 3

purpose of ADP

Answer 3

keep glycolysis operating. ATP used as source of nrg producing ADP

Question 4

purpose of NAD+

Answer 4

keep glycolysis operating. NADH will not be oxidized by an ETC. NADH is oxidized by pyruvate or derivative of pyruvate providing the necessary NAD+

Question 5

why do the products of fermentation vary such as H2, CO2, acids and alcohols

Answer 5

species have diff enzymes for fermentation, therefore diff products.

Question 6

what happens to products of fermentation

Answer 6

transported out/diffuse out of cell

Question 7

mixed acid fermentation produce

Answer 7

acids and gases

Question 8

homofermentative lactate fermentation produce

Answer 8

lactate

Question 9

heterofermentative lactate fermentation produce

Answer 9

lactate and other products

Question 10

purpose of phenol red broth

Answer 10

determine if species can ferment a particular carbohydrate and produce acid waste +/- gas

Question 11

whats in phenol red broth

Answer 11

single fermentable carbohydrate, pH indicator changes from red to yellow in acidic pH, Durham tube to catch any gas

Question 12

results of phenol red

Answer 12

positive is yellow +/- gas