Bacterial ETC Flashcards
most dehydrogenase and quinone complexes located where
plasma membrane
how are bacterial ETC similar to mitochondrial ETC
have at least one dehydrogenase complex linked to an oxidase complex by a quinone
to use e- donors other than NADH and succinate what must bacteria do
synthesize different dehydrogenases eg H2 dehydrogenase
for aerobic respiration what is the TEA
oxygen
for aerobic respiration what is the terminal complex
oxidase complex
fn of oxidase complex
some oxidize quinones, some cyt c. all reduce oxygen. not all translocate protons
how do the diff oxidase complex differ
differ in their affinity for oxygen
for anaerobic respiration TEA is
something other than 02
for anaerobic respiration what is the terminal complex
reducatase complexes
an eg of a reductase complex
nitrate reductase uses nitrate (NO3-) as the TEA, producing nitrite (NO2-)
what are the consequences of using diff donors and TEA
affects the reduction potential and therefore amount of free nrg. can affect number of protons translocated
diff btwn mitchrondrial and bacterial ETC
bacteria have branched ETC, synthesize more than one terminal complex. bacteria can adapt their ETC to diff growth conditions. can synthesise diff complex allowing to use diff e- donors and/or TEA
what is oxidative phosphorylation
use PMF for atp synthesis
the PMF can also be used by
MFS, TonB and MotAB
oxidase test
determine if species has cytochrome c as a component of their ETC. if reagent is oxidized by cycc turns purple
