Fatty Acid Catabolism

Question 1

1) Fatty acid activation

Answer 1

In cytosol

FA —> FA-CoA

Question 2

2) β-oxidation cycle

Answer 2

Mitochondrial matrix

FA-CoA —> acetyl - CoA

Question 3

3)citric acid cycle

Answer 3

Acetyl-CoA —> FADH2, NADH, GTP

Question 4

4) Oxidative phosphorylation

Answer 4

FADH2, NADH —> ATP

Question 5

Activation:
Bond
Product
Intermediate
Enzyme
Reaction type

Answer 5

Thioester bond
Acyl-CoA
Acyl adenylate
Acyl-CoA synthetase (requires ATP)
Esterfication

Question 6

Acyl group carrier

Answer 6

Acyl-CoA-SH can’t cross into matrix
Acyl-Carnitine forms
Carnitine acyltransferase
Carnitine translocase

Question 7

β-oxidation cycle

Answer 7

Oxidation: fatty acyl-coA —> α, β-unsaturated acyl-coA
Hydration: α, β-unsaturated acyl-coA —> β-hyroxyacyl-coA
Oxidation: β-hyroxyacyl-coA —> β-ketoacyl-CoA
Cleavage: β-ketoacyl-CoA —> acetyl-CoA and acyl-CoA

Question 8

β-oxidation cycle enzymes

Answer 8

Oxidation: acyl-CoA dehydrogenase
Hydration: enoyl-CoA hydratase
Oxidation: β-hydroxyacyl-CoA dehydrogenase
Cleavage: thiolase