Family Enterobacteriaceae general, E. Coli, Genus Shigella Flashcards
Name the antigens which are common features of all the Enterobacteriaceae family bacteria.
O-Antigen: O Ag side chains
H-Antigen: Flagella
K-Antigen: Capsule
F-Antigen: Fimbriae
P-Antigen: Pilus
Why is the family Enterobacteriaceae named this way?
Name is after the most common site where the bacteria are found
Family Enterobacteriaceae are commonly found within the normal flora of the intestines in most mammals and birds except for a couple species and one genus of birds. Can you name them?
Not found in rabbits, guinea pigs, and psittacine birds
Name the four possible modes of transmission of family Enterobacteriaceae bacteria.
Ingestion (fecal-oral)
Respiratory route
Vectors (fleas, ticks, rats, etc.)
Fomites even
Name the general morphology of Family Enterobacteriaceae bacteria including: its gram stain, oxidase results, and shape.
Gram negative bacteria, all are oxidase (-), medium-sized rods (exception is the bubonic plague), and have peritrichous flagella In addition they may contain capsules, many have fimbriae for attachment factors
How resistant are Family Enterobacteriaceae bacteria to pasteurization, disinfectants, drying, and cold?
Pasteurization: Most are killed
Disinfectant: Most are readily killed
Drying: Not very resistant
Cold: Most survive
Treatment of a bacteria from the Family Enterobacteriaceae should include what prior to decision on antibiotic?
A sensitivity test because a given isolate can be resistant to many different antimicrobials . Extensive drug resistant (XDR); Multi-Drug Resistant (MDR), and Pandrug Resistant isolates of some have become a serious problem
In looking at a glucose tube, a lactose tube, a SIM tube and a lysine decarboxylase tube (LIA tube). What biochemically is indicative of Escherichia coli?
Acid and gas from glucose Most ferment lactose Indole positive Decarboxylates lysine
List the toxins which are produced by Escherichia coli?
Endotoxins
- Major role in systemic disease
Shiga-toxin (Vasotoxin) important in both human and animal disease
Alpha hemolysin
- Member of the RTX toxin
- Important for human extraintestinal infections Beta hemolysin similar to alpha hemolysin with cleaved C-terminal AA
What is the commonly used name describing infections of Escherichia coli?
Colibacillosis
In pigs what age group is most likely effected?
Neonates (approximately 1-4 days old)
Identify a significant factor why Escherichia coli attach more readily on neonatal intestines
No flora normally found in the small intestine at birth
Upon invasion of Escherichia coli. What does the bacteria do?
After invasion it can cause septicemia. As well as, express enterotoxins –> a locall effect ETEC (enterotoxiogenic E. Coli)
What are the major toxins involved with the disease colibacillosis?
Labile toxin (LT)
Stable toxin (ST)
What is the differences between Labile toxin (LT) and Stable toxin (ST) toxins?
Labile toxin (LT) is heat labile and Stable toxin is heat stable