F10 Bioreaktorodlingar, kvantitativ analys av cell- och metabolitkoncentrationer

Question 1

sensor types:

Answer 1

Non-invasive
- Not in direct contact with the sample, non-destructive

In situ:
- Placed inside the fermenter (pH, capacitance probe)

On-line
- Analysis performed continuously and automatically (in situ: pH; ex situ: HPLC)

Off-line/At-line
- Sensors, analytical equipment not in contact with the fermentation broth, manual (HPLC)

Real time signal
- negligible delay between the measurement and the result.

Question 2

Dynamiska materialbalanser

Answer 2

• En teoretisk beskrivning av en bioreaktion
• Beskriver teoretiskt hur koncentrationen av ämnen ändrar i en process
• Beroende på mätdata och robusthet i processen kan verkligen se annorlunda ut !

Question 3

Sampling demands

Answer 3

• Representative for the content of the bioreactor
• Sterile barrier
• No change in composition from sampling to analysis
• Fast response time
• No disturbance on the fermentation process
• No change during the fermentation process
• Operational stability
• Selectivity may be introduced