Experiment 8 Flashcards

1
Q

Polymers of nucleotides

A

Nucleic Acid

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2
Q

Nucleotide comprises a ____ , a ____ , and a ____ group

A

nitrogenous base ; pentose ; phosphate

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3
Q

The ___ is bonded to the pentose via ____ at carbon 1

A

Nitrogenouse base ; N-glycosidic bond

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4
Q

The ___ is bonded to the pentose via ____ through the ____ at carbon 3 and 5

A

Phosphate ; ester bond ; -OH group

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5
Q

Individual nucleotides are bound with ___ between carbons ___ and ___

A

phosphodiester bonds ; 3 ; 5

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6
Q

Nucleic Acids can be ___ and ___

A

Deoxyribonucleic acids (DNA) ; Ribonucleic acid (RNA)

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7
Q

Structurally, in nucleic acid, DNA differs from RNA because DNA is ____ , which RNA is ____

A

double-stranded ; single stranded

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8
Q

The nitrogenous bases can be bicyclic ____ , specifically ___ and ___ ;

or monocyclic ____, like ____ (For DNA only), ____ and ___ (For RNA only)

A

purines ; adenine ; guanine ;

pyrimidines ; thymine ; cytosine ; uracil

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9
Q

The pentose can be ___ and ___

A

ribose ; deoxyribose

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10
Q

DNA contains ____

A

deoxyribose

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11
Q

RNA contains ____

A

ribose

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12
Q

From its name, nucleic acids have ____

A

acidic character

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13
Q

The acidity of nucleic acids is caused by the ____ , each contains ____ capable of ___

A

phosphate residues ; H+ ; dissociation

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14
Q

Nucleic acids are ___ - carrier of many negative charges, and makes them capable of interacting with ___ , particularly with _____ (histones) - which are carrier of positive charges

A

polyAnIons ‘ polyCatIons ; alkaline proteins

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15
Q

RNA, like all nucleic acids, is a ____

A

polyanion

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16
Q

RNA in alkaline environment, form ____ called ____ , which are ____ in water. They can be precipitated from the solution with _____

A

salts ; nucleates ; soluble ; ethanol

17
Q

What are the solvents used for the Solubility of YEAST RNA

A

Water, 95% Ethanol, diluted HCI solution, diluted NaOH solution

18
Q

Like any other macromolecules, nucleic acids can also undergo ____

A

Hydrolysis

19
Q

Hydrolysis is the breakdown of large molecules into smaller fragments when reacted to water in the presence of ____ , such as ____ or ____

A

catalysts ; enzymes ; acids

20
Q

The first stage of nucleic acid hydrolysis is its breakdown into ____

A

nucleotides

21
Q

The nucleotides are further broken down into its corresponding components, namely the ____ , ____ , and ____ residues

A

nitrogenous bases ; pentose ; phosphate

22
Q

The mixture obtained after hydrolysis is called _____

A

hydrolysate

23
Q

What are the solvents used for HYDROLYSIS OF DNA and RNA

A

RNA: yeast RNA, 10% H2SO4
DNA: yeast DNA, 10% H2SO4

24
Q

If acid hydrolysis was successfully done, it is safe to assume that the hydrolysate contains free form of ____

A

nucleotide’s components

25
Q

What are the CHEMICAL TESTS FOR THE COMPONENTS OF NUCLEOTIDES

A

> Test for Purine (Nitrogenous Base)
Test for Pentose (Molisch Test, Bial’s Test)
Test for Phosphate

26
Q

> What are the reagents used for Test for Purine (Nitrogenous Base)

> What is its positive formation ___ , which is an insoluble complex of ___ and ___

A

> Hydrolysates from DNA and RNA
Ammoniacal Silver Nitrate solution
NH4OH

> white precipitate ; silver ion ; purines

27
Q

> What are the reagents used for Molisch Test

> What is its positive formation ___ , in the junction between _____

A

> Hydrolysates from DNA and RNA
Molisch Reagent
Concentrated H2SO4

> Appearance of Purple Ring ; 2 liquids

28
Q

> What are the reagents used for Bial’s Test

> What is its positive formation

A

> Hydrolysates from DNA and RNA
Bial’s Reagent

> Bluish Green color

29
Q

> What are the reagents used for Test for Phosphate

> What is its positive formation

A

> Hydrolysates from DNA and RNA
NH4OH solution
10% HNO3
0.2 ammonium molybdate solution

> Yellow precipitate

30
Q

PERFECT THE EXAM!!

A

Noted.