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Biochem Lab > Experiment 7 and 8 > Flashcards

Experiment 7 and 8 Flashcards

1
Q

Esters of long chain fatty acids and alcohols

A

Lipids

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2
Q

Building block of lipids

A

Fatty acids

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3
Q

Lipids are defined as ____ organic compound insoluble to ____ solvents, but soluble in ____ solvents

(Eg: Ether, Chloroform, Benzene, Acetone)

A

non-polar ; polar ; organic

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4
Q

It represents in cell structure and has a structural function in the cell (presents in all cell membranes)

A

Biological Role of Lipids

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5
Q

An essential source of energy, reservoir of energy

A

Biological Role of Lipids

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6
Q

esters of fatty acids with glycerols (eg: fats and waxes)

A

Simple Lipids

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7
Q

____ the simplest and most common fat which is a form in which lipids are stored in the cell.

Also known as ____

A

Triacylglycerol (TAG) ; neutral fat

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8
Q

Lipids that are linking with the other compounds such as proteolipids, phospholipids, and glycolipids.

A

Compound Lipids

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9
Q

_______ It contains glycerol, fatty acid, Nitrogenous base, and phosphoric acid.

Also known as ____

A

Compound Lipids ; conjugated lipids

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10
Q

They are substances that are soluble in lipid or derived from above groups of lipids by hydrolysis.

(Eg: Cholesterol, Sphingosine, Glycerophosphatides)

A

Derived Lipids

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11
Q

___ - Structurally defines as triester of glycerol and 3 fatty acids

____ and ____ - What are the mixture of this

A

Triacylglycerol ; fats ; oils

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12
Q

Tell me if miscible or immiscible
Water + Coconut Oil = ____
Chloroform + Coconut Oil = ____

Tell me if soluble or insoluble
Water + Cholesterol = _________
Chloroform + Cholesterol = ________

A

immiscible ; miscible

insoluble ; soluble

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13
Q

Tell me if translucent or not

Coconut Oil = ____
Glycerol = ____
Ethanol = ____
Ether = _____

(NOTE: Translucent = non-volatile)

A

translucent (non-volatile)
translucent (non-volatile)
not (volatile)
not (volatile)

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14
Q

Used to detect the presence of glycerol or fat

A

Acrolein Test

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15
Q

Tell me the color of PPT and its odor
Coconut Oil = ______
Glycerol = ______

A

black ppt (burnt fat)
no ppt (burnt fat)

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16
Q

When fat is treated STRONGLY in the presence of dehydrating agent like _____ , the glycerol portion of the molecule is dehydrated to form an _____ aldehyde, acrolein that has a pungent ____ oder

A

Potassium Bisulfate (KHSO4) ; unsaturated ; irritating

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17
Q

_________ to determine the iodine value of fats and oils and thus estimate the unsaturation of fats and oils

A

Test for Unsaturation (Iodine number)

18
Q

The higher the iodine number, the ____ unsaturated fatty acids bonds are present in a FAT

A

more

19
Q

The unsaturation is in a form of ______ which react with iodine components

A

double bonds

20
Q

Tell me its positive color for Iodine Number
Coconut Oil = ________
Oilive Oil = ________
CottonSeed Oil = ________

A
  • Dark Orange (less unsaturated)
  • Orange to brown
  • Light Orange to colorless (highly unsaturated)
21
Q

Test for rancidity causes ____ oil to be acidic compared to fresh oils

A

rancid

22
Q

Tell me the color of litmus paper and its inference
Fresh coconut Oil =
Rancid Coconut Oil =

A

Red-Red ; Blue-blue (neutral)

Blue-Red ; Red-Red (acidic)

23
Q

For Salkowski’s Test, its color is from ___ to ___ indicating the presence of ____

A

yellow ; brick red color ; cholesterol

24
Q

For Salkowski’s Test, there may be red color in the upper layer (_____) ; and green fluorescence in the lower layer due to the HEAVIER _____ setting

A

Chloroform ; sulfuric acid

25
Q

Liebermann Burchard Test gives ___ color in the presence of ____. The color is due to the ___ group of cholesterol and the ____ found in the ADJACENT FUSED ____

A

green ; cholesterol ; OH ; unsaturation ; RING

26
Q

In Liebermann Burchard Test, the color change is ____. First, it appears as a ____ coloration, changing later to ___ , and finally to deep green

A

gradual ; pink ; lilac

27
Q

ETHER dissolves ____ from the food and can be isolated from the sample.
ETHER is an ideal solvent as it is easy to remove via _____

A

fat ; evaporation

28
Q

The MORE unsaturated a fat or oil is, the ____ viscous it is due to LESSER INTERMOLECULAR ATTRACTIONS between molecules

A

less

29
Q

Tell me if its viscousity and color
Potato Chips = _________
Peanut Oil = _________

A

VERY VISCOUS (brown-dark/brown)
LESS VISCOUS (light brown-colorless)

30
Q
  • Yeast RNA is ____ in dil. HCl because of its ____ resistant
    Yeast RNA is ____ in ethanol because of its ____ dielectric
    Yeast RNA is ____ in dil. NAOH because of its ____ base. This leads to formation of ____ charged species which interacts with water molecules through ______ interaction
A

insoluble; highly
insoluble ; low
soluble ; strong ; negatively ; ion-dipole

31
Q

Basic Steps in DNA Extraction (IN ORDER)
1. Mashing or Grinding
the LARGER the surface area, the _____ the extraction procedure

A

M- L - AS - AC - I
FASTER

32
Q

Basic Steps in DNA Extraction (IN ORDER)
2. Lysis of Cells
membrane lipids are removed in the sample by adding a _____

A

M- L - AS - AC - I
detergent

33
Q

Basic Steps in DNA Extraction (IN ORDER)
3. Addition of saline solution
saline solution helps the DNA strands to ___ to each other and also causes proteins and carbohydrates to _________

A

M- L - AS - AC - I
stick ; precipitate

34
Q

Basic Steps in DNA Extraction (IN ORDER)
4. Addition of cold alcohol
DNA is ____ to alcohols, usually with ethanol or isopropanol. Draws water molecule ____ from DNA, causing it to collapse and precipitate

A

M- L - AS - AC - I
insoluble ; away

35
Q

Basic Steps in DNA Extraction (IN ORDER)
5. Isolate the DNA from the solution
the isolated DNA appears like a ________

A

M- L - AS - AC - I
white muccos

36
Q

PURINES
with Ammoniacal Silver Nitrate Solution + NH4OH

A

White PPT

37
Q

PENTOSES
with a-naphthol in ethanol + conc. Sulfuric Acid

A

Violet Ring

38
Q

PENTOSES
with Orcinol + conc. HCl + 10% FeCl3

A

Bluish-green solution

39
Q

PHOSPHATES
with 10% HNO3 + ammonium molybdate

A

Yellow PPT

40
Q

PERFECT THE EXAM!!

A

Noted.

Biochem Lab (5 decks)

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