EXPERIMENT 6 DEMO OF COMMON SERO REACTIONS Flashcards

1
Q

INSOLUBLE ANTIGEN

A

agglutinogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

antibody

A

agglutinin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

observable Ag ab reax

A

lysis, agglutination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

centrifugation rule for agglutination reaction tube method, and hemolysis

A

15 seconds 3,400 rpm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

mnemoninc for macroscopic evaluation of agglutination

A

OSMSTN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

One solid aggregate, clear background

A

4+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Several large aggregates, clear background

A

3+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Medium – sized agglutinates, clear background

A

2+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Small agglutinates, turbid background

A

1+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Tiny agglutinates, turbid background

A

W+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

No agglutination or hemolysis

A

0

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

No agglutination or hemolysis

A

0

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

intact cell button with clear supernatant

A

No hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

presence of cell button with pink supernatant

A

Partial hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

absence of cell button with red supernatant

A

Complete hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

single antigenic deteminant on cell surface

A

sensitization

17
Q

sum of interactions, multiple antigenic determinants

A

lattice formation

18
Q

AB increase

A

prozone

19
Q

AG increase

A

postzone

20
Q

electrostatic interaction

A

non covalent interaction

21
Q

best agglutinin

A

IgM

22
Q

cold reacting 4-22C

A

IgM

23
Q

warm reacting 37C

A

IgG

24
Q

time of incubation

A

15-60mins

25
Q

most sensitive technique of serologic reax, directly measure binding of Ag ab

A

primary serologic reax

26
Q

less sensitive but easier to perform, detect consquence of Ag Ab reax

A

secondary serologic reax

27
Q

examples of primary sero reax

A

ELISA (enzyme), RIA (radiosiotope), western blot (fluorescent dyes)

28
Q

examples of sec reax

A

precipitaion, agglutination, neutralization, complement activation

29
Q

initial force of attraction bet single fab site and single epitope

A

affinity