EXPERIMENT 6 DEMO OF COMMON SERO REACTIONS Flashcards
INSOLUBLE ANTIGEN
agglutinogen
antibody
agglutinin
observable Ag ab reax
lysis, agglutination
centrifugation rule for agglutination reaction tube method, and hemolysis
15 seconds 3,400 rpm
mnemoninc for macroscopic evaluation of agglutination
OSMSTN
One solid aggregate, clear background
4+
Several large aggregates, clear background
3+
Medium – sized agglutinates, clear background
2+
Small agglutinates, turbid background
1+
Tiny agglutinates, turbid background
W+
No agglutination or hemolysis
0
No agglutination or hemolysis
0
intact cell button with clear supernatant
No hemolysis
presence of cell button with pink supernatant
Partial hemolysis
absence of cell button with red supernatant
Complete hemolysis
single antigenic deteminant on cell surface
sensitization
sum of interactions, multiple antigenic determinants
lattice formation
AB increase
prozone
AG increase
postzone
electrostatic interaction
non covalent interaction
best agglutinin
IgM
cold reacting 4-22C
IgM
warm reacting 37C
IgG
time of incubation
15-60mins
most sensitive technique of serologic reax, directly measure binding of Ag ab
primary serologic reax
less sensitive but easier to perform, detect consquence of Ag Ab reax
secondary serologic reax
examples of primary sero reax
ELISA (enzyme), RIA (radiosiotope), western blot (fluorescent dyes)
examples of sec reax
precipitaion, agglutination, neutralization, complement activation
initial force of attraction bet single fab site and single epitope
affinity
