EXPERIMENT 4 SCREENING TEST FOR PHAGOCYTIC ENGULFMENT

Question 1

CULTURE SHOULD NOT BE MORE THAN __ MCFARLAND STANDARD IN CNCENTRATION

Answer 1

0.5

Question 2

angle for blood smear prep

Answer 2

25 degf

Question 3

if lymphocytes are the only blood cell seen, bacterial suspension is too___

Answer 3

heavy

Question 4

phagocytic process

Answer 4

physical contact, phagosome, phagolysosome, digestion, release

Question 5

indirect interaction

Answer 5

opsonization

Question 6

examples of opsonins

Answer 6

C3b, IgG1, IgG3

Question 7

direct interaction

Answer 7

PRRs / pattern recognition receptors to PAMPs pathogen associated molecular patterns

Question 8

superoxides

Answer 8

nitric oxide, oxygen independent mechanism, oxygen dependent mechanism

Question 9

secondary inflammation

Answer 9

when bacteria isnt phagocytized

Question 10

happens when pathogen escapes 2nd site

Answer 10

bacteremia

Question 11

autoimmune inappropriate activation of phagocytic process

Answer 11

rheumatoid arthritis, miltiple sclerosis, graves disease

Question 12

this disease has decreased synthesis of the PPF or phagocytosis promoting factor

Answer 12

chronic granulomatous disease or CGD

Question 13

what are the different PPF

Answer 13

fibronectin, complement C3, complement factor B

Question 14

test for CGD

Answer 14

nitroblue tetrazolium test

Question 15

negative result for nitroblue tetrazolium test

Answer 15

colorless turns deep blue

Question 16

indication of no color change