EXPERIMENT 3 SAMPLE PREP FOR SERO

Question 1

what is the concentration employed for anticoagulated RCS

Answer 1

2-5%

Question 2

why do we inactivate serum?

Answer 2

to inactivate the complement

Question 3

RCS is PRBC washed with saline for how many times

Answer 3

3x

Question 4

would you refrigerate the sample if the plasma is not yet separated from the cells?

Answer 4

no

Question 5

rule for centrifugation of anticoagulated blood and clotted blood

Answer 5

5-10 mins at 2500 rpm

Question 6

time for complete clotting

Answer 6

20-30mins not exceeding 60mins

Question 7

invertion of clot activator tube

Answer 7

5-6 times

Question 8

invertion of serum separator tube

Answer 8

8-10 times

Question 9

should you discard the clot?

Answer 9

no

Question 10

until when can you centrifuge samples?

Answer 10

within 2 hrs of collection

Question 11

what do you call the process when you would put the serum to water bath set at 56C for 30 mins?

Answer 11

serum inactivation

Question 12

whats the volume of the RCS?

Answer 12

less or equal to 1 mL

Question 13

until what mark will you fill tube with NSS?

Answer 13

10mL

Question 14

invertion of RCS + NSS

Answer 14

2-3 x

Question 15

how many times woul you wash the mixed solution

Answer 15

until supernatant is clear