Experiment #4: Ion-Exchange Chromatography

Question 1

Purpose of Chromatography

Answer 1

To separate, purify, quantify and identify components based on characteristics (size, shape, charge, solubility)

Question 2

Ion Chromatography

Answer 2

Interchange of ions between a stationary phase and a mobile phase (separates based on charge)

Question 3

What type of resin was used?

Answer 3

A cation exchange resin was used

Question 4

What happens to ions with a high binding affinity?

Answer 4

They bind to the resin

Question 5

What happens to ions with a weaker binding affinity?

Answer 5

The ions “release, bind, release” as they move through the resin

Question 6

How is the resin prepared?

Answer 6

It is soaked in a strong acid to ensure all binding sites are filled with H+ (HCl)

Question 7

Procedure (Ion-exchange chromatography)

Answer 7

Drain DI, test pH (make sure neutral)
Add 10mL of test solution to top of column, put flask underneath, release drops 1/s
Add DI in little bits until 50mL used

Question 8

Stationary Phase

Answer 8

A solid, gel or immobilized liquid

Question 9

Chromatographic bed

Answer 9

The column/sheet that supports the stationary phase

Question 10

Mobile phase

Answer 10

Solvent that carries samples through the stationary phase

Question 11

Delivery system

Answer 11

Force that moves mobile phase through stationary phase

Question 12

Detector

Answer 12

Way to identify/quantify substance as it elutes

Question 13

Eluate

Answer 13

The sample leaving the column

Question 14

Eluent

Answer 14

The sample loaded into the column

Question 15

Binding affinity

Answer 15

How strongly an ion pulls to a binding site