EXPERIMENT 1: EQUIPMENTS AND MARIALS IN THE HEMATOLOGY LABORATORY

Question 1

• Capillary or Dermal Puncture

Answer 1

Skin Puncture

Question 2

Skin Puncture Materials

Answer 2

1. Dry and wet cotton
2. Blood lancet
3. Capillary tube or microtube

Question 3

Syringe Method Materials

Answer 3

1. Hypodermic syringe and needle

2. Tourniquet

Question 4

Evacuated Tube System Materials

Answer 4

1. Adapter
2. Two-way needle
3. Tourniquet
4. Collection tubes

Question 5

• Feather lancet

Answer 5

Skin Puncture

Question 6

o Pointed portion is inserted

Answer 6

• Triangular blade

Question 7

• Triangular blade Standard depth

Answer 7

2mm

Question 8

Skin Puncture Tests

Answer 8

A. Microhematocrit preparation
B. Peripheral blood smear
C. Cell counts

Question 9

Skin Puncture Steps

1. Collect sample
2. Seal with [?]
3. Label
4. Centrifugation

Answer 9

sealing clay/paraffin wax

Question 10

o High-speed centrifugation

o Separation of layers

Answer 10

• Microhematocrit centrifuge/microfuge

Question 11

o Separation of layers

Answer 11

 Plasma

 Buffy coat (RBC, WBC, Platelets)

Question 12

• Microhematocrit centrifuge/microfuge

* Microhematocrit reader

Answer 12

Skin Puncture

Question 13

1. Hypodermic syringe and needle

• Standard length:

Answer 13

1 to 1.5 inch

Question 14

• Bore:
o [?] – adult
o [?] – pediatric

Answer 14

21 to 22 gauge

23 gauge

Question 15

1. Hypodermic syringe and needle parts

Answer 15

• Plunger

* Nozzle

Question 16

• Attachment

* Need to be secured/tightened

Answer 16

• Nozzle

Question 17

• [?] (max volume: 12cc)

Answer 17

10 cc

Question 18

• ?

Answer 18

5cc

Question 19

• [?], highly elastic – pediatric

Answer 19

Brown rubber torniquet

Question 20

2. Tourniquet ideal angle

Answer 20

30 to 45 degrees

Question 21

1. Collect 12 mL
2. Release tourniquet and fist
3. Apply cotton
4. Remove needle
5. Fish-out needle
6. Remove needle from the nozzle
7. Transfer blood to EDTA tube (nozzle touches the tube)
8. Invert 8 times

Answer 21

Syringe Method

Question 22

Evacuated Tube System Materials

Answer 22

1. Adapter
2. Two-way needle
3. Tourniquet
4. Collection tubes

Question 23

to avoid carry-over

Answer 23

• ORDER OF DRAW

Question 24

dispense blood in tubes before clot formation

Answer 24

• ORDER OF DISPENSING

Question 25

• ORDER OF DRAW

Question 26

• ORDER OF DISPENSING

Answer 26

``` o Blue (1st) o EDTA (2nd) o Yellow (3rd) ```

Question 27

Acid-Hematin Method

Answer 27

* Sahli pipette * Sahlisquare pipette * Comparator block

Question 28

Drabkin’s Method

Answer 28

* 13 x 100mm * Autopipette * Spectrophotometer

Question 29

Hemoglobin Determination

Answer 29

Acid-Hematin Method | Drabkin’s Method

Question 30

Cell Counts (RBC, WBC, Platelet)

Answer 30

* Thomas pipette * Sacking tube * Hemocytometer * Microscope * Tally counter

Question 31

o 1,110mm | o Improved Neubauer

Answer 31

• Hemocytometer

Question 31

o 1,110mm | o Improved Neubauer

Answer 31

• Hemocytometer

Question 32

* Glass slides * Blood sample (skin puncture; EDTA) * Dry and stain preparation * Wash, dry, observe * Differential counter (100 max) * For observing red cell morphology

Answer 32

Peripheral Blood Smear or Differential Count

Question 33

– hematology (w/ fixative)

Answer 33

o Wright’s Stain

Question 34

1. Solution A – [?] 2. Solution B – [?] 3. Solution C – [?]

Answer 34

eosin methylene blue fixative or buffer solution

Question 35

• Measures the distance travelled by red cell

Answer 35

Erythrocyte Sedimentation Rate

Question 36

* [?] – smaller | * [?] – larger

Answer 36

Winthrobe tube | Westergren tube

Question 37

• Incubated at room temperature for 4 hrs

Answer 37

Erythrocyte Sedimentation Rate

Question 38

• Rack is used to avoid disturbing of prep

Answer 38

Erythrocyte Sedimentation Rate

Question 39

• Evaluates integrity of red cell membrane

Answer 39

Osmotic Fragility Test

Question 40

* Test tube rack | * 14 x 75mm test tubes

Answer 40

Osmotic Fragility Test

Question 41

o Tube number caused hemolysis

Answer 41

o 14 (12)

Question 42

• Not routine

Answer 42

Reticulocyte count

Question 43

aim to stain cells while alive; freshly collected

Answer 43

• Supravital Stain

Question 44

• Supravital Stain

Answer 44

1. New methylene blue (NMB) | 2. Brilliant Cresyl Blue (BCB)