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Hematology 1 [LAB] > EXPERIMENT 1: EQUIPMENTS AND MARIALS IN THE HEMATOLOGY LABORATORY > Flashcards

EXPERIMENT 1: EQUIPMENTS AND MARIALS IN THE HEMATOLOGY LABORATORY Flashcards

1
Q

• Capillary or Dermal Puncture

A

Skin Puncture

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2
Q

Skin Puncture Materials

A
  1. Dry and wet cotton
  2. Blood lancet
  3. Capillary tube or microtube
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3
Q

Syringe Method Materials

A
  1. Hypodermic syringe and needle

2. Tourniquet

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4
Q

Evacuated Tube System Materials

A
  1. Adapter
  2. Two-way needle
  3. Tourniquet
  4. Collection tubes
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5
Q

• Feather lancet

A

Skin Puncture

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6
Q

o Pointed portion is inserted

A

• Triangular blade

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7
Q

• Triangular blade Standard depth

A

2mm

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8
Q

Skin Puncture Tests

A

A. Microhematocrit preparation
B. Peripheral blood smear
C. Cell counts

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9
Q

Skin Puncture Steps

  1. Collect sample
  2. Seal with [?]
  3. Label
  4. Centrifugation
A

sealing clay/paraffin wax

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10
Q

o High-speed centrifugation

o Separation of layers

A

• Microhematocrit centrifuge/microfuge

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11
Q

o Separation of layers

A

 Plasma

 Buffy coat (RBC, WBC, Platelets)

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12
Q
  • Microhematocrit centrifuge/microfuge

* Microhematocrit reader

A

Skin Puncture

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13
Q
  1. Hypodermic syringe and needle

• Standard length:

A

1 to 1.5 inch

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14
Q

• Bore:
o [?] – adult
o [?] – pediatric

A

21 to 22 gauge

23 gauge

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15
Q
  1. Hypodermic syringe and needle parts
A
  • Plunger

* Nozzle

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16
Q
  • Attachment

* Need to be secured/tightened

A

• Nozzle

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17
Q

• [?] (max volume: 12cc)

A

10 cc

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18
Q

• ?

A

5cc

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19
Q

• [?], highly elastic – pediatric

A

Brown rubber torniquet

20
Q
  1. Tourniquet ideal angle
A

30 to 45 degrees

21
Q
  1. Collect 12 mL
  2. Release tourniquet and fist
  3. Apply cotton
  4. Remove needle
  5. Fish-out needle
  6. Remove needle from the nozzle
  7. Transfer blood to EDTA tube (nozzle touches the tube)
  8. Invert 8 times
A

Syringe Method

22
Q

Evacuated Tube System Materials

A
  1. Adapter
  2. Two-way needle
  3. Tourniquet
  4. Collection tubes
23
Q

to avoid carry-over

A

• ORDER OF DRAW

24
Q

dispense blood in tubes before clot formation

A

• ORDER OF DISPENSING

25
Q

• ORDER OF DRAW

A
26
Q

• ORDER OF DISPENSING

A 
o	Blue (1st)
o	EDTA (2nd) 
o	Yellow (3rd)
27
Q

Acid-Hematin Method

A
  • Sahli pipette
  • Sahlisquare pipette
  • Comparator block
28
Q

Drabkin’s Method

A
  • 13 x 100mm
  • Autopipette
  • Spectrophotometer
29
Q

Hemoglobin Determination

A

Acid-Hematin Method

Drabkin’s Method

30
Q

Cell Counts (RBC, WBC, Platelet)

A
  • Thomas pipette
  • Sacking tube
  • Hemocytometer
  • Microscope
  • Tally counter
31
Q

o 1,110mm

o Improved Neubauer

A

• Hemocytometer

31
Q

o 1,110mm

o Improved Neubauer

A

• Hemocytometer

32
Q
  • Glass slides
  • Blood sample (skin puncture; EDTA)
  • Dry and stain preparation
  • Wash, dry, observe
  • Differential counter (100 max)
  • For observing red cell morphology
A

Peripheral Blood Smear or Differential Count

33
Q

– hematology (w/ fixative)

A

o Wright’s Stain

34
Q
  1. Solution A – [?]
  2. Solution B – [?]
  3. Solution C – [?]
A

eosin
methylene blue
fixative or buffer solution

35
Q

• Measures the distance travelled by red cell

A

Erythrocyte Sedimentation Rate

36
Q
  • [?] – smaller

* [?] – larger

A

Winthrobe tube

Westergren tube

37
Q

• Incubated at room temperature for 4 hrs

A

Erythrocyte Sedimentation Rate

38
Q

• Rack is used to avoid disturbing of prep

A

Erythrocyte Sedimentation Rate

39
Q

• Evaluates integrity of red cell membrane

A

Osmotic Fragility Test

40
Q
  • Test tube rack

* 14 x 75mm test tubes

A

Osmotic Fragility Test

41
Q

o Tube number caused hemolysis

A

o 14 (12)

42
Q

• Not routine

A

Reticulocyte count

43
Q

aim to stain cells while alive; freshly collected

A

• Supravital Stain

44
Q

• Supravital Stain

A
  1. New methylene blue (NMB)

2. Brilliant Cresyl Blue (BCB)

Hematology 1 [LAB] (8 decks)

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