Color-Coded Tubes Flashcards

1
Q

Tests: Blood culture
Additive/s: Sodium polyanethol sulfonate
Number of inversions: 8 – 10 times
Specimen: Whole blood

A

STERILE TUBE

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2
Q

Tests: Coagulation tests
Additive/s: Sodium citrate (3.2% or 3.8%) – because of the preservation of labile factors
Number of inversions: 3 - 4 times
Specimen: Whole blood

A

COAGULATION TUBES

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3
Q

Test: Specialized platelet testing
▪ Contains sodium citrate, theophylline, adenosine and dipyridamole
▪ Minimizes in vitro platelet activation and the artificial entry of platelet factors
into plasma

A

Glass CTAD tube with a light blue Hemogard closure

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4
Q
  • Thrombin and soybean trypsin inhibitor
    ▪ Providing serum for determinations of certain fibrin degradation products
A

Special blue stopper

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5
Q

Tests: Stat and routine chemistry tests, ammonia, electrolytes, arterial blood gases
Additive/s: Silica (clot activator), none in glass
Number of inversions: 5 times for plastic tubes; none for glass tubes
Specimen: Serum after centrifugation

A

SERUM TUBES (WITH OR WITHOUT CLOT ACTIVATOR/GEL)
a. Red top

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6
Q

Tests: Most chemistry tests; “stat” tests
Additive/s: Spray-coated silica (to increase platelet activation) and a polymer barrier gel
Number of inversions: 5 times, complete clotting within 30 minutes
Specimen: Serum after centrifugation

A

SERUM TUBES (WITH OR WITHOUT CLOT ACTIVATOR/GEL)
b. Serum-separator tubes: Gold BD Hemogard closure or Red-gray (Tiger-speckled)

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7
Q

Function: Discard tube – non-additive for coagulation studies or when using a winged
blood collection set
Number of inversions: None

A

SERUM TUBES (WITH OR WITHOUT CLOT ACTIVATOR/GEL)
c. Red/Light Gray and Clear top

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8
Q

Tests: Chemistry (stat tests), Serology, Blood bank
Additive/s: Heparin with sodium, lithium or ammonium ion
Number of inversions: 5 – 10 times (depending on the manufacturer)
Specimen: Whole blood

A

HEPARIN TUBES (WITH OR WITHOUT GEL PLASMA SEPARATOR)
a. Green

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9
Q

Tests: Chemistry with use of plasma (most especially potassium because heparin
prevents the release of potassium by platelets during clotting)
Additive/s: Lithium heparin and Polymer gel
Number of inversions: 8 times
Specimen: Whole blood

A

HEPARIN TUBES (WITH OR WITHOUT GEL PLASMA SEPARATOR)
b. Light Green or Green-gray: Plasma separator tubes

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10
Q

Tests: Routine hematology procedures (CBC, sedimentation rate)
Additive/s: Spray-dried K2EDTA or Liquid K3EDTA; Powdered Na2EDTA
Number of inversions: 8 times
Specimen: Whole blood

A

EDTA TUBES
a. Lavender (Purple) top

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11
Q

Tests: Blood bank (Compatibility testing)
Additive/s: K2EDTA
Number of inversions: 8 times
Specimen: Whole blood

A

EDTA TUBES
b. Pink top

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12
Q

Tests: Molecular diagnostics but can be used for MI panels and ammonia levels,
depending on the test methodology and instrumentation
Additive/s: Spray-coated K2EDTA
Number of inversions: 8 times
Specimen: Whole blood

A

PEARL/WHITE TOP: PLASMA PREPARATION TUBES

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13
Q

Tests: Lactic acid, Glucose tolerance test, Fasting blood sugar, Blood alcohol levels
Additive/s: Anti-glycolytic agent (Sodium fluoride or Iodoacetate) and Potassium oxalate
or Na2EDTA
Number of inversions: 8 times
Specimen: Whole blood

A

GLYCOLYTIC INHIBITOR TUBES

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14
Q

Tests: Stat serum chemistry determinations and samples from patients receiving
anticoagulant therapy
Additive/s: Thrombin – clots within 5 minutes
Number of inversions: 8 times
Specimen: Serum

A

THROMBIN-BASED TUBES
a. Yellow/gray and Orange top

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15
Q

Tests: Stat serum tests
Additive/s: Thrombin-based medical clotting agent – clots within 5 minutes and
separation gel
Number of inversions: 5 times
Specimen: Serum

A

THROMBIN-BASED TUBES
b. Orange top: Rapid serum Tests (RSTs)

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16
Q

Tests: Lead determination
Additive/s: K2EDTA
Number of inversions: 8 times
Specimen: Whole blood

A

SPECIAL COLLECTION TUBES
1. TAN

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17
Q

Tests: Toxicology, trace metals, nutritional analysis
Additive/s: Spray-coated silicon clot activator or; K2EDTA or Heparin
Number of inversions: 8 times
Specimen: Serum or plasma

A

ROYAL BLUE

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18
Q

Tests: Cellular studies in blood bank, human leukocyte antigen (HLA) phenotyping
and DNA and paternity testing
Additive/s: Acid Citrate Dextrose
Number of inversions: 8 times
Specimen: Whole blood

A

YELLOW

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19
Q

Tess: Westergren sedimentation rate
Additive/s: Buffered sodium citrate (4:1 ratio)
Number of inversions: 8 times
Specimen: Whole blood

A

BLACK

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20
Q

Tests: Whole blood molecular diagnostics testing;
Additive/s: Sodium citrate and Polyester gel and a Density gradient liquid
Number of inversions: 8 times
Specimen: Whole blood

A

LIGHT BLUE/BLACK: CELL PREPARATION TUBES (CPT)

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21
Q

Tests: Whole blood molecular diagnostics testing
Additive/s: Sodium heparin and a Polyester gel and Density gradient liquid
Number of inversions: 8 times
Specimen: Whole blood

A

RED/GREEN

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22
Q

Sodium citrate
Coagulation

A

Light blue

23
Q

None
Chemistry, Blood Bank, Serology/Immunology

A

Red

24
Q

Clot activator
Chemistry

A

Red

25
Q

None
NA (discard tube only)

A

Red/light gray

26
Q

no given answer in additive and department

A

Clear

27
Q

Clot activator and gel separator
Chemistry

A

Red/black (tiger)
Gold
Red/gold

28
Q

Lithium heparin and gel separator
Chemistry

A

Green/gray
Light green

29
Q

Lithium heparin, Sodium heparin, Ammonium heparin
Chemistry

A

Green

30
Q

EDTA
Hematology

A

Lavender
(purple)

31
Q

EDTA
Blood Bank

A

Pink

32
Q

Sodium fluoride and potassium, oxalate, Sodium fluoride and EDTA, Sodium fluoride

Chemistry

A

Gray

33
Q

Thrombin
Chemistry

A

Orange
Gray/yellow

34
Q

None, EDTA, Sodium heparin

Chemistry

A

Royal blue

35
Q

EDTA

no department given

A

Tan

36
Q

Sodium polyanethol sulfonate (SPS)
Microbiology

A

Yellow

37
Q

Acid citrate dextrose (ACD)

Blood Bank/Immunohematology

A

Yellow

38
Q

Yellow SPS
Sterile media bottles

A

Blood cultures (sterile
collections)

39
Q

Minimizes chance of microbial contamination

A

Blood cultures (sterile
collections)

40
Q

Light blue

A

Coagulation tubes

41
Q

The first additive tube in the order because all
other additive tubes affect coagulation tests

A

Coagulation tubes

42
Q

Red

A

Glass nonadditive
tubes

43
Q

Prevents contamination by additives in other
tubes

A

Glass nonadditive
tubes

44
Q

Red

A

Plastic clot activator
tubes

45
Q

Filled after coagulation tests because silica
particles activate clotting and affect coagulation tests (carryover of silica into
subsequent tubes can be overridden by
anticoagulant in them)

A

Plastic clot activator
tubes

46
Q

Red and gray rubber
Gold plastic

A

Serum separator tubes
(SSTs)

47
Q

Filled after coagulation tests because silica
particles activate clotting and affect
coagulation tests (carryover of silica into
subsequent tubes can be overridden by
anticoagulant in them)

A

Serum separator tubes
(SSTs)

48
Q

Green and gray rubber
Light green plastic
Green

A

Plasma separator
tubes (PSTs)
Heparin tubes

49
Q

Heparin affects coagulation tests and
interferes in collection of serum specimens; it
causes the least interference in tests other
than coagulation tests

A

Plasma separator
tubes (PSTs)
Heparin tubes

50
Q

Lavender, pink, or
purple

A

EDTA tubes

51
Q

Pearl top

A

Plasma preparation
tubes (PPTs)

52
Q

Responsible for more carryover problems
than any other additive; elevates Na and K
levels, chelates and decreases calcium and
iron levels, elevates PT and PTT results

A

EDTA tubes
Plasma preparation
tubes (PPTs)

53
Q

Gray

A

Oxalate/fluoride tubes

54
Q

Sodium fluoride and potassium oxalate affect
sodium and potassium levels, respectively.
Filled after hematology tubes because
oxalate damages cell membranes and
causes abnormal RBC morphology. Oxalate
interferes in enzyme reactions

A

Oxalate/fluoride tubes