Exp 8: Det Of Glucose

Question 0

Methods used in the past

Answer 0

Cu & ferricyanide reduction methods

Question 1

One of the first substances determined in blood

Answer 1

Glucose

Question 2

But in recent years, other methods have been widely used, including ———— ones have become popular

Answer 2

Enzymatic

Question 3

Most chemical msrmnts of glucose depend upon its —————

Answer 3

Reducing properties

Question 4

————- has been established as the specific non-enzymatic method for the determination of glucose

Answer 4

Orthotoluidine method

Question 5

Simple, rapid, and sensitive because of th intense color that develops

Answer 5

Ortho-toluidine method

Question 6

The ortho-toluidine method is based on

Answer 6

The condensation of aldosaccharides (like glucose) with an aromatic amine and glacial acetic acid.

Question 7

When heated with glucose, a solution of the aromatic amine, orthotoluidine in strongly acid solution produces a colored product whc is a ——————–

Answer 7

Stable green color

Question 8

Specimen

Answer 8

Unhemolyzed serum

Question 9

Reagents

Answer 9

Ortho-toluidine rgt

Working glucose std, 200mg%

Question 10

1. Prepare 3 test tubes to contain the ff solutions:

Answer 10

Water
Sample - 0.1cc
Glucose std - 0.1cc
OT rgt - 4.00cc

Unknown: sample 0.1cc + OTrgt 4cc
Std: Gluc std 0.1cc + OT rgt
Blank: water .1cc + OT rgt

Question 11

2. Make sure water bath is ——————- before adding rgt

Answer 11

VIGOROUSLY BOILING

Question 12

3. Mix well. Boil for ————-

Answer 12

8 minutes

Question 13

4. Cool in —— water for ——- minutes

Answer 13

Tap water, 2-3 minutes

Question 14

5. Read reabsorbance at ———- against rgt blank at —– temp

Answer 14

640nm, room temp

Question 15

Computation

Answer 15

O.D. Unknown
mg%Glucose = ———————– x 200mg%
O.D. Standard

Question 16

Conversion to S.I units

Answer 16

umol/L glucose = mg%glucose x 0.055