EXP #3 | Proteins Flashcards

1
Q

Are comprised of amino and carboxylic acids functional groups

A

Amino Acids

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2
Q

The (1) __________ and (2) __________ comprise the functional groups in a class of biologically important compounds, amino acids

A

(1) amino
(2) carboxylic acid groups

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3
Q

Are polymers composed of amino acids that are linked together by a peptide bond

A

Proteins

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4
Q

Proteins are (1) __________ composed of amino acids that are linked together by a (2) __________

A

(1) polymers
(2) peptide bond (amide bond)

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5
Q

The complete (1) __________ of proteins yields the component (2) __________

A

(1) hydrolysis
(2) amino acids

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6
Q

The (1) __________ present in amino acids and proteins react with a variety of reagents to form (2) __________

A

(1) functional groups
(2) colored products

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7
Q

The color reactions have been used as basis for the __________ of proteins and amino acids.

A

quantitative estimation

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8
Q

The structures of proteins are so much more __________ compared to those of carbohydrates and lipids

A

complex

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9
Q

The structure of proteins are described in four different ways:

(1) __________
(2) __________
(3) __________
(4) __________

A

(1) Primary
(2) Secondary
(3) Tertiary
(4) Quaternary

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10
Q

Different types of __________ between the R groups and the peptide backbone maintain protein structures

A

intermolecular forces of attraction

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11
Q

Different types of intermolecular forces of attraction between the __________ and the __________ maintain protein structures

A

R groups; peptide backbone

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12
Q

The biological activity of a protein depends on its __________ and __________

A

secondary and tertiary structures

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13
Q

A process called (1) __________ is associated with the alteration of these structures which results in changes in (2) __________ (precipitation or coagulation) and __________

A

(1) protein denaturation
(2) physical properties; loss of biological activity

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14
Q

Denaturation may be caused by the action of _______, _______, and _______

A

heat; light; chemical reagents

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15
Q

The Ninhydrin test is a test for __________

A

amino groups

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16
Q

The presence of a __________ is the positive result for the Ninhydrin test

A

blue or violet-colored solution

17
Q

The Biuret test is a test for __________

A

proteins

18
Q

The presence of a __________ is the positive result for the Biuret test

A

blue-violet solution

19
Q

The Sakaguchi test is a test for __________

A

arginine

20
Q

The presence of a __________ is the positive result for the Sakaguchi test

A

red-colored solution

21
Q

The Hopkins-Cole test is a test for __________

A

tryptophan

22
Q

The presence of a __________ is the positive result for the Hopkins-Cole test

A

Deep violet or purple ring

23
Q

The Heat Coagulation test is a test for __________

A

proteins (albumin & globulin)

24
Q

The presence of __________ is the positive result for the Heat Coagulation test

A

dense coagulum

25
Q

Waste generated from the following test should be disposed in metal-containing waste:

(1) __________
(2) __________
(3) __________

A

(1) Biuret test
(2) Hopkins-Cole test
(3) Lead Sulfide test

26
Q

Waste generated from the following test should be disposed in non-halogenated waste:

(1) __________
(2) __________

A

(1) Ninhydrin test
(2) Heat Coagulation test

27
Q

Waste generated from the following test should be disposed in halogenated organic waste:

(1) __________

A

(1) Sakaguchi test