EXP #3 | Proteins Flashcards
Are comprised of amino and carboxylic acids functional groups
Amino Acids
The (1) __________ and (2) __________ comprise the functional groups in a class of biologically important compounds, amino acids
(1) amino
(2) carboxylic acid groups
Are polymers composed of amino acids that are linked together by a peptide bond
Proteins
Proteins are (1) __________ composed of amino acids that are linked together by a (2) __________
(1) polymers
(2) peptide bond (amide bond)
The complete (1) __________ of proteins yields the component (2) __________
(1) hydrolysis
(2) amino acids
The (1) __________ present in amino acids and proteins react with a variety of reagents to form (2) __________
(1) functional groups
(2) colored products
The color reactions have been used as basis for the __________ of proteins and amino acids.
quantitative estimation
The structures of proteins are so much more __________ compared to those of carbohydrates and lipids
complex
The structure of proteins are described in four different ways:
(1) __________
(2) __________
(3) __________
(4) __________
(1) Primary
(2) Secondary
(3) Tertiary
(4) Quaternary
Different types of __________ between the R groups and the peptide backbone maintain protein structures
intermolecular forces of attraction
Different types of intermolecular forces of attraction between the __________ and the __________ maintain protein structures
R groups; peptide backbone
The biological activity of a protein depends on its __________ and __________
secondary and tertiary structures
A process called (1) __________ is associated with the alteration of these structures which results in changes in (2) __________ (precipitation or coagulation) and __________
(1) protein denaturation
(2) physical properties; loss of biological activity
Denaturation may be caused by the action of _______, _______, and _______
heat; light; chemical reagents
The Ninhydrin test is a test for __________
amino groups
The presence of a __________ is the positive result for the Ninhydrin test
blue or violet-colored solution
The Biuret test is a test for __________
proteins
The presence of a __________ is the positive result for the Biuret test
blue-violet solution
The Sakaguchi test is a test for __________
arginine
The presence of a __________ is the positive result for the Sakaguchi test
red-colored solution
The Hopkins-Cole test is a test for __________
tryptophan
The presence of a __________ is the positive result for the Hopkins-Cole test
Deep violet or purple ring
The Heat Coagulation test is a test for __________
proteins (albumin & globulin)
The presence of __________ is the positive result for the Heat Coagulation test
dense coagulum