Exercise 1 Flashcards
The amount that the image of an object is enlarged
Magnification
Extent to which object detail in an image is preserved during the magnifying process
Resolving power
The degree to which image details stand out against their background
Contrast
What do lenses do?
Focus radiation emanating from a specimen to produce the image of that specimen and on a surface sensitive to the radiation
The maximum useful magnification on a light microscope
1000x
Dyes
Usually added to sections of biological specimens to increase contrast
Eyepiece
Ocular(s)
Look like mini telescopes
Objectives
Function: magnifying
Objective
Knobs on side of scope
Focusing knobs
Bring slide into focus
Focusing knobs
Holds slide
Stage
2nd lends below the stage
Condensed
Focus light on the slide
Condenser
Circular glass on base
Light source
Provide light to see slide
Light source
Objective Magnifying Power (ObMP) for low microscope
4x
Objective Magnifying Power (ObMP) for medium microscope
10x
Objective Magnifying Power (ObMP) for high dry microscope
40x
Objective Magnifying Power (ObMP) for oil immersion microscope
100x
Total Magnifying Power for low power microscope
40x
Total Magnifying Power for medium power microscope
100x
Total Magnifying Power for high dry microscope
400x
Total Magnifying Power for oil immersion microscope
1000x
Numerical aperture (NA) for low power microscope
0.10
Numerical aperture (NA) for medium power microscope
0.25
Numerical aperture (NA) for high dry microscope
0.65
Numerical aperture (NA) for oil immersion microscope
1.25
Was the image right side up or upside down compared to the specimen?
Upside down
The image is ______ with respect to the specimen
Inverted
Shape of the renal corpuscles
Round
Shape of the nephron tubules
Oval
Increases resolving power by using electrons in a vacuum and magnetic lenses instead of light and glass lenses
Transmission electron
Preservation of greater specimen detail allows for magnifications up to 1,000,000x more
Transmission electron
