Exam revision.. Flashcards
If a tube has a cap colour of orange + vacutainer colour of green…
- What is the anticoagulant inside?
- What will this blood be used for?
- Lithium heparin
- Biochemistry
If a tube has a cap colour of pink + vacutainer colour of purple…
- What is the anticoagulant inside?
- What will this blood be used for?
- EDTA (Ethylenediamine tetra-acetic acid)
- Haematology
If a tube has a cap colour of yellow + vacutainer colour of grey…
- What is the anticoagulant inside?
- What will this blood be used for?
- Fluoride oxalate
- Glucose
If a tube has a cap colour of blue + vacutainer colour of blue…
- What is the anticoagulant inside?
- What will this blood be used for?
- Sodium/lithium citrate
- Coagulant profiles
If a tube has a cap colour of white or brown + vacutainer colour of red…
- What is the anticoagulant inside?
- What will this blood be used for?
- No anticoagulant!
- Serum collection
If a blood tube has a pink cap..
- What is the anticoagulant inside?
- What will this blood be used for?
- EDTA (Ethylenediamine tetra-acetic acid)
- Haematology
If a blood tube has a purple cap..
- What is the anticoagulant inside?
- What will this blood be used for?
- Sodium Citrate
- Coagulation/Prothombin test
If a blood tube has a white cap..
- What is the anticoagulant inside?
- What will this blood be used for?
- No anticoagulant
- All serum tests
If a blood tube has a orange cap..
- What is the anticoagulant inside?
- What will this blood be used for?
- Lithium Heparin
- Biochemsitry
If a blood tube has a yellow cap..
- What is the anticoagulant inside?
- What will this blood be used for?
- No anticoagulant
- Blood glucose
If a serum or plasma sample has turned a pink/red colour, what may this indicate?
Haemolysis
If a serum or plasma sample has turned a yellow colour, what may this indicate?
Jaundice
(Icteric)
If a serum or plasma sample has turned a milky- white colour, what may this indicate?
Lipaemia
Name the 3 Romanowsky stains used for blood smears
- Leishmann’s
- Giemsa
- Diff-Quick
Name the Supravital stain used for blood smears
Methylene blue
Name the 2 most common types of stains used for blood smears
- Romanowsky
- Supravital
What setting + how long should you spin a PCV sample in a Centrifuge for?
10,000 rmp for 5 mins
What type of tube should you use for a PCV?
Microhaematocrit tube
What is the piece of equipment you should use to measure a PCV?
Hawlsley micro-haematocritc reader
What is the normal PCV for a dog?
37 - 55%
What is the normal PCV for a cat?
24 - 45 %
What may an increased PCV indicate?
Dehydration
What may an decreased PCV indicate?
- Anaemia
- Haemorrhage
If a patient has:
* Increased PCV
* Increased TS
* Increased USG
What may be the possible cause?
Dehydration
If a patient has:
* Normal or decreased PCV
* Normal TS
* Decreased USG
What may be the possible cause?
Renal failure
If a patient has:
* Increased PCV
* Normal TS
* Normal USG
What may be the possible cause?
Polycythemia
What breed of dog is Polycythemia normal in?
Greyhounds
If a patient has:
* Normal PCV
* Increased TS
* Normal USG
What may be the possible cause?
Hyperglobulinaemia
If a patient has:
* Normal PCV
* Decreased TS
* Normal USG
What may be the possible cause?
Hypoglobulinaemia
If a patient has:
* Decreased PCV
* Normal TS
* Normal USG
What may be the possible cause?
Chronic anaemia
If a patient has:
* Decreased PCV
* Decreased TS
* Normal USG
What may be the possible cause?
Haemorrhage
What are the normal Total Solid ranges for Dogs?
52 - 82 g/L
What are the normal Total Solid ranges for Cats?
57 - 89 g/L
What are the normal ranges of blood Lactate for both Cats + Dogs?
< 2.5 mmol/L
(mmol/L)
What are the normal BUN ranges for a Dog?
2.5 - 9.6 mmol/L
What are the normal BUN ranges for a Cat?
5.7 - 12.9 mmol/L
What are the normal Creatinine ranges for a dog?
44 - 159 mol/L
What are the normal Creatinine ranges for a cat?
71 - 212 mmol/L
What year was the Ionizing Radiation Regulations created?
Updated in 2017
(orginally ‘99)
What year was the Health and Saftey at Work Act created?
1974
What did the radiation eye doses decrease to in the IRR’17?
150 > 30 % MSP
What green + white book did the BVA introduce, in response to IRR’17 for veterinary proffesionals?
Shortened form of the IRR’17, with guidance notes for the safe use of Ionizing radiation in the VP
What is the area within the x-ray tube that contains the anode + cathode?
Glass envelope
What is the area within the x-ray tube that transfers heat away from the anode?
Insulating oil
What is the area within the x-ray tube that is a flat sheet, that covers the window which absorbs any soft x-rays?
Aluminium filter
What is the area within the x-ray tube that disperses heat?
Cooling fins
What is the area within the x-ray tube that is a space where all air or gases have been extracted, allowing electrons to travel in a straight line?
Vaccum
What is the area within the x-ray tube that acts as a conductor of heat and draws the heat away from the tungesten target?
Copper stem
What is the area within the x-ray tube that is part of a low energy circuit in the cathode, that when heated, releases electrons?
Filament
What is the area within the x-ray tube that is a recessed area, where the filament lies, directing the electrons toward the anode?
Focusing cup
How are x-rays produced?
- Within the tube head houses the negatively charged Cathode + postively charged Anode
- The Cathode consists of a Filament made of Tungsten
- The filament is heated by an electric current which produces electrons
- The electrons are released by thermionic emmision
- The electrons travel at high speed across the tube from the Cathode to the Anode
- The electrons hit the Target area + 1% of x-rays are created + 99% of heat is produced
What does PACS stand for?
Picture Archiving Communication System
What is Teleradiology?
Sending an diagnostic image to refferals or specialists
What types of contrast media are avaliable?
- Positive
- Negative
- Double
Give 2 examples of positive contrast mediums
- Barium Sulphate
- Water-soluble Iodine
Give 2 examples of negative contrast mediums
- Gas
- Air
Give 2 examples of double contrast mediums
- Iodine + Air
- Barium + Air
- H20 + Air
What is a Double contrast medium?
Use of a positive + negative contrast medium together
Name an advantage + disadvantage of using the postitive contrast medium, Barium Sulphate
Advantage
1. Non-invasive
2. Laxative if constipated
3. Water-soluble doesn’t do any damage
Diadvantage
1. If leaks, will leak into any damaged tissues + spaces
Name an advantage + disadvantage of using the postitive contrast medium, Water-soluble Iodine
Advantages
1. Can be excreted by the kidneys
Disadvantages
1. Can’t be used conscious
2. Can cause fall in BP
3. More expensive
4. Difficult to administer
5. Can cause anaphylaxis
6. If a powder, can be mixed badly
Which type of contrast medium would you use if you were to perform a Double contrast Cystogram?
Water-soluble Iodine + Air (or Co2)
What precautions should be wary of when using Water-soluble Iodine + Air for a Cystogram?
- Don’t want to rupture the bladder
- Monitor for anaphylaxis
Essay Q..
A dog has presented to the VP with symptoms associated with an Intestinal perforation.
The VS has decided to perform a series of GI radiographs to confirm the dx.
You have been asked to prepare for the following surgery.
- How would you prepare the px?
- What equipment would you need?
- What contrast medium would you use?
Px preparation:
* Gain owner consent
* Fast for 12 hrs
* Take out to toilet
* Enema or bladder expression
* Pain relief given
* Check vitals
* Sedation
Equipment:
* Contrast medium
* Needle + syringe
* PPE
* Sedation/pain relief drugs
* X-ray machine
* Funnel
* Dosing syringe
* Jug/beaker
* Feeding tube to provide CM
* Positioning aids
Contrast medium choice:
* Water-soluble Iodine (+)
* As there is a GI perforation, so Barium Sulphate is contraindicated
Essay Q…
A puppy has been brought into the VP for investigation.
The main CS is anuria.
The VS admits the puppy for further investigation for a double contrast Cystogram.
- How would you prepare the px?
- What equipment is required?
- What contrast medium should be used?
The VS has asked you to assist with the taking of a urine sample.
- When should you take this in the day + why?
That should be a 4 ^ but won’t let me change it
Px preparation:
* Gain owner consent
* Fast for 12 hrs
* Take out to toilet
* Enema or bladder expression
* Pain relief given
* Check vitals
* Sedation
Equipment:
* Contrast medium
* Needle + syringe
* PPE
* Sedation/pain relief drugs
* X-ray machine
* Funnel
* Dosing syringe
* Jug/beaker
* Feeding tube to provide CM
* Positioning aids
Contrast medium choice:
* Water-soluble Iodine (+) + Gas
Urine sample:
* Take free-catch 1st thing in the morning, after the 1st couple of seconds, so no sediment is present
* Take the sample prior to the contrast procedure as the contrast medium will create a high USG
* Will also create a false-positive for the contrast medium
How does an Ultrasound work?
- Vibrating crystals in transducer
- Make soundwaves
- Ultrasonic soundwaves bounce off objects
- Changes the shape of the crystals
- Machine anaylses the image
- Creates an image (+ frozen for dx)
What equipment do you need for an Ultrasound?
- Coupling gel - takes out air
- Clippers
- Transducer
- Ultrasound computer + control panel
- Paper towels
- Trolly
- Extra pair of hands
How would you prepare a px for an Ultrasound?
- Consent - make sure dog isn’t a show dog
- Fast px for 12hr (food)
- If abdo - toilet break, if urinary - keep bladder full!
- Toilet break, manual expression, enema - if req
- Clip coat of area
- Clean site with warm Hibiscrub + Surgical spirit (to de-grease)
- Use coupling gel
5.Use machine + freeze + save image
6.Wipe off with paper towel
What are somatic affects of radiation?
Whole body changes from Ionizing radiation
What are the 2 types of Endoscopes?
- Flexible
- Rigid
Name the parts of the endoscope
- Eye piece
- Probe
- Lens
- Tip
- Handle - air, water + suction
- Blue button = air + water
- Red button = suction
- Fibre optic light source
- Instrument channel
- Guide wire
- Biopsy channel
- Umbilicus
What would you use a flexible endoscope for?
- Any internal tubular/flexible structures
- Upper + lower GI tract
- Respiratory tract
- Urinary tract
How would you clean a flexible endoscope?
- Pressure test with pump + buttons
- Wash with sterile distilled water
- Use cleaning wire throughout whole structure x3
- Soak in enzymatic cleaner
- Hang up to drip-dry
Name the extra parts of the rigid Endoscope, that differ from the flexible endoscope
- Power source
- Lens
- Camera
- Relay system
- Lens
- Fibre optic light source
- Umbilicus
- Eye piece
When is a rigid Endoscope used?
- Non-tubular channels
- Nose
- Ears
- Joints (Arthroscopy)
- For Birds, Fish + Reptiles
Identify several somatic changes that IR can cause
- Redness
- Corneal damage
- Cataracts
- Blistering
- Dehydration
- GI upset
- Skin cracking
How many years does it take for IR causing Leukaemia to show clinical signs?
20 - 30 years
Name the 3 potential hazardous sources or IR, produced from x-rays?
- Tube head
- Primary beam
- Scatter/secondary radiation
Identify 7 things that can be done to reduce scattered radiation
- PPE
- Grids
- ALARA
- Collimate
- FFD
- Stay 2m away
- Reduce KV + mAS
Who can be a RPS?
Head or Senior nurses
At what point does the controlled area cease to be the controlled area?
- If the red light is OFF
- Demarkated
- Power source is disconnected
Describe the controlled area
- 2x lights - 1x orange, 1x red
- Thick lead-lined walls
- Big enough to stay 2m away from the primary beam/source of radiation
- Demarkation - warning signs
- Where x-rays are performed!
Name 3 different devices used to asses the exposure of radiation to staff
- Dosimeters (Film-badge)
- PEDs (Personal Electronic Dosimeter)
- Thermonic dosimeter
What is the difference between a PEDs, Film-badge Dosimeter + Thermonic Dosimeters?
PEDs
* Lasts longer
* Detects real-time exposure (as electronic)
Film-badge dosimeter
* More common
* Checked Q8-12 week
* Contains small metal filters that absorbs IR
Thermonic dosimeter
* Less common
* Lithium Chloride sensitive crystals inside
* Can put in the building to give an exposure rating
Where should dosimeters be worn?
- On the trunk
- Under a lead apron
What is the normal Glucose parameters for a dog?
4.11 - 7.94 mmol/L
What is the normal Glucose parameters for a cat?
4.11 - 8.83 mmol/L
Why should you never drawback on a plunger when you are in a vein?
This will collapse the vein
Why should you always transfer venepunctured blood to a collecting tube ASAP?
To prevent contamination + clotting
Why should you always expel blood gently into a tube, after blood collection?
To prevent haemolysis/cell damage
How long does it take for blood to clot after contact with tissue, tissue fluids, glass or plastic?
10 - 20 seconds
How much blood should you collect, from a venepuncture?
Enough to fill the required level within the collection tube
What is phelbitis?
Inflammation at the vein
(/site of venepuncture)
What are the 3 layers that are present within a microhaematocrit tube, once it has been spun down in a centrifuge, for a PCV reading?
- RBCs (Erythrocytes)
- Buffy coat
- Plasma
How do you read a PCV from a Hawksley Micro-haematocrit Reader?
- Place tube into slot, sealant end at the bottom
- Align top of seal (Bottom of RBCs at line 0)
- Move tube holder across until top of plasma is lined up with 100% line
- Move adjustable PCV reading line to intersect with top of RBC layer (horizontal white line)
- Reccord as percentage
What colour of micohaematocrit tube is most suitable for use with an EDTA blood sample?
Blue, as it’s plain!
- As EDTA has an anticoagulant, LH in it!
- So will destroy the sample if another anticoagulant is used!
Name 4 Coagulation tests!
- BMBT (Buccal Mucosal Bleeding Time)
- ACT (Activated Clotting Time)
- Thrombocyte count
- PT (Prothrombin time)
PT can also be used alongside aPTT = activiated partial thromboplastin time
What 2 scenarios should you use a Romanowsky stain for?
+
Name 3 types!
Scenarios
1. When performing a WBC count
2. Detect blood parasites (i.e Babesia)
3 Types
1. Diff-Quick
2. Leishmann’s
3. Giemsa
What 2 scenarios should you use a Supravital stain for?
+
What is it’s other name?
Scenarios
1. Reticulocyte counts
2. To detect Heinz bodies
Other name
1. Methylene blue
Name the order in which you use these for staining a blood smear!
BRP!
- Blue (Methanol)
- Red (Eosin)
- Purple (Methylene blue)
How many times should you dip your blood smear into these stains?
+
For how long?
- Dip 5 times per stain
- Dip into stain for a length of 1 second each
What should you rinse your blood smear stain with after using completing the Romanowsky stain procedure?
+
How should you dry it?
- Rinse with Distilled water
- Place vertically + leave to dry
How would you prepare these samples to send them to an external lab?
- PPE
- Check adequate Formalin (preservative)
- Labeled with px name, species, breed, age, sex, owner’s name + date
- Ensure airtight, moisture-proof + robust
- Ensure total vol/mass is not more than 50ml/50g
- Wrap container in absorbent material
- Wrap in bubble wrap
- Place in secondary layer (plastic biohazard specimen bag) + expel any air + secure
- Complete lab paperwork + place in biohazard bag’s seperate compartment
- Identify w/waterproof marker
- Put sender’s name + address on outer packaging
- Ensure package states nature of sample + special instructions (Biohazard, handle with care)
- Send
What does increased BUN indicate in an adult dog?
- Infection
- Necrosis
- High-protein diet
- CHF *
- Urethral obstruction
- Renal failure
- Dehydration
- Systemic + metabolic conditions
- Corticosteroid therapy
- = Poor renal perfusion > less urea taken to kidneys > greater ax in blood
What 5 things may a decreased TP indicate?
- Renal disease
- Malnutrition
- Haemorrhage
- Malabsorption
- Hepatic + Pancreatic insufficiency
What are these utilised for?
To measure:
1. TP/S
2. USG
What is the normal USG range for a Cat?
1.035 - 1.60
What is the normal USG range for a Dog?
1.015 - 1.045
What is this called?
+
What chemical parameters can be assessed using it?
- Called a ‘Mutlistix’
- Can identify:
* Glucose
* Ketones
* USG
* Blood
* pH
* Protein
* Nitrates
* Leucocytes
What stain do you need for a microscopic analysis of a urine sample?
+
How many drops should you apply?
- Sedi-stain!
- 1 - 2 drops
What portion of a urine sample should you remove before adding the sedi-stain?
Supernatant, to leave a few drops to resuspend the sediment!
What test should you re-suspend the sediment in, by flicking the base of the tube?
Urine sample
What angle should you apply cover-slips onto microscope slides?
+
Why?
- 45 degrees
- To avoid air bubbles
What magnification should you use on the microscope to view a urine sample?
x10
What is most commonly found crystal in urine called?
Calcium Oxalate Dihydrate
What urinary crystal is this?
Uric acid
How many eggs per day does this louse lay for 30 days?
+
Name this louse!
- Several eggs p/day
- Trichodectes canis (Dog chewing louse)
How long does it take for this parasite’s eggs take to hatch?
7 - 14 days
How long does it take for these nymphs become reproductive adults?
2 weeks
How long is the egg > adult life cycle of Trichodectes canis?
30 - 40 days
How long can Linognathus setosus last off its host for?
No more than a few days
A dog is presented to the VP with a Dog-sucking-louse infestation.
How often should lice control treatments be repeated for?
7 - 10 days after the 1st tx
What are the eggs of this parasite called?
+
Where do they reside on the host?
- Nits
- Hair near skin
How long is the entire life-cycle of Linognathus setosus?
3 - 4 weeks
How long does it take for eggs to hatch + become nymphs in Felicola subrostratus?
1 - 2 weeks
True or False.
Lice are wingless
True
True or False.
Deep skin scrapes are the best method of dx Sarcoptes scabiei
True