Exam 3 Study Guide Flashcards
Are electron donors oxidized or reduced?
Reduced
Are electron acceptors oxidized or reduced?
Oxidized (___ate, ex sulfate)
Can electron donors donate or receive electrons?
Donate
Can electron acceptors donate or receive electrons?
Receive
What are some examples of electron donors (organic and inorganic)?
NADH/NADPH as organic examples
H2S, H2, NH3 as inorganic examples
What are some examples of electron acceptors?
Sulfate - nitrate
O2 - pyruvate
What happens when an electron donor donates their elctron?
Becomes oxidized (loses electron)
What happens when an electron acceptor receives their electron?
Becomes reduced (received electron)
Where are the electron donors located in an ETS (electron transport system)?
Start of electron transport chain
Where are the electron acceptors located in an ETS (electron transport system)?
End of electron transport chain
What is the carbon source of a fermenting cell?
Glucose (6C)
What is the energy source of a fermenting cell?
Chemical
What is the electron source of a fermenting cell?
NADH
What is the path+products of fermentation in a cell?
Glucose (6C) –EMP and/or ED–> 2 pyruvate (6C)
OR
NADH/NADPH + ATP –electron–> 2 pyruvate (6C)
2 pyruvate —-> fermentation products
What are the organisms that can ferment/oxidize substances in their cells?
Chemotroph
Organotroph
Heterotroph
What is the carbon source in a respiring cell?
Glucose (6C)
What is the electron source in a respiring cell?
NADH
What is the energy source in a respiring cell?
Chemical
What is the path+products of a respiring cell?
Glucose (6C) –EMP/ED OR NADH/NAPH+ATP –> 2 pyruvate
2 pyruvate (2*3C) –> PDC (acetyl coA + CO2 + NADH)
PDC –> TCA (CO2 –> NADH + ATP)
TCA –> ETS (recycle NADH/NADH to oxidized form)
Which step of respiration produces a lot of NADH?
TCA
Which step of respiration recycle NADH?
ETS
3 components of ETS (electron transport chain)
1) initial substrate oxidoreductase (remove electron from donor)
2) mobile electron carrier (carry electron)
3) terminal oxidase (accepts electron)
Why must the ETS be embedded in the cell membrane?
To keep ion gradient separated (H+ and PMF)
What happens during ETS operation?
1) recycles donor (NADH–>NAD)
2) proton motive force (across membrane)
3) reduce electron acceptor
PMF (proton motive force) uses…
1) ATP production / ATP synthase / oxidative
2) coupled active transport
3) antiport
4) rotate flagella
Is fermentation or respiration more efficient?
Respiration
Does fermentation or respiration produce CO2?
Respiration
Fermentation creates fermentation products
What fermentation step is ATP produced?
SLP
What respiration step is ATP produced?
SLP and oxidative
Does fermentation or respiration produce more ATP?
Respiration
What is the electron donor for fermentation?
NADH
What is the electron donor for respiration?
NADH
What is the electron acceptor in fermentation?
Pyruvate
What is the electron acceptor in respiration?
O2
Nitrates
Sulfates
Does fermentation or respiration contain the ETS and PMF?
Respiration
Fermentation may occur in a respiring cell when…
the respiring cell uses all of the electron accepetor
Lithotrophs are also…
Autotrophs
Litotrophs and phototrophs use ____ as their carbon source
CO2
What uses H2O as the e-donor in phosphorylation?
Green plants
Cyanobacteria
Organisms that use oxygenic photosynthesis
By-product is O2
Oxygenic photosynthesis occurs in…
Green plants
Cyanobacteria
What uses H2S as the electron donor in phosphorylation?
PS1
ANoxygenic PS
What uses chlorophyll as the electron donor in phosphorylation?
PS2
What are the 3 requirements for biosynthesis anabolism to occur?
Essential elements
ATP
Reducing power (electron carrier)
What are the essential elements needed for biosynthesis? Where do you get them?
C (CO2 and organic)
N (form that is easily accessible like NH3)
CO2 fixation definition:
CO2 –> organic form
CO2 fixation enzyme:
Rubisco (calvin cycle)