Exam 3 Quiz 3

Question 1

catalase test

Answer 1

-look for relative oxygen species
-use sterile toothpick to add dot of bacteria to each dot, use two drops of hydrogen peroxide, if it bubbles then there is a positive result (has catalase present)

Question 2

reactive species

Answer 2

-created in side reactions of ETC: quinines, Fe-S, flavoprotein
-hydrogen peroxide and superoxide anion

Question 3

hydrogen peroxide counter reacts using

Answer 3

catalase

Question 4

superoxide anion counter reacts using

Answer 4

superoxide dismutase

Question 5

oxidase test

Answer 5

-looking for ETC composition
-looking for cytochrome C
-use tetramethyl-p-phenylenediamine

Question 6

cytochrome C

Answer 6

peripheral membrane protein

Question 7

tetramethyl-p-phenylenediamine in reduced state

Answer 7

-e- rich
-clear coloration

Question 8

tetramethyl-p-phenylenediamine in oxidized state

Answer 8

-e- poor
-changes color to dark blue/purple/black color
-positive result, gives e- to cytochrome c

Question 9

API trips

Answer 9

-a miniature, standardized group of biochemical tests
-21 total tests, only 20 in strip (missing oxidase test)
-mostly geared against enterobcteriacae

Question 10

what media is used for API strips

Answer 10

dehydrated media, broth culture, inoculate and rehydrate at same time

Question 11

results of api strip

Answer 11

give seven digit code

Question 12

indole test

Answer 12

-SIM media
-use 5-7 drops of kovacs reagent
-red ring gives positive result
-wait ~1 min to see results

Question 13

MR-VP broth

Answer 13

transfer 3 mLs into small tube to use for voges proskaur, remainder for methyl red

Question 14

methyl red

Answer 14

-add 4-5 drops oh methyl red pH indicator
-red shows positive redult

Question 15

voges proskaur

Answer 15

-use barritts reagent
-A first (alpha napthol) 15 drops
-B second (KOH) 5 drops
-shake for 30 secs
-get result within 10-20 mins
-positive result is red

Question 16

nutrient agar

Answer 16

-control
-starting material for instantaneous test

Question 17

EMV plate

Answer 17

-selective: Gram - enteric (eosin and methyline blue)
-differential: lactose and sucrose

Question 18

both suagrs

Answer 18

high fermentation, dark purple w green metallic sheen

Question 19

one sugar ferments

Answer 19

light pink color

Question 20

no sugar ferments

Answer 20

no change

Question 21

MSA plate

Answer 21

-selective gram - staphylococcus
-differential: mannitol fermentation, yellow halo

Question 22

pathogenic MSA plate

Answer 22

aures, yellow halo

Question 23

nonpathogenic MSA plate

Answer 23

epidermidis no halo