Exam 2 Quiz 2

Question 1

Simple stain

Answer 1

involves the use of one stain to determine morphological characteristics like shape and arrangement

Question 2

stain

Answer 2

dye that is a salt that has a positive and a negative end

Question 3

positive end of a salt

Answer 3

chromophore can attach which leads to a positive or BASIC stain

Question 4

negative end of slat

Answer 4

chromophore can attach which leads to a negative or ACIDIC stain

Question 5

what is the charge of bacteria

Answer 5

negative charge

Question 6

bacilli

Answer 6

rod

Question 7

cocci

Answer 7

round

Question 8

do bacilli or cocci have more arrangement options

Answer 8

cocci

Question 9

what gives gram (-) bacteria a negative charge

Answer 9

LPS

Question 10

what give Gram (+) a negative charge

Answer 10

teichoic acid or lipoteichoic acid

Question 11

basic stain

Answer 11

-chromophore on positive end
-colored bacteria on a light background
-uses methylene crystal violet or safranin
-requires the bacteria to be fixed onto the slide through heat fixation

Question 12

acidic stain

Answer 12

-chromophore on negative end
-nigrosin
-light colored bacteria with dark background
-drop of nigrosin then bacteria and use another slide to swipe
-no fixation required
-look at slide immediately so it doesn’t crack

Question 13

is basic or acidic stian better

Answer 13

acidic is better becasue its easier to see contrast and there is no fixation required

Question 14

differential stain

Answer 14

-a Gram stain
-involves the use of two contrasting stains which allow bacteria to be separated into groups