Exam 3 Flashcards

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1
Q

An enveloped virus

A

Contains an outer membrane shell

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2
Q

Metagenomics is the study of

A

The entire genetic content of a complex microbial community

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3
Q

The capsid of a virus is

A

The protein shell surrounding the genome

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4
Q

Bacteria utilize quorum sensing to

A

identify the number of its own species in the environment

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5
Q

How many reading frames does a CHROMOSOME have?

A

6

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6
Q

What is not a mutagen?

A

DNA gyrase

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7
Q

A small circular genetic element that generally does not carry essential genes is called?

A

A plasmid

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8
Q
Which is NOT a classification of viruses?
Host
Genome
Structure
Release mechanism
A

Release mechanism

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9
Q

Transformation

A

The uptake of DNA from the environment through competence

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10
Q

Genome

A

The entire complement of genetic information of an organism

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11
Q

Proteome

A

The entire complement of protein within a cell

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12
Q

Metabolome

A

The small molecule content of a cell

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13
Q

Assembly

A

The process of alignment and connection of all of the sequenced fragments of a genome

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14
Q

Transduction

A

The transfer of bacterial chromosomal DNA from one cell to another via bacteriophage

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15
Q

Conjugation

A

The transfer of DNA from one cell to another via sex pilus

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16
Q

Transcriptome

A

The entire complement of RNAs within a cell

17
Q

Transposons

A

Discrete segments of DNA bracketed by inverted repeats that hop between DNA molecules

18
Q

Annotation

A

The process of converting the raw DNA sequence to a list of gene

19
Q

Three types of viral infections

A

Acute, persistent, latent

20
Q

Two types of transposition

A

Conservative and replication

21
Q

Direct Reversal

A

Mutated base is still recognizable and can be repaired without referring to other strands

22
Q

Nucleotide Excision Repair

A

Damaged DNA is removed and repaired using opposite strand as template

23
Q

Recombination

A

Physical exchange of DNA between genetic elements in order to by pass damage

24
Q

How do you identify where open reading frames are located?

A
  1. Find the start codon
  2. Find the termination codon
  3. Calculate the size of the potential open reading frame-usually 1000 nucleotides long
  4. Identify the ribosomal binding site
  5. Codon bias
25
Q

Horizontal gene transfer

A

Through conjugation (Sex pilus, transformation (Competence) and transduction (Transfer chromosomal DNA using bacteriophage)

26
Q

Vertical gene transfer

A

Daughter cells through binary fission, same genes throughout

27
Q

Describe a mechanism that bacterial cells can protect themselves from

A

Process of CRISPR; Foreign DNA is injected into the cell. DNA has spacers put on to mark it as foreign. DNA is incorporated into bacterial DNA and labeled as viral DNA. Just has to survive the first invasion

28
Q

Which is not a hypothesis for the origin of viruses?
Arose from parasitic cells, eventually shedding most of its genome
Arose from DNA or RNA that escaped from the chromosome of a cell
Arose separately from the first cells and from very different complex organic molecules
Arose separately from the first cells but from the same complex organic molecules

A

Arose separately from the first cells and from very different complex organic molecules

29
Q
Which is not a mutagen?
Nucleotide base analogs
Intercalating agents
Non-ionizing radiation
DNA gyrase
A

DNA gyrase

30
Q

A small circular genetic element that generally does not carry essential genes is called

A

A plasmid