Exam 3 Flashcards
Translucent film composed of glycoproteins from saliva
Acquired pellicle
How long does it take for acquired pellicle to form after teeth are cleaned with abrasive agent?
30-90 minutes
How can pellicles be removed?
- Brushing with abrasive toothpaste
- Whitening products
- Acidic food & drink
What is the first step in soft deposit formation?
Acquired pellicle
What protects teeth from acids that can cause demineralization of the enamel surface?
Soft deposits
What are the significants of soft deposits?
-Protection
-Lubrication
-Nidus for bacteria
-Provides attachment for biofilm & calculus
What pellicle is translucent and may pick up stains?
Acquired enamel pellicle
Non-mineralized community of microorganisms
Dental biofilm (plaque)
What will decay faster than an enamel surface?
A root surface covered in dentin
What is the first step in dental biofilm?
Pellicle formation
What is the second step in dental biofilm?
Initial adhesion; planktonic bacteria attach
What is the third step in dental biofilm?
Maturation
What is the fourth step in dental biofilm?
Detachment & dispersion
What provides the mechanism for adhesion of bacteria?
Glycoproteins from pellicle
compounds secreted by microorganisms that form a matrix around microcolonies
Extracellular polymetric substance (EPS)
Holds bacteria together
Co-adhesion
What helps with adhesion and attachment of biofilm to teeth?
Stickiness
What protects biofilm?
Antimicrobial enzymes
Early colonizing bacteria provide a source of what?
nutrients for late colonizing bacteria
What is Quorum sensing?
cell-to-cell communication
What days for Leo’s gingivitis man study is this?
- Early biofilm consists primarily of gram-positive cocci with small accumulations of leukocytes
Day 1-2
What days for Leo’s gingivitis man study is this?
- The cocci still dominate while increasing numbers of gram-positive filamentous form and slender rods join the surface of the cocci colonies, along with more leukocytes
Day 2-4
What days for Leo’s gingivitis man study is this?
- Filaments increase in numbers, and a mixed flora appears compromising rods, filamentous forms, and fusobacteria with heavy accumulations of leukocytes
Day 5-10
In what days is gingivitis clinically evident?
Day 10-21
Greater variability in architecture than subgingival biofilm; two layers of mainly gram-positive, aerobic bacteria
Supragingival biofilm
Basal layer adheres to the tooth
The second layer adheres to the top of the basal layer
Supragingival biofilm
Architecture is predominately gram-negative, anaerobic, and motile
Changes in health, gingivitis, and disease
Subgingival biofilm
Organic & inorganic solids = what % of biofilm composition?
20%
Water = what % of biofilm composition?
80%
What are the inorganic elements of biofilm?
Calcium
Phosphorus
Fluoride
Polysaccharides metabolized by S. mutans to produce glucans & fructans
Proteins bind with glucans to support biofilm growth
Organic elements of biofilm
a shift of healthy species to periodontitis-associated species
Dysbiosis
What factors influence biofilm formation?
Location
Character of tooth surface
Malocclusion
Personal hygiene
How can you detect plaque?
Direct vision
Explorer
Disclosing solution
What is disclosing solution used for?
- Identity biofilm not visible
- Evaluate patient’s ability to rinse
Spit out
Expectorate
o Usually from general neglect
o Loosely adherent mass of bacteria & cellular debris
o Bulky & soft resembles cottage cheese
o Forms over dental biofilm
o Product of living and dead bacteria, leukocytes, salivary proteins and food debris
o Can be removed by rinsing, water spray, or tongue action
Materia alba
Where can food debris be found?
- Cervical third
- Embrasure and proximal spaces
- Open contacts
- Orthodontic appliances
- Bridgework
biofilm mineralized by crystals of calcium phosphate mineral salts
Calculus
What are the parts of calculus composition?
Tarter
Inorganic content
What are the major inorganic components of calculus?
Calcium
Phosphorus
Carbonate
Sodium
Magnesium
What are the crystals in calculus made from?
Brushite
Hydroxyapatite
Whitlockite
Octocalcium Phosphate
What are the organic components of crystals?
- Various microorganisms
- Desquamated epithelial cells
- Leukocytes
- Saliva
What calculus is easy to remove?
Acquired pellicle
What is a breeding ground for more biofilm?
calculus
What does calculus act as a reservoir for?
Bacteria
Endotoxins
What is a secondary etiology factor in periodontitis?
Calculus
What are clinical characteristics of calculus?
color
shape
consistency & texture
size & quantity
distribution
What are the colors of supragingival calculus?
White, creamy, yellow, or gray
What is the shape of supragingival calculus?
Amorphous
Bulky
What is the consistency & texture of supragingival calculus?
Moderately hard, covered with nonmineralized biofilm
What are the colors of subgingival calculus?
Light to dark brown
Dark green
Black
What is the shape of subgingival calculus?
Conforms to root surface
What is the consistency & texture of subgingival calculus?
Harder & more mineralized, surface covered with biofilm
What is the size & quantity of subgingival calculus?
-Personal oral care
-Age
-Bacterial load
-Disease severity
What is the size & quantity of supragingival calculus?
-Personal oral care
-Diet
-Salivary flow
-Position of teeth
-Use of tobacco
What is the source of minerals for supragingival calculus?
Saliva
What is the source of minerals for subgingival calculus?
Gingival sulcular fluid