Exam 2 Flashcards
Mitosis
a type of cell division by which a diploid somatic cell duplicates its DNA and divides once to produce two genetically identical diploid daughter cells.
multicellular organisms use this type of cell division for tissue growth and repair
Mitosis
Diploid (2N) cells
contain two complete sets of homologous chromosomes, which have the same size, the same shape, and carry genes for the same traits.
In order for a eukaryotic cell to reproduce itself
it must duplicate its DNA and distribute that DNA to the resulting daughter cells.
Human somatic cells contain
23 pairs of homologous chromosomes
The cell cycle
an orderly sequence of events that describes the life of a cell.
occurs between successive divisions of a cell and is the longest portion of the cell cycle.
Interphase
the first “Gap” phase (G1)
a cell duplicates its organelles, synthesizes proteins, grows, and carries out its metabolic activities
how long do Typical somatic cells spend in G1
8-10 hours
the “Synthesis” phase (S)
the DNA molecules are replicated in order to produce two identical copies of the DNA
how long do Typical somatic cells spend in S
6-8 hours
the second “Gap” phase (G2)
cell growth continues, proteins that are needed for cell division get synthesized, and centrioles are replicated
how long do Typical somatic cells spend in G2
4-6 hours
describes division of the nucleus, which is followed by division of the cytoplasm.
Mitosis
how long do Typical somatic cells spend in Mitosis
1-2 hours
The length of the cell cycle varies from one type of cell to another.
Some cells (skin) will divide continuously Some cells (liver) divide very slowly unless organ is damaged If damaged, cells divide more rapidly Some cells (nerve cells, heart muscle) lose their ability to divide and get replaced by scar tissue when they die
How many stages are required to divide the nucleus in mitosis
five sequential stages
PROPHASE
The first stage; chromatin in the nucleus condenses into thick, rod-shaped chromosomes
Each chromosome consists of identical pair of chromatids joined together at centromere
the nuclear membrane starts to disintegrate
nucleoli disappear
pairs of centrioles migrate toward opposite poles of the cell
Mitotic spindle
PROMETAPHASE.
The second stage; microtubules form a spindle apparatus with fibers that attach one identical chromatid to one pole of the cell and the other identical chromatid to the opposite pole of the cell
chromosomes migrate toward the center of the cell as pairs of chromatids
METAPHASE
The third stage; chromosomes line up randomly along a metaphase plate at the center of the cell with one chromatid located on each side of the cell’s “equator”
ANAPHASE
The fourth stage; centromeres split and microtubules pull the identical chromatids apart
chromatids migrate toward opposite poles of the cell as they become daughter chromosomes
Only use term chromatid when attached by centromere, once separate they are daughter chromosomes
TELOPHASE.
The final stage; daughter chromosomes decondense and form a tangled mass of chromatin
a new nuclear envelope forms around each tangled mass of chromatin
the spindle apparatus is disassembled
nucleoli reappear
Cytokinesis
begins during late anaphase or early telophase as the cytoplasm divides.
A variety of factors are needed to initiate cell division.
sufficient cytoplasm must exist to be distributed to the daughter cells
DNA replication must be completed
an adequate supply of nutrients must be available to the cell
maturation promoting factor, cdc2 proteins, and cyclin must be present
adjacent cells die and create space for new cells to occupy
Certain factors can halt cell division.
withdrawal of nutrients or growth factors
contact inhibition blocks cell division when cells touch one another
apoptosis is the orderly, genetically programmed death of a cell
Meiosis
another type of cell division by which a diploid germ cell duplicates its DNA and divides twice to produce four genetically different haploid gametes.
germ cells
special reproductive cells in the gonads that produce eggs or sperm
Haploid (N) cells
contains only one complete set of chromosomes.
Meiosis requires ___ nuclear divisions to separate and distribute the DNA into gametes.
two
the first nuclear division (Meiosis I)
a reduction division because number of chromosomes gets reduced from diploid (2N) to haploid (N)
the second nuclear division (Meiosos II)
an equatorial division; chromatids that comprise a chromosome get separated and distributed to different gametes
prophase I
DNA condenses into visible chromosomes.
homologous chromosomes pair up to form a tetrad
maternal and paternal chromatids within a tetrad exchange pieces of DNA during crossing-over
Produces new gene combinations on chromatid
nuclear envelope disintegrates
nucleoli disappear
centrioles assemble microtubules into a spindle apparatus
metaphase I
tetrads line up at the center of the cell.
pair of chromatids that make up each homologous chromosome lie on opposite sides of the cell’s equator
anaphase I
tetrads separate and the two chromatids that make up each homologous chromosome move toward opposite poles of the cell.
telophase I
the cytoplasm gets divided and two haploid cells result.
unpaired chromosomes decondense into a tangled mass of chromatin
a new nuclear envelope forms around each tangled mass of chromatin
the spindle apparatus is disassembled
nucleoli reappear
Interkinesis
a quiet phase, during which time each haploid daughter cell prepares to divide again.
Major difference between meiosis II and Mitosis
meiosis II involves haploid cells, while mitosis involves diploid cells
The second nuclear division of meiosis II
similar to mitosis because the pairs of non-identical chromatids are separated and distributed into four gametes.
The first meiotic division reduces ….
the number of chromosomes from 46 in the diploid germ cell to 23 in each haploid daughter cell.
The second meiotic division separates …
each of the 23 pairs of chromatids in each daughter cell and distributes them into a pair of gametes.
Cancer
refers to any disease characterized by uncontrolled cell division that produces abnormal tumors or neoplasms.
benign tumors
tend to grow slowly and don’t spread to other organs, but they interfere with normal body function and/or appearance
malignant tumors
non-encapsulated, they grow rapidly, and they spread to other organs by way of blood or lymph
do not exhibit contact inhibition and easily break loose from tumor to ‘seed’ growth of new tumors
Malignant cells
enclosed within fibrous capsule
Most benign tumors
Contact inhibition
cells come into contact with another cell and they stop cell division temporarily
Metastasis
describes the spread of cancerous cells to other parts of the body.
the initial invasion of malignant cells into the surrounding tissue
produces a primary tumor that competes for space and nutrients and ultimately destroys normal tissue
secondary tumors
malignant cells may detach from this primary tumor and establish secondary tumors with new networks of blood vessels that provide nutrients for rapid growth
approximately _____ of cancer deaths result from metastasis
90%
how is cancer named
according to the cells or tissues from which the primary tumor arises.
carcinomas
arise from epithelial cells
melanomas
arise from pigment-producing melanocytes in the skin
sarcomas
arise from bone cells or muscle cells
leukemia
arises from abnormal white blood cells produced in the bone marrow
lymphomas
arise from lymph nodes
90% of all cancers are
carcinomas
______ of cancers are caused by environmental carcinogens that cause mutations.
60% to 70%
oncogenes
mutant forms of normal genes called proto-oncogenes
code for proteins that stimulate cell division, cell growth, cell adhesion
Proto- oncogenes
produce excess growth- factor or abnormal produce excess growth- factor or abnormal growth-factor receptors that stimulate continual cell division
Oncogenes
tumor suppressor genes
inhibit cell division or inactivate carcinogens
Loss of, or damage to, tumor suppressor genes…
can trigger cancer
the “accelerator” of the cell cycle
oncogenes
the “brakes” on the cell cycle
tumor suppressor genes
most cancers are treated by
surgical removal of the malignancy
chemotherapy
administers drugs that poison malignant cells
radiation therapy
destroys the chromosomes in malignant cells in order to prevent cell division
malignant cells are vulnerable to …
chemotherapy and radiation therapy because they divide faster than normal cells
treatment is often difficult because…
the variety of cells in a tumor may respond differently to different chemicals and radiation
DNA is composed of
two long chains of deoxynucleotides
each deoxynucleotide contains
the sugar deoxyribose, which has a phosphate group attached to the 5’ carbon and a nitrogenous base attached to the 1’ carbon
Nitrogenous bases of DNA
adenine, guanine, cytosine, and thymine
The DNA molecule takes the shape of
a double helix.
‘side of the ladder’
the sugar of one deoxynucleotide is linked to the phosphate group of the next deoxynucleotide by a covalent bond, which creates sugar phosphate chain
‘rungs of the ladder’
complementary base pairs are held together by hydrogen bonds
The two chains that make up the DNA double helix are
antiparallel
Unwinding
an enzyme breaks the hydrogen bonds that hold complementary base pairs together ‘unzipping’ DNA double helix
Pairing
free deoxynucleotides in the nucleoplasm form hydrogen bonds with deoxynucleotides on each template to create complementary base pairs
Joining
deoxynucleotides attached to the template are joined together by covalent bonds to form a “daughter” strand that is complementary to its “parent” strand template
DNA replication is semiconservative
because each replicated DNA molecule consists of one original “parent” strand plus one newly synthesized complementary “daughter” strand.
why are Errors in DNA replication are quite rare
because enzymes “proofread” the daughter strand and correct mistakes in complementary base pairing.
mutations
Uncorrected errors that are passed on to next generation
RNA is composed of
one chain of ribonucleotides.
each ribonucleotide contains
the sugar ribose, which has a phosphate group attached to the 5’ carbon and a nitrogenous base attached to the 1’ carbon
Nitrogenous bases of RNA
adenine, guanine, cytosine, and uracil
RNA can exist as
a long, single strand or a twisted structure with hairpin loops.
messenger RNA (mRNA)
carries coded genetic information from the DNA in the nucleus to ribosomes in the cytoplasm
ribosomal RNA (rRNA)
holds ribosome subunits together during protein synthesis
transfer RNA (tRNA)
) binds to a specific amino acid in the cytoplasm and delivers it to a ribosome to be incorporated into a growing polypeptide
transcription
the process by which genetic information that is stored in DNA is copied into a strand of messenger RNA
the sense strand
its sequence of deoxynucleotides contains coded information that “makes sense” for synthesizing a protein
the template strand,
it will be transcribed into molecule of messenger RNA
pre- mRNA
contains exons that code for segment of a protein and introns that do not code for any part of protein
Introns
must be removed from pre-mRNA and exons must be spliced together to produce functional mRNA molecule
After transcription is completed,
the two strands of DNA are rejoined and the mRNA passes out of the nucleus and into the cytoplasm where translation will occur.
must be linked together in the correct sequence to synthesize a protein.
Amino acids
codon
Three consecutive ribonucleotides in mRNA
the four ribonucleotides that make up mRNA must somehow specify____ different amino acids that are used in protein synthesis
20
there are three “stop codons” that halt protein synthesis
UAA, UAG, UGA
Translation
the process by which the genetic information that was coded into mRNA gets converted into a protein.
bind to mRNA and search for the start codon (AUG) in order for translation to begin.
The two ribosome subunits
Additional tRNA molecules
bring their amino acids to the ribosome according to the codons in the mRNA strand.
Gregor Mendel
Our understanding of heredity comes from his experiments
why did Mendel chose to study garden peas
because they were easy to grow, they expressed distinct traits without intermediate effects, and he could control the breeding.
pure-breeding
peas that are self-pollinated produce offspring with same traits as the parents
hybrids
peas that are cross-pollinated produce offspring with a mixture of parental traits
Law of Segregation
each organism contains two “factors” for any given trait and these factors separate during gamete formation, so each gamete contains only one factor for each trait
Law of Independent Assortment
members of one pair of factors separate independently of members of another pair of factors so all possible combinations of factors can occur in the gametes
a gene
a segment of DNA on a chromosome that controls the expression of structural, physical, or even behavioral traits
locus
The location of a particular gene on a chromosome
contain both members of a pair of homologous chromosomes, so they possess two copies of any particular gene.
Diploid cells
contain only one member of a pair of homologous chromosomes, so they possess only one copy of any particular gene
haploid cells
alleles
Alternate or contrasting forms of a gene
genotype
The combination of the two alleles for a particular trait
if the same allele is received from each parent, the genotype is ______________ and organism is _______________
homozygous; pure-breeding
if contrasting alleles are received from the parents, the genotype is _______________ and organism is _____________
heterozygous; hybrid
phenotype
Only one allele is usually expressed at any time and the physical expression of the genotype
dominant allele
the allele that is expressed when two contrasting forms of a gene are present at the same time
recessive allele
the allele that is not expressed when two contrasting forms of a gene are present at the same time