exam 1 Flashcards
what is DNA?
material of inheritance that is common to all organisms
what is evidence that all organisms evolved from a single common ancestor?
DNA is common to all organisms
what is the most accurate measure of relatedness?
how similar genomes are
what was the first genetic material that was widely compared?
rRNA sequences
life depends on ______ synthesis.
protein
what functions to make proteins?
ribosomes
genes for rRNA evolve _________.
very slowly
why were rRNA the first widely compared sequences?
the length of the sequences are ideal and they evolve very slowly
_____ confirmed many previous classification schemes.
sequencing
what is archaea more closely related to?
eukaryotes
what are eukaryotes?
cells with a nucleus
what are prokaryotes?
cells without a nucleus
archaea were originally thought to be what?
a type of bacteria
what are the 3 domains of life?
bacteria, archaea, and eukarya
why are dyes used to visualize bacteria?
bacteria are hard to see even at 100X
what is gram staining?
using violet, then red dye
what does gram positive mean?
the cell wall of a bacteria takes up the violet dye (and looks purple)
what does gram negative mean?
the cell wall of a bacteria is underneath an outer membrane (and appears pink from the red counterstain)
what is (and isn’t) gram staining useful for?
useful for identification, but not for phylogenies
how does gram staining work?
the purple dye sticks to the peptidoglycan in the cell wall when it is on the outside of the cell, but not when it is between layers of membranes
where are prokaryotes found?
everywhere on earth, even in extreme environments
what are the 3 shapes of bacteria?
coccus (spheres), bacillus (rods), and helix (spiral)
what are ways that bacteria can move?
by using flagella, twisting, or gliding (and some don’t move)
how do bacteria communicate?
by chemicals or light
what is quorum sensing?
bacteria monitor the amount of their specific species of bacteria there are, and can tell when there is a minimum amount present to form a biofilm
what is a biofilm formation?
aggregation of bacteria to protect the bacteria (hard for antibiotics to work)
prokaryotes are often ____cellular.
uni
even though prokaryotes are often single celled, they often live in _____.
colonies
how do prokaryotes reproduce?
fission (asexual reproduction)
what is horizontal/lateral gene transfer?
genetic material moves between organisms, not through generations
what is vertical gene transfer?
genetic material moves generation to generation, like in multicellular organisms
what is DNA transfer by conjugation?
DNA (a plasmid) from a living donor cell is transferred to a recipient cell via a pilus
what is DNA transfer by transformation?
DNA is released into the environment by a dead cell, and is then taken up by a living recipient cell
what is DNA transfer by transduction?
DNA is transferred from a donor cell to a recipient cell via a virus
what effect does lateral gene transfer have on phylogenies?
it complicates them
True or False: all prokaryotes require oxygen for survival.
False- some live in oxygen-free environments
what are obligate anaerobes?
organisms that must be in environments without oxygen
what are obligate aerobes?
organisms that must be in environments with oxygen
what are facultative anaerobes?
organisms that can be in environments that do or do not have oxygen
what are photoautotrophs?
organisms that feed themselves using light
what are photoheterotrophs?
organisms that harvest energy from light but rely on sugars made by organisms for food
what are chemoheterotrophs?
organisms that feed on other organisms
what are chloroplasts evolved from?
symbiotic cyanobacteria
what type of organisms was responsible for converting Earth’s atmosphere to aerobic in the ancient past?
photoautotrophs
what are decomposers?
organisms that break down dead bodies- they return carbon to the soil and atmosphere
what are nitrogen fixers?
organisms that convert N2 from the atmosphere into NH3 (ammonia)
what are nitrifying bacteria?
NH3 -> NO2 -> NO3
what is denitrifying?
organic nitrogen is converted to N2
True or False: all prokaryotes are harmful in the form of pathogens.
False- some are beneficial, and some are neutral, and some are unknown
what is an example of beneficial prokaryotes?
legume roots house nitrogen-fixing bacteria
what is an example of harmful prokaryotes?
agrobacterium in plants that causes crown galls
the human body harbors ____ bacterial cells as human cells.
4X
Only a tiny percentage of prokaryotes are ________.
pathogens
how do pathogens cause disease?
invade the body, evade the immune system, multiply, and produce toxins
what are endotoxins?
they expose the host to the pathogen when bacteria lyse (when the immune system kills the bacteria)
what are exotoxins?
they are released by living bacteria (an active pathogen- the immune system can’t fight it well)
which form of toxin can the human body tolerate more?
endotoxins
what are some symptoms of endotoxins?
fever, vomiting
what are some symptoms of exotoxins?
they are highly poisonous or fatal
what are viruses?
small molecules composed of protein-coated DNA or RNA, encoding a small number of proteins
what is the best evidence that viruses are not alive?
they are acellular
though viruses are not alive, they ______
mutate, evolve, interact with other organisms, and are derived from other organisms
how are viruses classified?
based on nucleic acid type (DNA or RNA)
what are positive sense RNA viruses?
the genome is also a template for translation
what are some examples of positive sense RNA viruses?
rhinovirus, polio, covid
what are negative sense RNA viruses?
the complement to the genome is the template for translation
what is RNA-dependent RNA polymerase?
it makes the complementary strand upon infection, which is the template for translation
what are examples of negative sense RNA viruses?
rabies, measles, mumps
what is an RNA retrovirus?
RNA is used to make a DNA copy (reverse of the normal flow of info) via reverse transcriptase, then pro-viral DNA is integrated into the host’s genome
which virus type is specific to vertebrate animals?
RNA retrovirus
what is an example of an RNA retrovirus?
HIV
what are examples of DNA viruses?
smallpox, herpes, and a bacteriophage
what are main features of eukaryotes?
they have an organized nucleus (with linear chromosomes and a membrane), and contain organelles
what are organelles?
membranes associated with specialized functions
what do vesicles do?
move materials in and out of the cell
what does cellular movement in eukaryotes rely on?
predation, changing shapes, and an increase in size
which organelles are believed to have originated via endosymbiosis?
chloroplasts and mitochondria
eukaryotes have a _______ relationship with prokaryotes.
symbiotic
what is characteristic of eukaryotic DNA molecules?
they are linear with multiple origins of replication and carry many genes and regulatory regions
all eukaryotes have a ______ life cycle.
sexual
gametes have _____ the normal chromosome number because of meiosis.
1/2
what is the fusion of gametes called?
fertilization
what increases diversity?
sex
what produces variations in life cycles?
haploid and diploid phases can be persistent or brief that depend on timing of meiosis and fertilization
what are large eukaryotes?
plants, animals, and fungi
what are small eukaryotes?
protists (usually single-celled)
multicellularity allows for what?
specialization (increased efficiency), greater size, and mortality
what are the 3 main superkingdoms?
plants, animals and fungi, and protists
characteristics of opisthokonts
single flagellum on the back end of the cell
what organisms are opisthokonts?
animals, fungi, and choanoflagellates
opisthokonts are _____cellular
uni and multi-
what are choanoflagellates?
cells that can be colonial, which can be believed to be the first step toward multicellularity
what could be considered to be the ancestor to animals?
choanoflagellates
what are main characteristics of fungi?
cell wall made of chitin, absorptive heterotrophy (they digest food in the environment then reabsorb the molecules it needs for cellular respiration), feeding on living or dead organisms
why are fungal infections in animals difficult to treat?
many fungicides are toxic to animal cells because of how closely related animals and fungi are
what are single-celled fungi?
yeasts
what are hyphae?
strands of fungi together that make up mushrooms (look kind of like roots)
what are the 2 main parts of mushrooms?
underground- mycelium
aboveground- fruiting body (makes spores in gills)
what are fungal plant pathogens?
rusts and smuts
fungi can be beneficial, what is an example?
symbiosis of mycorrhizal fungi that allow trees to take up more minerals from the soil
what are lichens?
fungus + green algae or cyanobacterium
characteristics of amoebozoans
use pseudopods for motility and have a flexible membrane
what are 3 examples of amoebazoans?
amoeba, physarum, and dictyostelium
what is amoeba?
a lobosean that lives in fresh water; eats via phagocytosis and is a scavenger and predator
what is physarum?
a plasmodial slime mold found in forests; they disperse, then aggregate to form fruiting bodies and form spores by meiosis, they are scavengers that move by cytoplasmic streaming
what is dictyostelium?
a cellular slime mold with amoeba-like single cells that aggregate into a “slug” when dry or starved; the cells are retained and form spores
amoebazoans are _____cellular.
(mostly) uni
characteristics of rhizarians
unicellular aquatic organisms with long pseudopods (that look kind of like roots)
what are 2 types of rhizarians?
foraminiferans and radiolarians
what are foraminiferans?
have an external shell of calcium carbonate, and are a source of limestone when they decompose over time; pseudopods ensnare plankton for food
what are radiolarians?
have glassy endoskeletons made of silica and include some of the largest unicellular eukaryotes; can have photosynthetic endosymbionts (dinoflagellates)
characteristics of archaeplastids
land plants
what are some examples of archaeplastids?
red algae, green algae
what chlorophyll does red algae use?
chlorophyl a and phycoerythrin
what chlorophyll goes green algae use?
chlorophyl a and b
characteristics of stramenophiles?
they have unequal flagella
what are 2 types of stramenophiles?
diatoms and brown algae
what are diatoms?
single celled organisms with silica in cell walls that are aquatic and photosynthetic; filtration via diatomaceous earth
what is brown algae?
can be multicellular (sea palms and giant kelp) that are photosynthetic (chlorophyll a and c + brown pigments); source of emulsifiers of ice cream
where do chloroplasts in green algae and land plants come from?
cyanobacterium endosymbiosis
what is a clue to endosymbiosis leading to chloroplast formation?
more than one membrane surrounding the chloroplast
what is serial endosymbiosis?
more than 2 membranes around the chloroplast (derived from red algae)
characteristics of alveolates
single celled organisms, with sacs under the cell membrane
what is paramecium?
ciliate (body covered in cilia for motility) that live in fresh water
what are dinoflagellates?
have 4 membrane chloroplasts and can cause red tides (large algal growth)
what is plasmodium?
parasite that causes malaria with life cycles in mosquitoes and human blood; has nonfunctional chloroplasts
what are examples of alveolates?
paramecium, dinoflagellates, and plasmodium
characteristics of excavates
unicellular and often lacking mitochondria; named for the surface groove