Exam 1 Flashcards
What is microbiology
study of small living things
what is the working definition of microbiology
study of entities too small to be seen with the unaided human eye (<0.2mm=200micrometers=200,000nanometers)
Which of the following is cellular and which is acellular: Prokaryotes, Eukaryotes, Viruses
Cellular: Prokaryotes, Eukaryotes
Acellular: Viruses
Bacteria and archaea are ____
a) prokaryotes
b) eukaryotes
c) viruses
a) Prokaryotes
fungi, algae, protozoa, and helminths are _____
a) prokaryotes
b) eukaryotes
c) viruses
b) eukaryotes
bacteriophage are ___
a) prokaryotes
b) eukaryotes
c) viruses
c) viruses
Which of these sequences arranges the microbes from smallest to largest: 1 Escherichia coli (bacterium) 2 Paramecium caudatum (Protozoa) 3 Adenovirus (virus) 4 Candida albicans (yeast)
- Adenovirus (virus)
- Escherichia coli (bacterium)
- Candida albicans (yeast)
- Paramecium caudatum (protozoa)
What characteristics do both living organisms and viruses share?
Ability to reproduce and evolve
what are the characteristics of living organisms
metabolism, reproduction, differentiation, communication, locomotion, evolution
Which of the following are reasons it is important to study microbiology:
- Microbes are the earliest organisms found in the fossil record
- They perform essential reactions in the environment
- microbes can be harnessed to work for us
- they sometimes cause infectious diseases
all of the above are correct
What is biotechnology
altering the use of microbes to produce useful products or modify other organisms
T or F: all microbes cause infectious diseases
F: only a few percent of all microbes are associated with disease
What is bacteriology
study of prokaryotes
mycology
study of fungi
phycology
study of algae
protozoology
study of protozoa
virology
study of viruses
immunology
study of the immune system
parasitology
study of parasites and their hosts
Lipoteichoic acid is found in
a) gram positive cell
b) gram negative cell
c) endospore
a) gram positive cell
____ have membranes made of mycolic acids and arabinogalactans
a) gram positive
b) endospores
c) mycobacteria
d) gram negative
c) mycobacteria
____ have thin peptidoglycan layers covered by an outer membrane with toxigenic properties on many pathogens
a) gram negative
b) gram positive
c) mycobacteria
a) gram negative
Which of the following is NOT a component of a lipopolysaccharide
a) Lipid A (six fatty acids)
b) (adaptor structure)
c) O-antigen (repeats)
d) A-antigen (singles)
d) A-antigen (singles)
this isn’t a real thing
Which of the following is NOT true of Mycoplasma
a) true bacteria
b) ergosterol in membranes
c) very small in size
d) no peptidoglycan wall
e) variable in shape
f) are important pathogens
g) cholesterol in membranes
b) ergosterol in membranes
Which of the following is NOT true of flagellum structures in bacteria
a) bacterial locomotion
b) electric motor
c) dozens of proteins
d) rotates rapidly
e) many are reversible
f) whip back and forth
g) propels the cell through its environment
f) whip back and forth
Which of the following best describes the flagellar arrangement monotrichous
a) a single flagellum at one end
b) two flagella, one at each end
c) a clump of flagella at one end
d) flagella all around
a) a single flagellum at one end
What flagellar arrangement is two flagella, one at each end?
a) monotrichous
b) amphitrichous
c) lophotrichous
d) peritrichous
b) amphitrichous
Aristotle favored ____
a) biogenesis
b) spontaneous generation (abiogenesis)
spontaneous generation (abiogenesis)
Who used heat and chemicals to treat the incoming gas (air) for their boiled media broth?
Schulze and Schwann
Who discovered endospores
a) Pasteur
b) Redi
c) Hooke
d) Cohn
Cohn
Who used carbolic acid (phenol) to clean hands, wound, and operating rooms for application of germ theory
a) Hooke
b) Pasteur
c) Lister
c) Lister
What are the four parts of Koch’s Postulate?
- The suspected pathogenic organism should be present in all cases of disease and absent from healthy animals
- Isolate the pathogenic organism and grow in a pure culture
- Cells from the pure culture of the suspected organism should cause disease in a healthy animal
- The organism is reisolated into a pure culture that is identical to the original pure culture
Microbes belonged to which kingdom
monera
What did Woese use to classify his domains
a) DNA
b) 16S rRNA
c) mRNA
b) 16S rRNA
Which of the following contain axial filaments?
a) Spirochetes
b) bacillus
c) cocci
a) Spirochetes
What flagellar arrangement has a bundle of flagella at one end
a. monotrichous
b. amphitrichous
c. lophotrichous
d. peritirchous
c. lophotrichous
T or F: Peritrichous have flagella all around, not just at the ends
True
____ is the movement of a bacterium in response to chemical gradients and is described at “Run and Tumble”
a) whipping
b) rolling
c) chemotaxis
d) chemical shifts
c) chemotaxis
What movements is analogous to blindfolding your friend and having them walk around
a) unbiased running
b) planned walk
c) Biased random walk
d) unbiased jog
c) biased random walk
What attaches cells to surfaces
a. fimbriae
b. stalks
c. pillars
d. both a and b
both a and b, fimbriae and stalks
Most capsules/glycocalyx are made of:
a) peptides
b) carbohydrates
b) carbohydrates
very few are made of peptides
What is the ability to distinguish two adjacent objects or points from one another called
a) magnification
b) resolution
c) clarity
d) distinguishing factor
b) resolution
What is the equation for resolving power (RP)
wavelength of light in nm/ 2 times numerical aperture
T or F: shorter wavelengths produce a better resolution
True
What is a clump of 4 cells in a single layer referred to as
a. bacillus
b. tetrad
c. sarcina
d. streptococci
b. tetrad
what bacterial cell shape is shaped like a bean
a) coccus
b) bacillus
c) vibrio
c) Spirillum
c) vibrio
which of the following is NOT an advantage of small size
a) more surface area:cell volume
b) faster growth rates
c) faster evolution
d) slower evolution
d) slower evolution
T or F: all cells have a cell membrane
True - this is the structure that defines the existence of a cell
A phospholipid consists of glycerol w/ ____ links to ___ fatty acids and a phosphoryl head group
a) ether; 2
b) ester; 2
c) ether; 1
d) ester;1
b) ester; 2
T or F: bacterial membranes are 50% phospholipids and 50% proteins
True; bacterial membranes are equal parts phospholipids and proteins
Which of the following is NOT a function of a membrane
a) structural support
b) secretion of virulence factors
c) transmission of communication signals
d) ion transport and energy storage
e) appear more intimidating to other cells
e) appear more intimidating to other cells
Membranes are ____ permeable
a) fully
b) non
c) semi
c) semi
What is the purpose of the diffusion of weak acids and bases
a) carry water into the cell
b) change the structure of the cell
c) change the pH of the cell
d) protect the cell
c) change the pH of the cell
What are the two types of transport across a membrane?
Which requires transport proteins?
- Passive transport
2. Active transport - requires transport protein
____ reinforces eukaryotic membranes, ____ and _____ reinforce bacterial membranes
a) hopanoids
b) sterols
c) hopanes
c) steriods
b) sterols reinforce eukaryotic membranes
a) hopanoids and c) hopanes reinforce bacterial membranes
Bacteria are ____
a) diploid
b) haploid
b) haploid
The total size of a prokaryote ribosome is 70S, which is made of two subunits ___ and ___
a) 50S and 20S
b) 30S and 40S
c) 60S and 10S
d) 30S and 50S
d) 30S and 50S
What are extensively folded intracellular membraes called
a) inner membranes
b) endomembrane
c) envelopes
d) thylakoids
d) thylakoids
Polyhedral bodies packed w/ the enzyme Rubisco for CO2 fixation are ___
a) carboxysomes
b) oxidizing agents
c) gas vesicles
a) carboxysomes
What is the function of gas vesicles in phototrophs?
a) nutrient storage
b) transport
c) increase buoyancy
c) increase buoyancy
____ ____ are inclusions bodies that store glycogen, PHB, sulfer, volutin, and magnetosomes
storage granules
What forms a “Z ring for septum placement”
a) FtsZ
b) MreB
c) CreS
d) DeG
a) FtsZ
What forms a coil inside rod-shaped cells
a) bacillus
b) MreB
c) spirochetes
d) CreS
b) MreB (b for bacillus)
What forms a polymer along the inner side of crescent-shaped bacterial
a) cell membrane
b) cell wall
c) MreB
d) CreS
d) CreS
What does the cell wall help with aside from the shape and rigidity of the cell
a) transport
b) protein synthesis
c) withstand turgor pressure
withstand turgor presure
What consists of a single interlinked molecule
a) cell membrane
b) nuclear envelope
c) bacterial endoplasmic reticulum
d) bacterial cell wall/sacculus
d) bacterial cell wall/sacculus
Most bacterial cell walls are made of
a) cellulose
b) cholesterol
c) peptidoglycan (murein)
d) ergosterol
c) peptidoglycan (murein)
Peptidoglycan consists of ___ and ___ bound to a peptide of 4-6 amino acids that can form cross-bridges to parallel glycan strands
NAG and NAM
A bacterial envelope is additional layers that provide ____ and _____
structural support and protection
____ have a thick cell wall and stain the primary stain color, purple
gram-positive bacteria
____ have thin cell walls that and stain the secondary stain color pink
gram-negative bacteria
the phylum firmicutes are examples of _____-______ bacteria and the phylum proteobacteria are examples of _____-______ bacteria
gram-positive: Firmicutes
gram negative: Proteobacteria
Which of the following is NOT a common trait of bacteria
a. RNA polymerase
b. Ribosomal RNAs
c. Translation factors
d. all of the above are common traits of bacteria
d. all of the above are common traits of bacteria
What makes most bacterial cell walls?
a. Chitin
b. Chloropyll
c. peptidoglycan
c. peptidoglycan
What are examples of Gram-Positive bactera
staphylococcus, streptococcus, listeria
Which bacteria are used in food production and preservation for things like yogurt and milk
Lactobacillus
What is another genera that is a fermentative bacteria
Streptococcus
What bacteria is used to make saurkraut
leuconostoc
What are the 2 key gram-positive bacteria that are nonsporulating bacillales and clostridiales
Listeria, staphylococcus
Staphylococcus is catalase-_______ and can tolerate ______
positive, drying
What are the key genera that are sporulating Bacillaes and Clostridiales (they make endospores)
Bacillus, Clostridium
Bacillus and Clostridium are gram-______ endospores
positive
What sporulating bacteria produces extracellular hydrolytic enzymes, antibiotics, and toxic insecticidal proteins
Bacillus
________ are pathogenic which causes botulism, tetanus, and gangrene
Clostridium
Tenericutes lack cells walls and include the genera _____
mycoplasma
Actinobacteria include _____ and _____ ______ _____
corneyform, propionic acid bacteria
A key genera of Actinobacteria is _______
corynebacterium
Actinobateria are mostly harmless commensals with the exception of _______
mycobacterium
Gram-______ Actinobacteria have acid-fast cell walls
positive
Aerial mycelia carry _____
arthrospores
Streptomyces is an example of gram-positive _____
actinobacteria
Gram-Negative Proteobacteria are the _____ group of cultured organisms
largest
Rhizobium, Agrobacterium, Rickettsias are examples of _____
alpha proteobacteria
Nitrosomonas and Neisseria gonorrhoeae are examples of _____
Beta Proteobacteria