Ex. 20 Gelatin Hydrolyis

Question 1

gelatin

Answer 1

protein produced by partial hydrolysis of collagen

Question 2

collagen

Answer 2

major component of connective tissues and tendons in humans and animals

Question 3

gelatin properties

Answer 3

solid at temp below 25C. above 25C is lquid

Question 4

gelatinase

Answer 4

proteolytic enzyme, acts to hydrolyze gelatin to aa, which are then small enough to cross membrane

Question 5

gelatin liquefication

Answer 5

degradation by gelatin. low temps will not be able to restore gel characteristics

Question 6

purpose of gelatin hydrolysis in labs

Answer 6

assess production of gelatin correlated w/ability of bacteria to break down collagen and spread throughout body of host

Question 7

how is gelatin diff from other solid media

Answer 7

the solidifying agent is also a substrate for enzymatic activity

Question 8

results of gelatinase +ive test

Answer 8

will liquify gelatin as they grow

Question 9

why imp to chill tubes before reporting gelatine liquefication

Answer 9

heat also liquifies gelatine, liquid state of warm Tryptic Soy Gelatin confused with results of bacterial liquefaction