Ex. 20 Enzymatic Attacks on Nitrogen containing substrates Flashcards
1
Q
urea hydrolysis
A
metabolic waste product toxic to living organism. urease catalyses hydrolyzation of urea into ammonia + C02
2
Q
urea NH2-CO-NH2 + H20 —>
A
C02 +2NH3
3
Q
why is urease helpful in i.d. of Proteus vulgaris
A
other bacteria produce urease but their rxn on substrate urea is slower than P. vulgarism. distinguish from other non lactose fermenting enteric organisms
4
Q
test medium for detecting urease
A
Urea broth. contains urea, yeast extract dye phenol red (pH indicator)
5
Q
results for urease
A
colour will change from red to yellow bc of production of basic ammonia
6
Q
why is potassium phosphate included in urea broth
A
resist alkalinization of medium due to peptone metabolism
