Evaluation of the Stud Dog Flashcards

1
Q

What is the first problem you see with 2 animals bred together that are too closely blood related (i.e. brother/sister, son/mom, etc.)?

A

Fertility - decreases the closer you get

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2
Q

What age is peak fertility in dogs?

A

2-4 years of age

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3
Q

What age is peak freezability of dogs?

A

2-5 years of age

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4
Q

T/F: Toy breed dogs may have peak fertility earlier than 24 months of age, whereas giant breed dogs may not have peak fertility until later.

A

T

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5
Q

What is the gold standard screening test for Brucella canis?

A

Cornell mutliplex test

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6
Q

Should you perform a physical exam before or after semen collection?

A

After

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7
Q

Why do overweight dogs have poor fertility?

A

Dogs put fat in their scrotum -> adds heat -> sperm don’t like to be hot -> poor fertility

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8
Q

Which of the following endocrine abnormalities affects fertility?

A. Hypothyroidism
B. Cushing’s
C. Addison’s
D. A & B only
E. B & C only
F. All of the above

A

E, Cushing’s & Addison’s

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9
Q

How long is the spermatogenic cycle?

A

60 days

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10
Q

T/F: If you give a stud dog antibiotics, it will only affect his fertility for the next 30 days.

A

F, it will affect his fertility for the next 60 days because spermatogenesis lasts 60 days.

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11
Q

T/F: It is safe to use Bravecto in breeding animals.

A

F!!!!! Decreases fertility & causes birth defects

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12
Q

What internal & external structures should be evaluated in all male dogs when present for a reproductive examination?

A
  • Penis & prepuce
  • Testicles, epididymes, spermatic cord, scrotum
  • Prostate gland
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13
Q

What is smegma & should you be concerned if a male dog presents with this?

A

Preputial discharge containing a mixture of WBCs & debris;

This occurs in intact & non-intact males & it is normal.

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14
Q

Why should you let a dog urinate & defecate outside prior to attempted collection?

A

Colon sits right on top - if they have not defecated yet, then it might be uncomfortable for them to give you a sample (& they may not even give you one because of it)

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15
Q

When collecting semen, why should you avoid lubrication containing chlorhexidine (i.e. KY lube)?

A

Because chlorhexidine is toxic to sperm cells

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16
Q

Describe how to perform a manual ejaculation for semen collection in a male dog.

A
  1. Grasp penile body behind the bulbus glandis
  2. Apply strong constant pressure (NOT friction) to create a tourniquette effect.
  3. Apply pulsatile pressure to bulbus glandis

Pulsations should begin with or without thrusting from him

17
Q

For penile blood flow, the blood will flow into the ________ ___________ and the ___________ from the female keeps that blood in during natural breedings.

A

Corpus spongiosum, occlusion

18
Q

What is in the first fraction of ejaculate?

A

Some prostatic fluid +/- urine (this is the presperm fraction)

19
Q

What are the 3 fractions of ejaculate?

A
  1. Presperm fraction
  2. Sperm-rich fraction
  3. Prostatic fraction
20
Q

What is in the second fraction of ejaculate?

A

Sperm-rich semen; comes from epididymes & testis

21
Q

What is in the third fraction of ejaculate?

A

Prostatic fluid only; this is the post-sperm fraction

22
Q

What is a non-invasive way of evaluating the prostatic fluid when you suspect disease?

A

Semen collection. Collect the first 2 fractions, remove the bag, and when the 3rd fraction begins, grab a red top tube.

23
Q

Describe the color of each of the ejaculate fractions.

A

Presperm = slightly cloudy
Sperm-rich = grayish white
Postsperm = crystal clear

24
Q

Do you need to keep the third fraction from the ejaculate?

A

No, unless you need to evaluate the prostate

25
You have just collected prostatic fluid that is orangish in color. What are you concerned for?
Prostatitis
26
When quickly evaluating motility, what type of movement from the sperm cells are you looking for?
Progressive linear movement; Done at 10x magnification for gross motility (40x for individual motility w/ coverslip) & look for a set of 10 sperm cells. Of those 10, how many are moving & how many are going in a linear progressive movement?
27
At what objective do you evaluate sperm morphology on a microscope?
100x under oil immersion
28
What is the minimum number of sperm cells you should evaluate morphology?
100 (classify as normal vs. abnormal (primary or secondary))
29
What 2 machines are both acceptable means for determining the concentration of ejaculate?
Nucleocounter (gold standard but expensive) & hemacytometer (best in private practice)
30
What is the minimum number of normal sperm per ejaculate in small breed dogs?
100-150 million
31
What is the minimum number of normal sperm per ejaculate in large breed dogs?
200 million
32
T/F: Cytology of ejaculate uses a modified Wright's stain. This stain is appropriate to use when looking at sperm morphology.
F
33
What is modified Wright's stain good for when evaluating cytology of ejaculate?
RBCs, WBCs, epithelial cells in ejaculate
34
Why should you compare cytology of prostatic fraction to a U/A?
Dogs are constantly retrograde ejaculating into their bladders; if something is going on with the bladder, then it is also going on with the prostate & vice versa
35
T/F: There is normal flora present on the penis, prepuce, and urethra in the male dogs.
T
36
What are the organisms that are considered normal flora on the penis, prepuce, & urethra in males?
Pasteurella, Staph, Strep, Mycoplasma. Sometimes will see E. coli too.
37
When is the only time that we perform cytology/culture on ejaculate?
When we suspect infection or something else going on. Hard to interpret results sometimes because of normal flora present.