Eukaryotic Transcription

Question 1

Basal transcription apparatus

Answer 1

Group of proteins that assemble near the start site and initiate minimal levels of transcription

Question 2

Transcriptional activator proteins

Answer 2

Bind to specific DNA sequences and increase transcription by stimulating the assembly of the basal transcription apparatus.

Question 3

Core promoter

Answer 3

Located upstream and is the site to which the basal transcription apparatus binds

Question 4

TATA box

Answer 4

TATAAA

Eukaryotic consensus sequence -25 to -30 bp upstream of the start site

Question 5

Regulatory promoter

Answer 5

Located upstream from the core promoter. Transcriptional activator proteins bind and directly or indirectly affect the rate at which transcription is initiated

Question 6

Enhancers

Answer 6

More distant sequences to which transcriptional activator proteins bind. The DNA loops out to interact with the machinery at the core promoter.

Question 7

TFIID

Answer 7

Transcription factor for RNA polymerase II - D. Binds to the TATA box which recruits RNA poly-II.

Question 8

Rat1 exonuclease

Answer 8

Degrades RNA after cleaveage of the mRNA, catching up to the RNA polymerase and halting transcription.