ETC Exercise 1 Flashcards
Acc to Barrington 2015, What is clearing?
Histological term
Process in which a specimen (or portion is rendered transparent)
According to Barrington 2015 What is Diaphonization?
Clearing an entire specimen or even an animal
Acc to Barrington 2015, How did previous methods used by vertucci of clearing work?
- Required an injection site to be made
- teeth cleaned in soon
- teeth decalcified in Nitri acid
- dehydrated in alcohol
- immersed in methyl salicylate
Acc to Barrington 2015, were the problems with prev methods of clearing?
- required an access to be gained and this could alter the internal structure
- the amount of internal structure revealed was dependant upon the amount of pressure used in the injection process
- Dye access depends on cleanliness of the canal content
- messy and time consuming
Barrington 2015 How must the teeth be stored prior to clearing with the new method?
- Must be extracted and dried within 24 hours of XLA
- no bleach advised
- any blood or PDL remnants left to dry on the outside
What acid and percentage was best used in the new method of clearing teeth Barrington 2015?
HCL 5-7%
Broadly speaking what are the stages of the new method by Barrington?
- Tooth collection
- Dried for 42 hours
- Decalcified in acid
- Dehydration: Immerse teeth in silica/cat litter
- immerse in oil for clearing
- Storage
What is the refractive index?
A measure of how fast light travels through the material
Why isn’t alcohol used in the new Barrington method?
- Alccohol can breakdown or destroy pulp remnants
- Not using alcohol allows air to replace any voids
- The drying method is a lot quicker without alcohol
What is the benefit to the level of detail seen in the new method in Barrington 2015?
- Microanatomy more easily seen from apical regions through to pulp space
- 3D visualisation better than that form CT scan
According to Robertson and Leeb 1982, What is the benefit of clearing teeth?
Allows you to see the microvasculature and canal morphology
What was the aim of Robertson and Leeb 1982 study?
to assess quality of obturation methods
According to Vertucci 2005, what are the diagnostic measures we have to identify number of canals present?
- pre op radiograph
- use of a sharp probe explore on pulp floor
- troughing of grooves
- staining of the pulp floor eg methylene blue
- Visualise canal bleeding points
- Microscope (can increase MB from 51% without scope to 82%)
According to Vertucci 2005 with no magnification use what percentage of MB2 are found?
18.2% Burley et al
According to Vertucci 2005 what is the pulp cavity comprised of?
Pulp chamber + root canal
Where is the pulp chamber located?
Within in the anatomic crown
According to Vertucci 2005 roughly what distance from root apex does apical foramen lie?
0-3mm
What direction do almost all root canals curve?
buccal lingual
According to Vertucci 2005, what is a lateral canal?
an accessory canal located in the coronal or middle third of root
According to Vertucci 2005, what part of the root canal do you see lateral canals?
Most commonly in apical third and lest cervically
According to Vertucci 2005, how are lateral canals formed?
By entrapment of periodontal vessels in HERS during calcification
What is the function of lateral canal?
To allow passage of irritants to the periodontal tissues
According to Vertucci 2005, how do furcal canals form?
Entrapment of periodontal vessels during fusion of diaphragm which forms floor of pulp chamber
According to Verticcu 2005 where are lateral canals most commonly located in mandib molars?
13% furcal
23% coronally
80% from distal root
Which teeth have a greater incidence of furcal canals: max or mandib molars?
Mandib