ETC and oxidative phosphorylation Flashcards
redox potential in ETC
o2 increases = steals e-
NADH = decrease redox potential as gives e- away easily
ETC complex I
NADH-CoQ reductase
largest
NADH transfers e- =NAD
passes e- along with 2H to coQ (reduced)
energy released used to pump 4H into intermembrane space
ETC complex II
oxidation of succinate to fumarate generate FADH2
remains coupled to succinate dehydrogenase and passed into FE-S clusters
oxidised and CoQ reduced
no H pumped
ETC complex III
reduced CoQ from I or II donate e- to clusters
2H released into inter membrane space
2 reduced cytochrome C formed
2H pumped in
ETC complex IV
cytochrome c oxidase
holds e- until 4
o2 accepts e- and h2o formed
oxidative phosphorylation
protons enter ATP synthase through Fo region
move c subunit of Fo = rotate
exit into matrix
in turn, y subbing rotates quick
ADP and PI bind to regions between subunits
rotation causes conformational change to F1 region catalysing ATP forming
number or H pumped by complex I
NADH = 4
FADH = n/a
number or H pumped by complex II
NADH = n/a
FADH = 0
number or H pumped by complex III
NADH = 4
FADH = 4
number or H pumped by complex IV
NADH = 2
FADH = 2
NADH proton pump total
10
FADH proton pump total
6
product yield by 1 glucose molecule in glycolysis
2 pyruvate
2 atp
2 nadh
product yield by 1 glucose molecule in link / PDH
2 nadh
2 acetyl co a
product yield by 1 glucose molecule in TCA cycle
3 nadh
1 fadh2
1 gtp
per cycle = x2
