Especially gluconeogenesis

Question 1

what does gluconeogenesis describe?

Answer 1

the process of making glucose out of pyruvate (from glycolysis or oxaloacetate (from Kreb’s cycle)

Question 2

why can’t glycolysis and gluconeogenesis occur simultaneously?

Answer 2

Simultaneous occurrence would result in a net negative consumption of ATP

Question 3

what 4 enzymes are unique to gluconeogenesis?

Answer 3

Fructose-1,6-bisphosphatase
Glucose-6-phosphatase
pyruvate carboxylase
phosphoenolpyruvate carboxykinase

Question 4

what does Fructose-1,6-bisphosphatase do?

Answer 4

converts Fructose-1,6-bisphosphate back to Fructose-6-phosphate by removing a Pi (via hydrolysis)

Question 5

what does Glucose-6-phosphatase do?

Answer 5

converts glucose-6-phosphate back to glucose via hydrolysis

Question 6

why does pyruvate have to go through the oxaloacetate intermediate rather than being directly converted back to phosphoenol pyruvate?

Answer 6

the conversion occurs in the mitochondrion and going through the intermediate is the only way to move the pyruvate into the mitochondria from the cytosol.

Question 7

which enzyme converts pyruvate to the oxaloacetate intermediate?

Answer 7

pyruvate carboxylase

Question 8

which enzyme converts oxaloacetate intermediate to phosphoenolpyruvate?

Answer 8

phosphoenolpyruvate carboxykinase