Epithelia

Question 1

Which type of epithelial tissue does NOT face the outside world?

Answer 1

Endocrine glands

Question 2

What are the functions of epithelial cells?

Answer 2

1. Protection
2. Selective Transport
3. Biochemical Modification and Processing
4. Sensory reception/transduction (e.g. taste buds)
5. Communication

Question 3

What is it called when something is transported through the apical and basal membranes of an epithelial cell?
What is it called if it goes between cells?

Answer 3

Transcellular transport

Paracellular Transport (between cells)

Question 4

During an experiment measuring the uptake of fMET to the connective tissue from the gut, when glucose was added, the transport max of fMET was exceeded. Whats going on?

Answer 4

Tight junctions had altered structure in presence of glucose that allowed for passive diffusion of fMET

Question 5

Which protein is important for forming tight junctions?

Answer 5

Claudins

Question 6

Which protein is important for forming adherens junctions?

Answer 6

Cadherins.

Cadherins connect to actin. They also recruit signaling complexes.

Question 7

What are the functions of adherens junctions?

Answer 7

Connect and Control cells

Question 8

Which protein is important for forming desmosome junctions?

Answer 8

Cadherins (different kinds) that connect to intermediate filaments

Question 9

What do focal adhesions attach to?

Answer 9

Basal Lamina and Actin

Question 10

What do Integrins do?

Answer 10

Connect cell to basal lamina

Question 11

A ______ gland secretes from the Apical surface. A _____ gland secretes from the basal lamina?

Answer 11

Exocrine

Endocrine

Question 12

What are the names of the secretory cells of the exocrine glands?

Answer 12

Acini Secretory units

Question 13

What are the characteristics of stem cells?

Answer 13

1. Divide
2. Renew
3. Differentiate

Question 14

What is the general wnt pathway in the colon?

Answer 14

Wnt–>beta catenin–> cell division

B-catenin–l differentiation

APC–l B-cat

Question 15

How does wnt signaling differ in the lung and colon?

Answer 15

Dec wnt in lung leads to cell division in the lung.

Question 16

Where do antibodies bind to specifically?

Answer 16

Epitopes

Question 17

Describe the purpose of having a primary and secondary antibody when preparing a slide?

Answer 17

Primary antibodies bind specifically to epitopes/proteins.

Secondary antibodies bind primaries and then fluoresce.

They are important for amplification and visualization as well as keeping cost down.