Enzymology Flashcards

1
Q

What are metabolic pathways

A

Sequence of chemical reactions where the product of one reaction serves as a substrate for the next

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2
Q

What two ways does the body store energy

A

Kinetic- energy at work

Potential- stored energy

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3
Q

What is a living system’s free energy

A

Energy that can do work under cellular conditions

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4
Q

What is anabolism

A

Involves the synthesis of larger molecules from smaller ones

Requires energy

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5
Q

What is catabolism

A

Involves the breakdown of larger molecules to smaller ones

releases energy

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6
Q

5 Important electron carriers

A
Flavoporteins
Cytochromes
Copper atoms in inner mitochondrial membrane
Ubiquinone 
Iron-sulphur proteins
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7
Q

how much energy is released in the hydrolysis of each bond in ATP

A

7.3Kcal

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8
Q

6 main classes of enzyme

A
Transferases
Ligases
Oxidoreductases
Isomerases
Hydrolases
Lyases
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9
Q

What do tranferases do

A

transfer functional groups between molecules

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10
Q

what do ligases do

A

join two molecules together

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11
Q

what do oxidoreductases do

A

move electrons between molecules

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12
Q

what do isomerases do

A

convert a molecules form one isomer to another

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13
Q

what do Hydrolases do

A

break bonds using water

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14
Q

what do lyases do

A

Break bonds without using water or oxidation

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15
Q

Describe enzymes and their function

A
Globular proteins with specific shapes
control rates of metabolic reactions
lower Ea needed to start reactions
Not consumed in chemical reactions
Substrate specific
Shape of active site determines
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16
Q

What influences reaction rates

A

Environmental conditions like temp., pH, substrate conc.
Co-factors and Co-enzymes
Inhibitors and activators

17
Q

What is an Apoenzyme

A

enzyme with co-factor removed, catalytically inactive

18
Q

What is a Holoenzyme

A

Enzyme with co-factor attached, catalytically active

19
Q

Non-reversible inhibitors of enzyme

A

Covalent attachment of the inhibitor to the enzymes