ENZYMOLOGY Flashcards

1
Q

How are enzymes measured?

A

based on their activity

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2
Q

factors affecting enzymatic reactions

A
  1. Coenzymes

2. Activators

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3
Q

General activator of enzymes

A

Ca, K

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4
Q

No. 1 enzyme activator

A

Ca

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5
Q

CK and ALP activator

A

Mg

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6
Q

LD activator

A

Zn

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7
Q

Amylase activator

A

Cl

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8
Q

measured in enzyme measurement

A

product + free enzyme

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9
Q

all that needs dehydrogenase

A

oxidoreductase

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10
Q

all kinases and transferase

A

Transferase

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11
Q

esterase and peptidase

A

Hydrolase

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12
Q

enzymes under esterase

A

ACP, ALP, Lipase, Cholinesterase

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13
Q

enzyme under esterase

A

LAP

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14
Q

Most important marker for obstructive jaundice

A

ALP

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15
Q

secondary pancreatic marker

A

Trypsin

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16
Q

most important Lyase

A

aldolase

17
Q

ALP Isoenzymes

A

Liver, bone, placental, intestinal

18
Q

marked increased in

A

Paget’s disease & Obstrctive jaundice

19
Q

what is ordered together with ALP to confirm an Obstructive jaundice

A

GGT

20
Q

MOST anodal ALP

A

Liver ALP

21
Q

least anodal ALP

A

intestinal ALP

22
Q

most heat stable ALP

A

Placental ALP

23
Q

most heat labile ALP

A

Bone ALP

24
Q

Heat fractionization test/ heat stability test

A

std. temp: 56’C
time: 10-15 mins
no reagent

25
Q

Chemical inhibition tests for ALP

A
  1. Phenylalanine
  2. 3M Urea
  3. Levamisole
26
Q

in Chemical inhibition tests for ALP, inhibits placental ALP

A

Phenylalanine

27
Q

inhibits bone ALP

A

3M urea

28
Q

Inhibits Major ALP

A

bone & liver

29
Q

Caranoplacental ALP

A
  1. Regan ALP

2. Nagao ALP

30
Q

Most heat stable Caranoplacental ALP

A

Regan ALP

31
Q

inhibited by Leucine and increased in Adenocarcinoma

A

Nagao ALP

32
Q

methods for ALP

A
  1. Bessy-Lowry Brock

2. Bower’s and comb (ALP & GGT)

33
Q

most specific method for ALP

A

Bessy-Lowry Brock

34
Q

significant marker for MI, lover dse, & skeletal muscle disease

A

Transferase

35
Q

Uses Karmen method (Aspartate)

A

Transferase

36
Q

major tissue source of ALT

A

Liver