Enzymes RP 5 Flashcards

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1
Q

Which enzyme digests starch in the human digestive system?

A

Amylase

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2
Q

Whats the first step of the practical?

A

To put a drop of iodine solution into each well of a spotting tile

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3
Q

How many test tubes should be used and what are the measurements for the solutions?

A

3 test tubes and 2cm³ for amylase and starch solution and 2cm³ pH 5 buffer solution.

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4
Q

What temperature should the test tubes be at in the water bath?

A

30°C for 10 minutes

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5
Q

After 30 seconds use the stir rod to transfer a drop of the solution, what shall we see?

A

It should to turn a blue-black color and we should repeat it till it stays orange. (Repeat several times using different pHs.)

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6
Q

Name one problem

A

Taking every 20 seconds as it only gives us an approximation

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