Enzymes RP 5 Flashcards
Which enzyme digests starch in the human digestive system?
Amylase
Whats the first step of the practical?
To put a drop of iodine solution into each well of a spotting tile
How many test tubes should be used and what are the measurements for the solutions?
3 test tubes and 2cm³ for amylase and starch solution and 2cm³ pH 5 buffer solution.
What temperature should the test tubes be at in the water bath?
30°C for 10 minutes
After 30 seconds use the stir rod to transfer a drop of the solution, what shall we see?
It should to turn a blue-black color and we should repeat it till it stays orange. (Repeat several times using different pHs.)
Name one problem
Taking every 20 seconds as it only gives us an approximation