Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Molecules to Cells > Enzymes (Ross) > Flashcards

Enzymes (Ross) Flashcards

You may prefer our related Brainscape-certified flashcards:
Organic Chemistry 101 flashcards
1
Q

At what values of ΔGo′ and Keq are the products favored?

A

ΔGo′ less than 0

Keq greater than 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

At what values of ΔGo′ and Keq are the reactants favored?

A

ΔGo′>0

Keq less than 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the equation for Gibb’s free energy?

A

ΔG=ΔGo′+RTlnKeq

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Name the four different catalytic strategies

A

Covalent catalysis
General acid-base catalysis
Catalysis by approximation
Metal ion catalysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the Michaelis-Menten equation?

A

Vo=Vm[S]/(Km+[S])

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What relation describes the fraction of active sites filled?

A

[ES]/[E]total=Vo/Vm=[S]/(Km+[S])

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the assumptions of the Michaelis-Menten equation?

A
  • Formation of ES complex
  • No back reaction from product buildup i.e. k4=0
  • Initial velocities used for analysis
  • Steady state assumption for [ES]
  • Negligible depletion of substrate i.e. [S]>[E]
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Define the Michaelis constant Km

A

Km=(k2+k3)/k1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Define the turnover number and what it measures

A

Turnover number=k3=kcat

Measure of the catalytic ability of the enzyme when the active sites are saturated

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Define catalytic efficiency

A

Kcat/Km

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is a perfect enzyme?

A

One whose catalytic efficiency is limited only by the rate of diffusion of the substrate to the enzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What the characteristics of the Lineweaver-Burk plot?

A

x-int=-1/Km
y-int=1/Vm
slope=Km/Vm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

For two-substrate kinetics, name the three types of mechanisms

A

Ordered sequential
Random sequential
Ping-pong (double replacement)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Describe the changes in Vm, Km, and Kcat/Km for competitive inhibitors

A

Vm stays the same
Km increases
kcat/Vm decreases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Describe the changes in Vm, Km, and Kcat/Km for noncompetitive inhibitors

A

Vm decreases
Km stays the same
kcat/Vm decreases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Describe the changes in Vm, Km, and Kcat/Km for uncompetitive inhibitors

A

Vm decreases
Km decreases
kcat/Vm stays the same

17
Q

Describe the changes in the Lineweaver-Burk plot for competitive inhibitors

A

Y-intercept stays the same, slope increases

18
Q

Describe the changes in the Lineweaver-Burk plot for noncompetitive inhibitors

A

X intercept stays the same, slope increases

19
Q

Describe the changes in the Lineweaver-Burk plot for uncompetitive inhibitors

A

Slope stays the same, line moves higher

20
Q

Describe the effect of irreversible inhibitors on Michaelis-Menten parameters

A

Vm decreases

Km stays the same

21
Q

Name the three types of irreversible inhibitors

A

Group-specific reagents
Affinity labels
Suicide inhibitors

22
Q

Why don’t allosteric enzymes fit into the Michaelis-Menten model?

A

Show sigmoidal relationship between velocity and substrate

Molecules to Cells (34 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions