Enzymes Revision

Question 1

importance of KM

Answer 1

index for affinity
isoenzymes have different KM
If there is an enzyme that can bind to 1+ substrate, diff KM for each substrate
if there is inhibitor, it will higher KM and lower affinity

Question 2

KM

Answer 2

Substrate concentration that leads to 1/2 VMAX

Question 3

sulfonamide/sulfanilamide

• used in
• competes with
• to inhibit

Answer 3

used in treatment of bacterial infection

competes with PABA to inhibit folic acid

Question 4

allopurinol

• used in
• competes with
• to inhibit

Answer 4

used in treatment for gout

competes with hypoxanthine to inhibit uric acid formation

Question 5

dicoumarol & warfarin

• used in
• competes with
• to inhibit

Answer 5

anticoagulant
lower activation of blood clotting
competes with vit k to inhibit active form of vit k

Question 6

statins

• used in
• competes with
• to inhibit

Answer 6

used for cholesterol

competes with HMG COA to lower plasma cholesterol

Question 7

chemical group inhibitors - SH - sulfhydrl

Answer 7

Include: oxidizing agents - oxidizes SH into 2SS

salts of heavy metals like HgCl2

Question 8

chemical group inhibitors -OH (hydroxyl) ex

Answer 8

aspirin for anti flammatory