Enzymes, metabolism, cell evolution (Q2) Flashcards
Life in built on _________________that transform energy from one for to another
Chemical reactions
The chemical reactions of life are called _______________
Metabolism
Types of metabolism
Forming and breaking bonds between molecules
Dehydration synthesis and synthesis are ____________
Anabolic reactions (form bonds)
Hydrolysis and digestion are ______________
Catabolic reactions (break bonds)
Exothermic reactions ____________ energy
Release
Endothermic reactions ____________ energy
Require (input of)
Catabolism (hydrolysis like digesting polymers) is an _____________ reaction
Exothermic
Anabolism (dehydration synthesis like building polymers) is an _____________ reaction
endothermic
The amount of energy needed to destabilize bonds between molecules
Activation energy
The molecular currency of intracellular energy transfer; stores and transports chemical energy within cells
ATP
In order to break bonds in large biomolecules, which are stable, there needs to be an ___________ _____________ __ ___________ (activation energy) which is gained by absorbing it; combustion
initial input of energy
Substances that accelerate a chemical reaction; reduces the amount of energy to start a reaction
Catalysts
Catalysts in cells are __________
Enzyme
Most enzymes are __________
Proteins
Enzymes function is to _____________ chemical reactions
facilitate
Enzymes are required for most ______________ reactions; they are in control of reactions in life
biological
Enzymes are highly specific, there are ____________ of different ones
thousands
Reactant which binds to the enzyme
Substrate
End result of reaction; anything made as a result of an enzymatic reaction
Product
Enzyme’s catalytic site; specific region of an enzyme where a substrate fits into ______________
Active site
Each enzyme works with a specific substrate (chemical fit between active site and substrate)
Reaction specific
Single enzyme molecule can catalyze thousands or more reactions per second (unaffected by reaction)
Not consumed in reaction
Any condition that affects protein structure like temperature, pH and salinity
Affected by cellular conditions
Enzymes are named for the__________ they catalyze
reaction
Breaks down sucrose
Sucrase
Breaks down proteins
Proteases
Breaks down lipids
Lipases
Builds DNA by adding nucleotides to DNA strand
DNA polymerase
Breaks down proteins (polypeptides)
Pepsin
In the lock and key model, the “key” is the __________ while the lock is the __________ (3D structure of the active site)
Substrate
Enzyme
1st four factors that affect enzymes
Enzyme concentration
Substrate concentration
Temperature
pH
2nd three factors that affect enzymes
Salinity
Activators
Inhibators
The higher the concentration of enzymes, the ________ the reaction rate; collides qith substrate more frequently
higher
____________ can be a limiting factors, since not all enzymes can find one, causing the reaction rate to level off
Substrate
Temperature for human enzymes
35 to 40 degrees; average temperature is 37
Temperature that produces the greatest number of molecular collisions
Optimum T
When heat increases beyond optimum T, enzymes undergo __________, or lose their 3D shape
Denaturation