Enzymes+ Colorimetry Flashcards
For molecules to react they need
Collision with enough energy and appropriate orientation
Activation energy is
Energy needed for two molecules to surpass the reaction energy barrier
Enzyme substrate reaction forms an..
Intermediate, with lower activation energy
Catalyst vs enzyme
Catalyst for wide processes
Enzymes very selective, specific reactions by acting on specific or interrelated substrates
Enzymes features
Unchanged at the end of the reaction
Enzymes structure affects rate
Many has cofactors
Factors affecting activity
Temp> high energy, more frequent collisions, successful collisions increase, optimum
Ph> optimum, other will cause intramolecular and intermolecular alterations in structure
Enzyme conc>high =high till reaction ends
Substrate conc>till enzyme is limiting factor
Spectrophotometer is
An instrument to measure absorbance of light at various wavelengths
UV, visible, IR
Componenets
Light
Monochromator
Slit (1cm)
Cuvette with sample
Detector
Amplifier
Output
Beer lambert law
Explain + law
Linear relation between concentration and absorption
A=€lc
Absorbance
Absorption coef
Path length 1cm
Conc
Standard curve is
A type of graph used as a quantitative research technique
Standard curve equation
Y=mx+b
Y> absorbance
X> conc
Kit calculation
Sample absorbance/ standard absorbance
* Standard conc
What is a blank
Zero reference for spectrophotometer calibration/ solution with no sample
Standard solution
Solution with known conc to determine the unknown
Colorimeter vs spectrophotometer
Low cost,high
Low sensitivity,high
Fixed wavelength,wide
Visible light,UV/IR
Not complex,complex
Color intensity, wavelength
