Enzymes and Biological Molecules Flashcards

1
Q

What are organic molecules, and why are they important in living organisms?

A

Organic molecules contain carbon and hydrogen atoms and are essential for providing energy and raw materials for growth and tissue repair.

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2
Q

What are the four main groups of organic molecules found in living organisms?

A

Carbohydrates, lipids, proteins, and nucleic acids.

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3
Q

What property allows carbon atoms to form large and diverse structures in organic molecules?

A

Carbon atoms bond strongly with other carbon atoms, forming chains and ring structures.

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4
Q

What is the study of organic and inorganic molecules in living organisms called?

A

Biochemistry

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5
Q

Define hydrolysis and condensation reactions in living organisms.

A

Hydrolysis: Large molecules are broken down into smaller ones by the addition of water.

Condensation: Large molecules are built from smaller ones by the removal of water.

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6
Q

Why is the solubility of molecules like glucose and amino acids important in living organisms?

A

Soluble molecules can be easily transported and absorbed, while insoluble molecules like starch and fats are better suited for storage.

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7
Q

Give one important function of each group of organic molecules:

A

Carbohydrates: Provide energy for life processes.
Lipids: Store energy and form cell membranes.
Proteins: Support growth, repair, and enzymatic activities.
Nucleic acids: Store and transmit genetic information.

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8
Q

Why might nucleic acids, proteins, or carbohydrates be considered the most important molecules in living cells?

A

Nucleic acids: Essential for genetic information storage and transmission.
Proteins: Crucial for enzymes, structure, and cellular functions.
Carbohydrates: Provide energy to sustain life processes.

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9
Q

What are biological molecules often called, and why?

A

They are called organic molecules because they were discovered in living organisms.

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10
Q

Which elements do organic molecules always contain?

A

Carbon and hydrogen.

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11
Q

Why can organic molecules form a wide variety of structures?

A

Carbon atoms bond strongly to each other, forming chains and rings.

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12
Q

What are the two main needs for organic molecules in living organisms?

A

Providing energy for life processes and raw materials for growth and tissue repair.

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13
Q

How do living organisms obtain the organic molecules they need?

A

Through nutrition.

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14
Q

What are the four main groups of organic molecules in living organisms?

A

Carbohydrates, lipids, proteins, and nucleic acids.

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15
Q

Name one inorganic molecule essential for living organisms.

A

Water

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16
Q

What is metabolism?

A

The sum of all chemical reactions in living organisms.

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17
Q

What are large organic molecules typically made up of?

A

Smaller, similar molecules called subunits.

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18
Q

What is hydrolysis?

A

A reaction where large molecules are broken down into smaller ones by the addition of water.

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19
Q

What is condensation in biochemistry?

A

A reaction where large molecules are built from smaller ones by the removal of water.

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20
Q

How can living organisms rearrange molecules for their needs?

A

By using hydrolysis to break down molecules and condensation to build them up in new combinations.

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21
Q

Why is solubility important for molecules like glucose and amino acids?

A

Soluble molecules can be transported and absorbed efficiently in the body.

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22
Q

Why are insoluble molecules like starch and fats important?

A

They are better suited for storage in living organisms.

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23
Q

What function does each group of organic molecules serve?

A

Carbohydrates: Provide energy.
Lipids: Store energy and form membranes.
Proteins: Support growth and enzymatic activities.
Nucleic acids: Store and transmit genetic information.

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24
Q

Why are nucleic acids considered vital?

A

They store and transmit genetic information essential for life.

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25
Q

Why are proteins often called the most important molecules?

A

They function as enzymes, structural components, and facilitate cellular processes.

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26
Q

Why might carbohydrates be essential for life?

A

They are the primary energy source for life processes.

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27
Q

What happens to starch in a rat’s cells during digestion?

A

It undergoes hydrolysis to break down into glucose subunits, which are then used to build glycogen through condensation reactions.

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28
Q

What are monosaccharides, and give an example?

A

Monosaccharides are the simplest carbohydrates, e.g., glucose, which is soluble and easily transported in blood and cells.

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29
Q

What is the main function of monosaccharides in cells?

A

They are the primary source of energy for many cells.

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30
Q

How are polysaccharides formed, and give two examples?

A

Polysaccharides are formed by combining many monosaccharides. Examples include starch and glycogen.

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31
Q

Why are polysaccharides good for energy storage?

A

They are insoluble, making them ideal for energy storage and forming structures like cellulose in cell walls.

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32
Q

What elements do carbohydrates contain?

A

Carbon (C), hydrogen (H), and oxygen (O).

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33
Q

What is the difference between fats and oils?

A

Fats are solid at room temperature, while oils are liquid.

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34
Q

Why are fats and oils good for energy storage?

A

They are insoluble in water and form barriers between watery environments.

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35
Q

How are lipids formed?

A

By the condensation of three fatty acid molecules with one glycerol molecule.

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36
Q

What is the biological significance of lipids being insoluble?

A

They act as excellent energy stores and form barriers, like cell membranes.

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37
Q

What are proteins made of, and how are they formed?

A

Proteins are made of long chains of amino acids joined by condensation reactions.

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38
Q

How many different amino acids exist, and what determines a protein’s function?

A

There are 20 different amino acids. The sequence and shape of the amino acids determine a protein’s function.

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39
Q

Give an example of two protein structures and their functions.

A

Long and thin: Keratin in hair and nails.
Spherical: Enzymes or antibodies with active or binding sites.

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40
Q

Why are amino acids soluble, and what advantage does this provide?

A

Amino acids are soluble, enabling them to be transported in living organisms and react in the cytoplasm.

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41
Q

What elements do proteins sometimes contain besides carbon, hydrogen, and oxygen?

A

Nitrogen (N) and sometimes sulfur (S).

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42
Q

What is the simplest organic molecule, and what is its formula?

A

Methane, CH₄.

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43
Q

What determines the biological function of a molecule?

A

Its shape and structure.

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44
Q

What are nucleic acids, and what elements do they contain?

A

Nucleic acids, such as DNA, contain carbon, hydrogen, oxygen, nitrogen (N), and phosphorus (P).

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45
Q

What is the structure of DNA, and what is its function?

A

DNA is a double helix. It carries genetic information in the sequence of its bases, passed from generation to generation.

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46
Q

How does the sequence of DNA bases affect an organism?

A

It forms a code that instructs cells or organisms to perform specific tasks.

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47
Q

How do hydrolysis and condensation relate to carbohydrates, lipids, and proteins?

A

Hydrolysis: Breaks down large molecules into subunits.
Condensation: Joins subunits to form larger molecules.

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48
Q

What is the objective of biochemical tests?

A

To describe simple chemical tests for molecules in living organisms.

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49
Q

Why might scientists test for glucose in a urine sample?

A

The presence of glucose in urine may indicate diabetes.

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50
Q

Why would an environmental scientist test for starch in factory outflows?

A

To check for contamination or residues from food production.

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51
Q

What is a key characteristic of fats and oils that affects biochemical testing?

A

They are insoluble in water, so aqueous solutions cannot be made for testing.

52
Q

What is the biochemical test for lipids called?

A

The emulsion test.

53
Q

What are the steps of the emulsion test?

A

Add 2 cm³ of ethanol to the unknown solution and shake gently.

Pour the mixture into a test tube containing an equal volume of distilled water.

54
Q

What indicates the presence of a lipid in the emulsion test?

A

The formation of a milky-white emulsion.

55
Q

Why is the emulsion test a physical rather than a chemical test?

A

It relies on the insolubility of lipids in water, forming a visible emulsion.

56
Q

What is the test for Vitamin C, and what reagent is used?

A

The test for Vitamin C uses DCPIP, a blue dye that loses its color in the presence of Vitamin C.

57
Q

How does the number of drops of Vitamin C solution relate to its concentration?

A

Few drops: Strong Vitamin C solution.
Many drops: Weak Vitamin C solution.

58
Q

Why is a control important in biochemical tests?

A

A control ensures that results are valid by confirming the test is working and that solutions are not contaminated.

59
Q

How is a positive control conducted?

A

A solution known to contain the substance is tested to confirm the test gives a positive result.

60
Q

How is a negative control conducted?

A

A sample of water is tested to confirm it gives a negative result, ensuring no contamination.

61
Q

What reagent is used to test for protein, and what is a positive result?

A

Biuret reagent; a mauve/purple color indicates the presence of protein.

62
Q

How do you test for starch, and what is a positive result?

A

Add iodine solution; a deep blue-black color indicates the presence of starch.

63
Q

How do you test for glucose (a reducing sugar), and what is a positive result?

A

Add Benedict’s reagent and heat in a boiling water bath for 2–3 minutes; an orange/brick-red color indicates glucose is present.

64
Q

Why must biochemical tests be carried out under the same conditions for comparisons?

A

To ensure results are consistent and comparable.

65
Q

What conditions must be kept consistent when comparing Benedict’s tests?

A

Equal volumes of unknown solutions.
Equal volumes of Benedict’s reagent.
Same temperature and time of heating.

66
Q

Why is the DCPIP test useful for Vitamin C testing?

A

It provides a visual indication of Vitamin C concentration based on the number of drops required to decolorize the dye.

67
Q

What does a negative result in a biochemical test suggest?

A

The tested substance is not present, or the solution was not contaminated.

68
Q

What does a positive result for the Biuret test indicate?

A

The presence of proteins in the sample.

69
Q

Why is iodine used in the starch test?

A

It reacts with starch to produce a characteristic blue-black color.

70
Q

Why is heating necessary in the Benedict’s test?

A

Heat enables the chemical reaction that causes the color change in the presence of glucose.

71
Q

What is the role of distilled water in biochemical testing?

A

It acts as a negative control to verify no contamination.

72
Q

What does an orange/brick-red color indicate in the Benedict’s test?

A

The presence of glucose (reducing sugar).

73
Q

How can glucose levels be compared in different solutions?

A

By performing Benedict’s tests under identical conditions for all samples.

74
Q

What are anabolic reactions?

A

Anabolic reactions build up large molecules from smaller ones and usually require an input of energy.

75
Q

What is an example of an anabolic reaction?

A

The condensation of glucose molecules into the polysaccharide glycogen in liver and skeletal muscle cells.

76
Q

What are catabolic reactions?

A

Catabolic reactions break down large molecules into smaller ones and often release energy.

77
Q

What is an example of a catabolic reaction?

A

The breakdown of glucose into carbon dioxide and water by respiration in liver and skeletal muscle cells.

78
Q

What are enzymes?

A

Enzymes are proteins that function as biological catalysts, speeding up reactions without being changed by them.

79
Q

What are substrates and products in enzyme-catalyzed reactions?

A

Substrates are the molecules that react, and products are the molecules produced in the reaction.

80
Q

What determines whether glucose is built into glycogen or broken down?

A

The presence or absence of specific enzymes determines this.

81
Q

What are intracellular enzymes?

A

Enzymes that work inside cells, such as catalase (breaks down hydrogen peroxide) and phosphorylase (builds glucose into starch).

82
Q

What are extracellular enzymes?

A

Enzymes made inside cells but released to work outside, such as lipase (breaks down fats) and amylase (converts starch to maltose).

83
Q

What does it mean that enzymes are specific?

A

Most enzymes work on only one kind of substrate. For example, proteases act on proteins but not on carbohydrates or lipids.

84
Q

What is the lock-and-key hypothesis?

A

The active site of an enzyme fits specific substrate molecules exactly, forming an enzyme-substrate complex.

85
Q

How does the lock-and-key mechanism work?

A

Substrate molecules bind to the enzyme’s active site, react to form products, and the products leave the active site, freeing the enzyme for reuse.

86
Q

What is the role of the active site in an enzyme?

A

The active site allows the enzyme to act as a catalyst by bringing substrate molecules closer together and lowering the energy needed for the reaction.

87
Q

How do enzymes lower activation energy?

A

Enzymes reduce the energy needed for a reaction, making it more likely to occur.

88
Q

What is an example of a digestive enzyme?

A

Lipase, which breaks down fats into fatty acids and glycerol.

89
Q

Why can enzymes be reused?

A

Enzymes are not consumed during the reaction; they can catalyze many reactions repeatedly.

90
Q

What happens if a substrate doesn’t match the active site of an enzyme?

A

The enzyme cannot catalyze the reaction, as it is specific to its substrate.

91
Q

What is an example of enzyme specificity?

A

Proteases only break down proteins and do not act on lipids or carbohydrates.

92
Q

What is the importance of enzyme specificity in metabolism?

A

It ensures that specific reactions occur, controlling the metabolic pathways in cells.

93
Q

How does temperature affect enzyme activity?

A

Enzyme activity increases with rising temperature due to faster substrate and enzyme movement, but high temperatures can denature enzymes.

94
Q

What happens to enzymes when they are denatured?

A

Denatured enzymes lose their three-dimensional shape and can no longer bind to their substrate.

95
Q

What is the optimum temperature for most human enzymes?

A

Around 37°C.

96
Q

What is the optimum temperature for most plant enzymes?

A

Around 25°C.

97
Q

Why does enzyme activity decrease at high temperatures?

A

High temperatures cause excessive vibration of enzyme molecules, leading to denaturation.

98
Q

Can denaturation due to high temperature be reversed?

A

Denaturation is usually irreversible.

99
Q

How does pH affect enzyme activity?

A

Changes in pH can alter an enzyme’s three-dimensional shape and denature it, affecting its ability to bind to substrates.

100
Q

What is the optimum pH for pepsin, and where does it work?

A

Pepsin has an optimum pH of around 2.0 and works in the stomach.

101
Q

What is the optimum pH for amylase, and where does it work?

A

Amylase has an optimum pH of around 7.5 and works in the mouth and small intestine.

102
Q

What are enzyme activators?

A

Molecules that make it more likely for an enzyme to bind to its substrate, e.g., chloride ions for salivary amylase.

103
Q

What are enzyme inhibitors?

A

Molecules that make it harder for an enzyme to bind to its substrate, e.g., cyanide ions block active sites in enzymes involved in respiration.

104
Q

Why are enzymes useful in industry, food preparation, and medicine?

A

Enzymes are specific, reusable, and effective under mild conditions, making them valuable for various applications.

105
Q

Give an example of an enzyme used in medicine.

A

An enzyme from snake venom can break down blood clots.

106
Q

What is the relationship between enzyme activity and temperature as shown in the graph?

A

Enzyme activity increases with temperature, peaks at the optimum temperature, and then decreases due to denaturation.

107
Q

How do pH extremes affect enzyme activity?

A

Extreme pH values can denature enzymes, preventing them from functioning.

108
Q

Why is it important for living cells to maintain suitable conditions for enzymes?

A

To prevent denaturation and ensure efficient biochemical reactions.

109
Q

What is the role of chloride ions in enzyme activity?

A

Chloride ions act as activators for salivary amylase, enhancing its function.

110
Q

How does cyanide act as an enzyme inhibitor?

A

Cyanide blocks the active sites of enzymes involved in respiration, preventing their function.

111
Q

What are the key steps of the scientific method?

A

Observation, hypothesis formation, prediction, experimentation, data analysis, and drawing conclusions.

112
Q

What is a hypothesis?

A

A possible explanation for an observation, used as a basis for making predictions and designing experiments.

113
Q

What is the purpose of a control experiment?

A

To ensure that changes in the dependent variable are caused by the independent variable and not by other factors.

114
Q

What reaction does the enzyme catalase catalyze?

A

The breakdown of hydrogen peroxide into oxygen and water.

115
Q

How is the activity of catalase measured in the described experiment?

A

By the amount of oxygen produced, measured using a manometer.

116
Q

What is the independent variable in the catalase experiment?

A

Temperature

117
Q

What is the dependent variable in the catalase experiment?

A

The volume of oxygen released in a fixed length of time.

118
Q

Why are variables such as the variety of potato and the number of potato discs controlled?

A

To ensure a fair test and valid results by eliminating the influence of these factors on enzyme activity.

119
Q

What type of variable is temperature in the catalase experiment?

A

A continuous variable.

120
Q

What type of variable is the variety of potato?

A

A categoric variable, as it can be one of several different sorts.

121
Q

What type of variable is the number of potato discs?

A

A discrete variable, as it can only be whole numbers.

122
Q

Why is taking a series of results and calculating the mean important?

A

It improves accuracy by reducing the impact of any single inaccurate result.

123
Q

How is the temperature varied in the catalase experiment?

A

It is controlled by the experimenter as the independent variable.

123
Q

Why is hydrogen peroxide concentration controlled in the experiment?

A

To ensure that only the independent variable (temperature) affects the dependent variable (oxygen production).

124
Q

What does the manometer measure in the catalase experiment?

A

The change in fluid level, indicating the amount of oxygen produced.

125
Q

What ensures that an experiment is valid and fair?

A

Controlling variables, using a control experiment, and repeating measurements to calculate a mean.