enzymes Flashcards

1
Q

What are enzymes?

A

Enzymes are biological catalysts.

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2
Q

What do enzymes do?

A

They speed up the rate of chemical reactions without themselves being chemically changed at the end of the chemical reactions.

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3
Q

Can enzymes be reused?

A

Yes

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4
Q

Are enzymes effective in large or small concentrations?

A

Enzymes are effective in small concentrations.

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5
Q

What is a substrate?

A

Substrate is the molecule which an enzyme acts on.

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6
Q

What is the product?

A

Product is the result of a chemical reaction catalysed by an enzyme.

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7
Q

What is the active site?

A

It is the part of an enzyme where substrate(s) bind(s).

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8
Q

What is the enzyme-substrate complex?

A

It is formed when substrate molecule(s) bind to the active site of the enzyme.

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9
Q

Define catabolic reactions.

A

Catabolic reactions consist of chemical reactions which break down complex molecules into simpler molecules.

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10
Q

Define anabolic reactions.

A

Anabolic reactions consist of chemical reactions which build up simple molecules into complex molecules.

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11
Q

What are most enzymes made of?

A

Most enzymes are proteins with a unique 3-dimensional structure.

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12
Q

Where are proteins synthesized?

A

In ribosomes. (Refer to chapter on Cells.)

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13
Q

What part of the enzyme binds to the substrate?

A

The active site.

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14
Q

Why is each enzyme specific to a substrate?

A

Each active site can only allow specific substrate(s) to fit in.

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15
Q

What causes the specificity of an enzyme?

A

The complementary shape between the substrate and the active site.

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16
Q

What is activation energy?

A

The energy that must be supplied to the reactant molecules for them to react.

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17
Q

In what form is activation energy often supplied?

A

In the form of heat.

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18
Q

What happens when reactant molecules absorb thermal energy?

A

They move faster and collide more frequently and forcefully in the correct orientation.

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19
Q

How does thermal energy affect bonds within molecules?

A

It increases the chance that bonds will break.

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20
Q

Why is heating inappropriate in biological systems?

A

High temperatures denature proteins and kill cells.

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21
Q

Why can’t organisms use heating to speed up chemical reactions?

A

Heating speeds up all reactions

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22
Q

How do enzymes speed up chemical reactions?

A

They lower the activation energy required to start a chemical reaction.

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23
Q

What is the first step in an enzymatic reaction?

A

Effective collision between specific substrate(s) and enzyme at the correct orientation.

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24
Q

What forms when a substrate enters the active site? How does that help?

A

An enzyme-substrate complex that lowers activation energy.

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25
What happens after the chemical reaction occurs?
Products are formed and dissociate from the enzyme.
26
What happens to the enzyme after the reaction?
It is chemically unchanged and ready for another cycle.
27
What are the two models that explain enzyme action?
Lock and key model and Induced fit model.
28
In the lock and key hypothesis, what does the enzyme represent?
Lock
29
In the lock and key hypothesis, what does the substrate represent?
Key
30
Why is the substrate compared to a key?
It has a shape complementary to the active site of the enzyme.
31
What forms when the substrate fits into the enzyme’s active site?
An enzyme-substrate complex.
32
What happens to products after the reaction in the lock and key model?
They no longer fit into the active site and are released.
33
How is the active site described in the induced fit model?
Complementary in shape but not a perfect fit to the substrate.
34
What happens to the active site when the substrate binds in the induced fit model?
It changes shape to fit the substrate more tightly.
35
What is formed after the substrate binds in the induced fit model?
An enzyme-substrate complex.
36
What happens after the products are formed in the induced fit model?
They are released as they no longer fit into the active site.
37
How do enzymes speed up chemical reactions?
By lowering the activation energy.
38
How much faster can enzyme-catalysed reactions be compared to uncatalysed ones?
10³ to 10⁸ times faster.
39
What happens to enzymes after a reaction?
They remain chemically unchanged.
40
Why are only small amounts of enzymes needed?
Because they can be reused and are effective in small concentrations.
41
Why are enzymes specific in their action?
Because of their 3D shape and an active site that is complementary to a specific substrate.
42
What determines enzyme specificity?
The complementary shape between the substrate and the active site.
43
What is the optimum temperature for an enzyme?
The temperature at which the rate of enzyme activity is at its maximum.
44
What happens to enzyme activity at low temperatures?
Enzymes are less active.
45
Why does enzyme activity increase as temperature rises?
Because kinetic energy increases
46
What happens to the rate of reaction every 10°C rise in temperature (until optimum)?
It doubles.
47
What happens to enzymes beyond their optimum temperature?
They denature and lose their 3D shape.
48
What is denaturation?
When the enzyme loses its 3D shape and active site
49
Why is denaturation irreversible?
Because the enzyme cannot regain its function even if temperature is lowered.
50
What is the optimum pH of an enzyme?
The pH at which enzyme activity is at its maximum.
51
What happens to enzyme activity if pH deviates from the optimum?
The rate of reaction decreases.
52
What happens to enzymes at extreme pH values?
They denature.
53
What is the effect of denaturation due to pH?
The active site is no longer able to bind to the substrate.
54
What is a limiting factor?
A factor that directly affects the rate of a chemical reaction if its quantity is changed.
55
What happens when enzyme concentration increases at low levels?
Rate of reaction increases due to more effective collisions.
56
Why does rate of reaction plateau at high enzyme concentration?
Because there are not enough substrates to occupy all the enzyme active sites.
57
When is enzyme concentration no longer a limiting factor?
When adding more enzyme does not increase the reaction rate.
58
What happens when substrate concentration increases at low levels?
Rate of reaction increases due to more effective collisions.
59
Why does the reaction rate plateau at high substrate concentration?
Because all active sites of enzymes are saturated.
60
When is substrate concentration no longer a limiting factor?
When enzyme active sites are fully occupied.