enzymes

Question 1

enzymes

Answer 1

• Enzymes are catalytically active biological macromolecules
  enhancing reaction rate, globular proteins,

Question 2

enzyme advantages

Answer 2

• Greater reaction specificity: selectivity of substrates and products (not
  side products)
• Milder reaction conditions in cells: mostly 37C and pH  7
• Higher reaction rates: in a biologically useful time frame
• Capacity for regulation: control of biological pathways

Question 3

kinectic mechanism

Answer 3

the order of binding of substrates and release of products

Question 4

inhibitors

Answer 4

decrease enzyme activity

Question 5

inactivators

Answer 5

irreversible inhibitors

Question 6

reversible inhibitors

Answer 6

• to the free enzyme and prevent the binding of the substrate
• to the enzyme-substrate complex and prevent the reaction

Question 7

competitive inhbition

Answer 7

bind only to free enzyme, active site, does not affect catalysis or vmax, increase in Km similar to substrate, can be reversed by adding more substrate.

Question 8

uncompetitive inhibition

Answer 8

bid only to enzyme substrate complex, only binds to ES complex, does not affect substrate binding, inhibits catalysitc function (vmax)

Question 9

noncompetitive inhibtion

Answer 9

bind equally well to free enzyme and enzyme substrate, binds to regulatory site, inhibits both substrate binding and catalysis. decrease in vmax, change in Km, mixed inhibitors that bind equally well to E
and to ES with no change in Km