Enzymes Flashcards
The appearance of LD-1/LD-2 flipped pattern and the presence of CK-MB isoenzyme on electrophoresis is highly suggestive of?
Myocardial Infarction
classification/sub-classification of Alanine amino transferase
Aminotransferase
classification/sub-classification of creatine phosphokinase
Phosphotransferase
classification/sub-classification alkaline phosphate
Esterase
classification/sub-classification of amylase
Glycosidase
classification/sub-classification of lactate dehydrogenase
Oxidoreductase
Disease causing increase in ACP
Hairy cell leukemia
Disease causing increased in CK-MB
Myocardial infarction
Disease causing increased in amylase
Pancreatitis
Disease causing increased in AST
Muscle trauma
Disease causing increased in lipase
Pancreatitis
In enzyme kinetics, what do we call the speed of enzyme reaction that initially increases as the substance increases?
First order kinetics
Which of the following methods for alkaline phosphatase uses buffered phenolphthalein phosphate as substrate?
Klein-Babson-Reed
One of the following is a coenzyme in the LDH catalyzed reaction
NAD/NADH
The De Ritis ratio is a very useful indicator of the etiology of liver disease. What is the indication if the De Ritis ratio is very high?
Alcoholic liver disease
The following laboratory results is suggestive of what condition?
I. Alkaline phosphatase = extremely high II. Direct bilirubin = normal
III. Gamma glutamyl transferase=normal IV. Alanine transferase= normal
Paget’s disease
One of the following is not a reason for increased enzyme activity.
Reaction lacks co-factors
Tissue source of ACP
Rbc
Tissue source of lipase
Pancreas
Tissue source of AST
Skeletal muscle
Tissue source of amylase
Pancreas
Tissue source of CK-MB
Myocardium
Which of the following functions as a coenzyme in transaminase reaction?
Pyridoxal phosphate
The term to describe loss of activity of the enzyme because of physical processes such as heat, mechanical agitation, irradiation is called?
Denaturation
In amylase determination, the method that measures the disappearance of starch substrate is:
Amyloclastic
Which of the following enzymes exhibit the least tissue specificity?
LDH
What ALP isoenzyme has the same properties as the Regan isoenzyme?
Placental
LDH levels are increased in the following disease except:
Pancreatitis
catalyzes the removal of a group of atoms from the substrate
Lyases
Catalyzes group transfer reaction
Transferase
Catalyzes hydrolytic reaction
Hydrolase
Catalyzes the reduction reactions
Oxido-reductase
Catalyzes reactions involving isomeration
Isomerase
This theory allows some degree of conformational change after binding of substrate and active site so as to form a stronger association.
Induce fit
From the items listed below, identify the reason for the increase in urinary amylase activity in acute pancreatitis.
There is an increased renal clearance of amylase
One of the following is the term used when an enzyme activity being measured is coupled with a colorimetric reaction.
Coupled enzyme reaction
The enzyme that acts on several phosphate ester substrates and exhibit low tissue specificity is:
ALP
One of the following is the term used when an enzyme activity being measured is coupled with a colorimetric reaction.
Coupled enzyme reaction
The enzyme that acts on several phosphate ester substrates and exhibit low tissue specificity is:
ALP
Which of the following is the substrate of choice for measuring acid phosphatase activity?
Thymolphthalein monophosphate
Which amylase of the following methods for amylase determination measures the amount of starch hydrolyzed within a specified time?
Amylometric
The end color of starch using iodine after its completely hydrolyzed.
Brown
