Enzymes Flashcards
What are enzymes?
biological catalyst, usually proteins that speeds up a chemical reaction
Substrate
recognized by & binds to an enzyme
reacts with active site
active site
pocket that binds enzyme to substrate
Induced for hypothesis
enzymes changes its shaped to better accommodate a substrate
Enzyme cycle
binds to substrate
converts substrate to one or more products
unchanged enzyme repeats process with another substrate molecule
Cofactor
a non protein group that binds to an enzyme & is essential for catalytic activity
coenzyme
organic molecules that acts as a cofactor of an enzyme
Enzyme Activity Factors: Enzyme & substrate concentration
excess substrate: rate of reaction is proportional
enzyme at constant intermediate concentration: increasing substrate increases till the saturation point
Enzyme Activity Factors: Inhibitors
lower the rate of reactions
competitive inhibitors: bind to active site & block substrates
noncompetitive inhibitors: bind somewhere other than active site & block enzyme activity (change shape)
Enzyme Activity Factors: Allosteric control
allosteric site is a binding site and changes shape of enzymes
activators: stabilize enzyme causing active site to have a high affinity
inhibitors: stabilizes inactive form of the enzyme & changes shape
Enzyme Activity Factors: Feedback Inhibition
regulation of pathway
prevents cellular resources from being wasted
Enzyme Activity Factors: pH
optimal ph where enzymes work best
increase or decrease in pH decreases the rate
Enzyme Activity Factors: Temperature
rising temperatures means rate increase
between 0 to 40 reaction rate doubles
above 40 denaturing occurs