Enzymes Flashcards
enzymes are made of
proteins, amino acids
enzymes act as
biological catalysts that increase the rates of reactions and is not changed by the reaction
how do enzymes get their shape
- the sequence and types of animo acids in its structure
- the shape is determined in different ways as it helps them to do different functions
enzymes are
specific as each substrate fits with a different substrate in its active site, which can be reused
any reaction needs
energy so it can occur, called activation energy. when the substrate fits with the enzyme, creating the E-S complex they lower the activation needed for it to occur, making it easier to occur and so speeds up the reaction
how does enzymes help with a reaction
first a disaccharide (sucrose) is formed by fructose and glucose chemically bonded, the substrate fits into the enzyme (E-S complex), where the disaccharide is broken using water molecules to become 2 monosaccharides by hydrolysis. finally the sucrose that was broken down into fructose and glucose (the products) are the result of a chemical reaction, the reactants (sucrose) products (fructose and glucose)
enzymes are important because
they are needed in digestion, respiration, photosynthesis and much more chemical reactions needed for survival
lactose breaks down
to sugars in milk (disaccharides)
lactase is an enzyme that
breaks down lactose in to galactose and glucose
why does temp affect the reate of enzyme activity
temp is a variable that reflects how much kinetic energy particles have (celsius and fahrenheit) so in a graph, it has a form or a J
how a graph represents temp affecting rate of enzyme activity
- first there will be an exponential increase as particles gain energy and collide more
- then reaches optimum temp, where the bonds break and enzyme changes shape
- enzyme activity decreases drastically as it denatures
explain temp graph
as temp increases, enzyme activity increases as they gain more kinetic energy and collide more. the enzyme works best at optimum temp but temp continues increasing and after that, the enzymes denatures and the activity decreases. high temp lead to loss of enzyme activity
why does pH affect the reate of enzyme activity
pH is a variable that reflects acidity in a solution, therefore in a graph, it is represented as a bell shape
how a graph represents pH affecting rate of enzyme activity
- first there drastic increase when pH levels increase,
- it reaches optimum pH,
- the active site changes shape and denatures, so there is a gradual decrease as pH levels continue to increase
explain pH graph
the effect of pH on enzyme activity is that the optimum pH is where the enzymes works bests, but as it continues to rise after the optimum, it causes hydrogen bonds to weaken and the enzyme changes shape, it denatures and there is a gradual decrease. when the pH becomes to acidic or to alkaline, the activity decreases