Enzymes Flashcards
Catalyst
Chemical substance that increases the rate of reaction without being changed or used up.
Enzymes
Proteins that function as biological catalysts, used in digestion or building up larger molecules. They are produced intercellularly in ribosomes. They work intercellularly in respiration or extracellularly in digestion.
Lock and key model
Enzymes work on reactions by fitting onto the active site of the substrate. Enzymes and substrates are complementary to each other, and fit to form enzyme-substrate complexes. Enzymes then leave forming products.
Properties of enzymes
They are all proteins. They can be reused. They are specific to one substrate: they can only catalyse one chemical. They denature at high temperatures and unsuitable PHs, due to hydrogen bonds breaking down - this is irreversible. They work best at a particular PH and temp.
Effect of temperature
Rate of reaction increases with temperature as enzymes gain more kinetic energy and collide more often. The peak on a graph is the optimum temperature: temp. that rate of reaction is highest at. After optimum temperature is reached, enzymes become denatured. Less ES complexes are formed, rate of reaction gradually decreases to a stop. Human enzymes usually have an optimum temp. of 37.
Effect of PH
There is a small range of of PH that an enzymes can work at. Rate of reaction increases as you get closer to optimum PH - the peak on a graph is the optimum PH -rate of reaction is highest - beyond optimum PH - denatured. less ES complexes - rate of reaction decreases.